Self-Diffusion in Chitosan Networks: From a Gel−Gel Method to Fluorescence Recovery after Photobleaching by Fringe Pattern

Payet, Linda; Ponton, Alain; Léger, Liliane; Hervet, H.; Grossiord, J. L.; Agnely, Florence

doi:10.1021/ma801192q

Cited by 24 publications

(19 citation statements)

References 51 publications

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“…Figure summarizes the different permeability of the FITC‐labeled dextran with increasing molecular weight through the capsule walls with varied porosities. Figure a indicates that the capsules made of biggest microspheres (M1) showed an almost impermeable even for the FITC‐labeled dextran with the lowest molecular weight (FD20) tested, which means that the biggest microspheres integrated in the capsule are tightly patterned with pores presumably <3 nm . However, the capsules made of microspheres around 500 nm (M3) under the same conditions ( C PCL = 50 g L −1 ) are observed to be permeable to dextrans with molecular weights of 20 and 70 kDa and non‐permeable for dextrans with molecular weight of 150 kDa and above (Figure b).…”

Section: Resultsmentioning

confidence: 94%

Stimuli‐Responsive Capsules Prepared from Regenerated Silk Fibroin Microspheres

Cheng

Teasdale

Brüggemann

2014

Macromolecular Bioscience

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Microcapsules are synthesized via the self-assembly of silk-fibroin microspheres with polycaprolactone in a colliodosome preparation procedure. The microsphere building blocks with controlled diameter are prepared from the naturally occurring biopolymer, silk fibroin, and then act as stabilizers for oil-in-water (O/W) emulsion and organized themselves on the surface of chloroform droplets to form the capsules. The concentration of the protein-based microspheres and the binding polycacrolactone is used to tailor the size, as well as the permeability of the resultant capsules. Furthermore, microencapsulation of fluorescently labeled macromolecules (20-2000 KDa) is demonstrated. The permeability of the capsules is observed to be stimuli responsive, making it possible to incorporate a pH-triggered payload release from the capsules. The relatively simple preparation of capsules with controlled dimensions and tunable permeability, alongside the biocompatibility and biodegradability of both polymer components makes these promising materials for potential use in smart drug-delivery systems.

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Section: Resultsmentioning

confidence: 94%

Stimuli‐Responsive Capsules Prepared from Regenerated Silk Fibroin Microspheres

Cheng

Teasdale

Brüggemann

2014

Macromolecular Bioscience

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“…The diffusion coefficients for several MWs of FITC-dextran dissolved in PBS at 25°C have been measured[17] and give similar results to one study of FITC-dextran in water. [18] Averaging interpolated results from four studies[17-20] that measured the diffusion coefficient D of 10kDa FITC-dextran in water and PBS at 25°C yields D = 1.3×10 −6 cm 2 s −1 . The particular values interpolated from each study ranged from 0.9 to 2.0×10 −6 cm 2 s −1 due to differences in the degree of branching and polydispersity of the dextrans used in the studies.…”

Section: Methodsmentioning

confidence: 96%

Convection-driven generation of long-range material gradients

Hancock

et al. 2010

Biomaterials

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Natural materials exhibit anisotropy with variations in soluble factors, cell distribution, and matrix properties. The ability to recreate the heterogeneity of the natural materials is a major challenge for investigating cell-material interactions and for developing biomimetic materials. Here we present a generic fluidic approach using convection and alternating flow to rapidly generate multi-centimeter gradients of biomolecules, polymers, beads and cells and cross-gradients of two species in a microchannel. Accompanying theoretical estimates and simulations of gradient growth provide design criteria over a range of material properties. A poly(ethyleneglycol) hydrogel gradient, a porous collagen gradient and a composite material with a hyaluronic acid/gelatin cross-gradient were generated with continuous variations in material properties and in their ability to regulate cellular response. This simple yet generic fluidic platform should prove useful for creating anisotropic biomimetic materials and high-throughput platforms for investigating cell-microenvironment interaction.

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“…21 FEM computation used an initial reagent concentration in the source chamber and microchannel of 40 μ g/mL in order to match the experimental conditions, and an initial concentration in the sink chamber of 0 μ g/mL. Reported diffusivity values of Dextran 10,23,30,38 were substituted into the model in order to generate spatial concentration profiles within the microchannel that most accurately modeled those measured experimentally. Computational predictions illustrated in a linear, steady-state gradient along the 7-mm-long microchannel after t = 70 h (Fig.…”

Section: Methodsmentioning

confidence: 99%

Role of Epidermal Growth Factor-Triggered PI3K/Akt Signaling in the Migration of Medulloblastoma-Derived Cells

et al. 2012

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Medulloblastoma (MB) is the most common brain cancer diagnosed among children. The cellular pathways that regulate MB invasion in response to environmental cues remain incompletely understood. Herein, we examine the migratory response of human MB-derived Daoy cells to different concentration profiles of Epidermal Growth Factor (EGF) using a microfluidic system. Our findings provide the first quantitative evidence that EGF concentration gradients modulate the chemotaxis of MB-derived cells in a dose-dependent manner via the EGF receptor (EGF-R). Data illustrates that higher concentration gradients caused increased number of cells to migrate. In addition, our results show that EGF-induced receptor phosphorylation triggered the downstream activation of phosphoinositide-3 kinase (PI3K)/Akt pathway, while its downstream activation was inhibited by Tarceva (an EGF-R inhibitor), and Wortmannin (a PI3K inhibitor). The treatment with inhibitors also severely reduced the number of MB-derived cells that migrated towards increasing EGF concentration gradients. Our results provide evidence to bolster the development of anti-migratory therapies as viable strategies to impede EGF-stimulated MB dispersal.

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Self-Diffusion in Chitosan Networks: From a Gel−Gel Method to Fluorescence Recovery after Photobleaching by Fringe Pattern

Cited by 24 publications

References 51 publications

Stimuli‐Responsive Capsules Prepared from Regenerated Silk Fibroin Microspheres

Stimuli‐Responsive Capsules Prepared from Regenerated Silk Fibroin Microspheres

Convection-driven generation of long-range material gradients

Role of Epidermal Growth Factor-Triggered PI3K/Akt Signaling in the Migration of Medulloblastoma-Derived Cells

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