Self-association of caseinomacropeptide in presence of CaCl2 at neutral pH: Calcium binding determination

Reply to the ‘Comment on “Deciphering calcium-binding behaviors of casein phosphopeptides by experimental approaches and molecular simulation”’ by D. Horne, Food Funct., 2022, 13, https://doi.org/D2FO00808D

“…3,4 Phosphate solutions have been com-monly adopted to obtain the binding capacities of peptides with calcium by many researchers. [5][6][7][8] Moreover, Dr. Horne suggested that omitting phosphate salts in this method could solve the problem. However, phosphate is an irreplaceable ingredient to suspend the binding complexes of peptides and calcium in the water and to precipitate the "unbound" calcium in the bottom, enabling the calculation of the binding capacities of peptides with calcium.…”