Self-Bonded Hydrogel Inverse Opal Particles as Sprayed Flexible Patch for Wound Healing

Abstract

Wound healing is an important issue for regenerative medicine. Attempts in this area tend to develop functional wound patches to promote the healing. Here, we present self-bonded hydrogel inverse opal particles as sprayed flexible patch for wound healing. Such particles were fabricated by infusing drugs-loaded gelatin (GT) and carrageenan (CG) pregel into inverse opal scaffolds, which were composed of biocompatible hyaluronic acid methacryloyl (HAMA) and gelatin methacryloyl (GelMA) with graphene oxide quantum dots (GO QDs) doping. Due to the photothermal conversion capability of GO QDs and temperature reversible phase-changing performance of GT/CG, the hybrid particles could undergo GT/CG liquid transformation under the near-infrared (NIR) irradiation, which made them adhere to each other and finally form a flexible patch. Following by the phase-change of GT/CG hydrogel, the encapsulated drugs were also controllably released from the inverse opal scaffold. As the inverse opal scaffolds of the hybrid particles were maintained, their drug release induced refractive index changes could be detected as visual structural color shifting, which could be utilized to monitor their delivery processes. Based on these features, we have demonstrated that the self-bonded particles, administered in the form of spray, could be applied for wound tissue healing and drug delivery monitoring. These results indicate that the self-bonded hydrogel particles have potential value as a multifunctional patch for clinical applications.

Inhibition of Bacteria In Vitro and In Vivo by Self-Assembled DNA-Silver Nanocluster Structures

“…Y-DNA showed a positive effect on wound healing, which may be attributed to the good biocompatibility and antioxidant capacity of DNA. , Masson staining was used to observe the collagen formation (blue) during wound healing (Figure e). Collagen plays a significant role in the proliferation and differentiation of tissue cells, regarded as an important indicator for skin tissue reconstruction and wound repair . The images showed that the collagen fiber deposition in three groups on day 5 was lower than that on day 15.…”

Bioactive hydrogels based on polysaccharides and peptides for soft tissue wound management

“…[53][54][55][56] Due to the lack of adhesive action, excessive swelling, and premature degradation upon contact with tissue, the mechanical properties of HA-based hydrogels are generally poor as wound repairing materials. These issues can be addressed by chemical modification of HA to improve the cross-linking degree of networks, and the interpenetration and entanglement of the polymer chains, including catechol [57][58][59][60][61][62][63] and pyrogallol 57,64 for musselinspired catechol chemistry through hydrogen-bond interaction and metal ions coordination, aldehydes from periodate oxidation of HA 60,[65][66][67][68][69] and photoactive o-nitrobenzyl [70][71][72][73] for Schiff base reaction, methacrylate 61,62,71,[74][75][76] for photoinitiated free radical polymerization, phenylboronic acid 77,78 for glucose-sensitive dynamic borate bond with polyphenol groups, etc.…”

“…84,86,[128][129][130] In addition, catechol [130][131][132][133][134] /pyrogallol [135][136][137] are introduced into gelatinbased hydrogels for further adhesion enhancement, and methacrylate gelatin (GelMA) hydrogels are preferred for convenience in some cases. 76,126,134,[138][139][140][141] Building on the above methods, researchers have recently developed a series of excellent wound management strategies. For example, Huang et al 139 designed an octopus suction cup-mimicking wound patch with selective adhesive property and individualized design possibility (Fig.…”

“…However, frequent and long-term hospitalization for monitoring can be impractical due to the high financial burden and poor patient adherence. Some efforts on wound monitoring [155][156][157][158] and responsive release of bioactive agents to wound sites 23,66,76,159 have been made, but hydrogels with the integrity of sensing and ondemand delivery in response to a dynamic wound environment remain a challenge. In addition, the development of customized bioactive hydrogels in the treatment of individual wounds can be a breakthrough in the future.…”

Aggressive strategies for regenerating intervertebral discs: stimulus-responsive composite hydrogels from single to multiscale delivery systems

“…For drug delivery, HAMA–GelMA composite hydrogels doped with graphene oxide quantum dots (GO-QDs) were constructed as a drug delivery system, and the photothermal conversion capability of GO-QDs gave the scaffold the ability to guide wound tissue healing and drug delivery monitoring. 144 Whereas Pluronic F127 diacrylate (F127DA) nano micelles enhance the compressive properties of HAMA hydrogels and exhibit low swelling ratios, effectively promoting cartilage regeneration in vivo , 66 this multiscale delivery system based on HAMA with ready-to-use particles is expected to be an excellent strategy for regenerative IVD.…”