Self-assembly of artificial architectures in living cells — design and applications

Wound healing strategies based on nanoparticles incorporated in hydrogel wound patches

“…185 Self-assembled NPs represent the most potential cost-efficient and high-throughput practical technique to nano-production. 175 Self-assembled NPs especially used for wound healing strategies can be classied according to its material types, including metal, peptide amphiphiles, peptide-based selfassembled NPs, and elastin-like polypeptides. The fabrication methods can be divided into three major mechanisms: spontaneous (non-covalent) self-assembly, enzyme-catalysed selfassembly and chemical/physical cross-linked self-assembly.…”

“…174 The self-assembly processes can also be stimulated artificially to control biological mechanisms in living cells. 175 Consequently, nanotechnological advances around self-assembled systems present features of structural adjustability, biocompatibility and functionality that are of great significance. 176,177 They focus on improving treatment strategies, 178 vaccination, 179,180 wound healing, 177,181 DNA origami-nanorobot for drug nanodelivery system, 182,183 disease detection applications, 172 neural tissue repair, 184 and other nanoscale therapeutic delivery systems.…”

Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system

“…In recent decades, DNA nanomaterials have been developed as promising carriers for drug delivery (23)(24)(25)(26). The unique properties of DNA nanomaterials are conducive to increasing blood circulation (27,28), promoting tissue penetration (29,30), and accelerating cellular uptake (31,32).…”

Engineering living cells with cucurbit[7]uril-based supramolecular polymer chemistry: from cell surface engineering to manipulation of subcellular organelles

“…Strategies for organelle-targeted intracellular synthesis and in situ selfassembly have been established to controllably interfere with cellular behaviors, including cell migration, proliferation, and apoptosis. [31][32][33][34][35] Synthetic polymers are one of the most popular articial materials used in living cell engineering, mainly because of their accessibility and structural diversity. 36,37 Nevertheless, the current demand for precision, modularity, and complexity creates further challenges in developing advanced polymeric systems.…”

“…Strategies for organelle-targeted intracellular synthesis and in situ self-assembly have been established to controllably interfere with cellular behaviors, including cell migration, proliferation, and apoptosis. 31–35…”