Self-enhanced electrochemiluminescence of luminol induced by palladium–graphene oxide for ultrasensitive detection of aflatoxin B1 in food samples

Xia, Mengke; Yang, Xue; Jiao, Tianhui; Oyama, Munetaka; Chen, Quansheng; Chen, Xiaomei

doi:10.1016/j.foodchem.2022.132276

Cited by 35 publications

(17 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, the commonly used labeling enzymes are alkaline phosphatase (ALP) and horseradish peroxidase (HRP) (Li et al, 2017). Unlike the above two types of analysis, ECLIA is an analytical technique in which an antigen or antibody is labeled with an ECL agent and undergoes specific CL due to electron transfer in an electric field (Xia et al, 2022).…”

Section: Chemiluminescent Immunosensorsmentioning

confidence: 99%

“…At present, the commonly used labeling enzymes are alkaline phosphatase (ALP) and horseradish peroxidase (HRP) (Li et al., 2017). Unlike the above two types of analysis, ECLIA is an analytical technique in which an antigen or antibody is labeled with an ECL agent and undergoes specific CL due to electron transfer in an electric field (Xia et al., 2022). Since the CL‐type immunosensor is a combination of ultrasensitive CL analysis and highly selective immunoassay, it not only overcomes the disadvantages of complex instruments and background interference, but also avoids the radioisotopes contamination of radioimmunoassay.…”

Section: Principles On Single‐mode Immunoassay Of Mycotoxinsmentioning

confidence: 99%

See 1 more Smart Citation

Advances in immunoassay‐based strategies for mycotoxin detection in food: From single‐mode immunosensors to dual‐mode immunosensors

Chen

Qileng

Liang

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Mycotoxin contamination in foods and other goods has become a broad issue owing to serious toxicity, tremendous threat to public safety, and terrible loss of resources. Herein, it is necessary to develop simple, sensitive, inexpensive, and rapid platforms for the detection of mycotoxins. Currently, the limitation of instrumental and chemical methods cannot be massively applied in practice. Immunoassays are considered one of the best candidates for toxin detection due to their simplicity, rapidness, and cost‐effectiveness. Especially, the field of dual‐mode immunosensors and corresponding assays is rapidly developing as an advanced and intersected technology. So, this review summarized the types and detection principles of single‐mode immunosensors including optical and electrical immunosensors in recent years, then focused on developing dual‐mode immunosensors including integrated immunosensors and combined immunosensors to detect mycotoxins, as well as the combination of dual‐mode immunosensors with a portable device for point‐of‐care test. The remaining challenges were discussed with the aim of stimulating future development of dual‐mode immunosensors to accelerate the transformation of scientific laboratory technologies into easy‐to‐operate and rapid detection platforms.

show abstract

Section: Chemiluminescent Immunosensorsmentioning

confidence: 99%

Section: Principles On Single‐mode Immunoassay Of Mycotoxinsmentioning

confidence: 99%

Advances in immunoassay‐based strategies for mycotoxin detection in food: From single‐mode immunosensors to dual‐mode immunosensors

Chen

Qileng

Liang

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…Electrochemiluminescence (ECL) is chemiluminescence that originates from the electron transfer between species generated on the surface of electrodes [ 97 , 98 ], which simultaneously has the dual advantages of electrochemical analysis and chemiluminescence such as high sensitivity, good reliability, simple operation, and fast analysis process [ 99 , 100 ], so it has been widely used in the biomedical field [ 101 , 102 ], food safety assessment [ 103 , 104 ], environment monitoring [ 105 , 106 ], and other fields of biomolecule detection [ 107 , 108 ]. Based on the merits of ECL, Liu et al combined the advantages of spherical nucleic acids with CRISPR technology, in which a Y-shaped DNA structure constructed from helper DNA (A1), prostate cancer biomarker α-methylacyl coenzyme A racemase (AMACR) adaptors, and DNA activators are loaded onto gold nanoparticle-modified Fe 3 O 4 magnetic beads (Au@Fe 3 O 4 MBs) [ 36 ].…”

Section: Aptamer-assisted Crispr/cas-based Protein Detectionmentioning

confidence: 99%

Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection

et al. 2022

View full text Add to dashboard Cite

CRISPR is an acquired immune system found in prokaryotes that can accurately recognize and cleave foreign nucleic acids, and has been widely explored for gene editing and biosensing. In the past, CRISPR/Cas-based biosensors were mainly applied to detect nucleic acids in the field of biosensing, and their applications for the detection of other types of analytes were usually overlooked such as small molecules and disease-related proteins. The recent work shows that CRISPR/Cas biosensors not only provide a new tool for protein analysis, but also improve the sensitivity and specificity of protein detections. However, it lacks the latest review to summarize CRISPR/Cas-based biosensors for protein detection and elucidate their mechanisms of action, hindering the development of superior biosensors for proteins. In this review, we summarized CRISPR/Cas-based biosensors for protein detection based on their mechanism of action in three aspects: antibody-assisted CRISPR/Cas-based protein detection, aptamer-assisted CRISPR/Cas-based protein detection, and miscellaneous CRISPR/Cas-based methods for protein detection, respectively. Moreover, the prospects and challenges for CRISPR/Cas-based biosensors for protein detection are also discussed.

show abstract

“…Due to its dramatic poisoning effects on the human body, the rapid and accurate detection of AFB 1 is in high demand in food processing [ 7 , 8 ]. So far, various analytical methods such as high-performance liquid chromatography, thin-layer chromatography and enzyme-linked immunosorbent assays have been developed to detect AFB 1 [ 9 , 10 , 11 , 12 , 13 ]. However, most of these methods involve costly equipment and complicated sample processing and suffer from several drawbacks such as a low efficiency and/or false-positive results, which greatly restrict their widespread use [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1

et al. 2022

View full text Add to dashboard Cite

High-performance electrochemical biosensors for the rapid detection of aflatoxin B1 (AFB1) are urgently required in the food industry. Herein, a multi-scaled electrochemical biosensor was fabricated by assembling carboxylated polystyrene nanospheres, an aptamer and horseradish peroxidase into a free-standing carbon nanofiber/carbon felt support. The resulting electrochemical biosensor possessed an exceptional performance, owing to the unique structures as well as the synergistic effects of the components. The 3D porous carbon nanofiber/carbon felt support served as an ideal substrate, owing to the excellent conductivity and facile diffusion of the reactants. The integration of carboxylated polystyrene nanospheres with horseradish peroxidase was employed as a signal amplification probe to enhance the electrochemical responses via catalyzing the decomposition of hydrogen peroxide. With the aid of the aptamer, the prepared sensors could quantitatively detect AFB1 in wine and soy sauce samples via differential pulse voltammetry. The recovery rates of AFB1 in the samples were between 87.53% and 106.71%. The limit of detection of the biosensors was 0.016 pg mL−1. The electrochemical biosensors also had excellent sensitivity, reproducibility, specificity and stability. The synthetic strategy reported in this work could pave a new route to fabricate high-performance electrochemical biosensors for the detection of mycotoxins.

show abstract

Self-enhanced electrochemiluminescence of luminol induced by palladium–graphene oxide for ultrasensitive detection of aflatoxin B1 in food samples

Cited by 35 publications

References 35 publications

Advances in immunoassay‐based strategies for mycotoxin detection in food: From single‐mode immunosensors to dual‐mode immunosensors

Advances in immunoassay‐based strategies for mycotoxin detection in food: From single‐mode immunosensors to dual‐mode immunosensors

Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection

A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1

Contact Info

Product

Resources

About