Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S-allele-associated proteins

Ai, Yunjun; Singh, Anuradha; Coleman, Craig E.; Ioerger, Thomas R.; Kheyr-Pour, A.; Kao, Teh Hui

doi:10.1007/bf00198857

Cited by 116 publications

(101 citation statements)

References 16 publications

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“…Nineteen different S haplotypes were identified based on the results of genetic crosses described in the "Materials and Methods." One of these 19 S haplotypes was designated S 1 because its SI behavior was identical to that of one of the three previously identified S haplotypes of P. inflata, S 1 , S 2 , and S 3 (Ai et al, 1990). All the other 18 S haplotypes were novel and thus designated S 4 through S 21 .…”

Section: Identification Of S Haplotypes Andmentioning

confidence: 99%

“…This size range was what would be expected of cDNAs containing the coding sequence for S-RNases (except for the region upstream of C2) plus the 3Ј-non-coding sequence and the poly(A) tail. This major DNA band for each S haplotype was cloned and individual colonies from each transformation were analyzed by DNA gel blotting using a radiolabeled probe containing cDNAs for S 1 -, S 2 -, and S 3 -RNases of P. inflata (Ai et al, 1990). Under low stringency membrane washing conditions, cDNA clones for eight of the 17 S haplotypes (S 1 , S 5 , S 6 , S 8 , S 12 , S 13 , S 15 , and S 19 ) hybridized to the probe, whereas for the remaining nine S haplotypes (S 4 , S 7 , S 9 , S 10 , S 11 , S 16 , S 17 , S 20 , and S 21 ), none of the cDNA clones hybridized to the probe.…”

Section: Identification Of S Haplotypes Andmentioning

confidence: 99%

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Evidence That Intragenic Recombination Contributes to Allelic Diversity of the S-RNase Gene at the Self-Incompatibility (S) Locus in Petunia inflata

Wang¹,

Hughes

Tsukamoto

et al. 2001

Plant Physiology

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(T.T., T.A.)For Solanaceae type self-incompatibility, discrimination between self and nonself pollen by the pistil is controlled by the highly polymorphic S-RNase gene. To date, the mechanism generating the allelic diversity of this gene is largely unknown. Natural populations offer a good opportunity to address this question because they likely contain different alleles that share recent common progenitors. We identified 19 S haplotypes from a natural population of Petunia inflata in Argentina, used reverse transcriptase-polymerase chain reaction to obtain cDNAs for 15 alleles of the S-RNase gene, and sequenced all the cDNAs. Phylogenetic studies revealed that five of these alleles and two previously identified alleles form a major clade, and that the 5Ј region of S 19 allele was derived from an ancestor allele closely related to S 2 , whereas its 3Ј region was derived from an ancestor allele closely related to S 8 . A similar evolutionary relationship was found among S 3 , S 12 , and S 15 alleles. These findings suggest that intragenic recombination contributed to the generation of the allelic diversity of the S-RNase gene. Two additional findings emerged from the sequence comparisons. First, the nucleotide sequence of the S 1 allele identified in this work is completely identical to that of the previously identified S 1 allele of a different origin. Second, in the two hypervariable regions HVa and HVb, thought to be involved in determining S allele specificity, S 6 and S 9 alleles differ only by four nucleotides, all in HVb, resulting in two amino acid differences. The implications of these findings are discussed.

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Section: Identification Of S Haplotypes Andmentioning

confidence: 99%

Section: Identification Of S Haplotypes Andmentioning

confidence: 99%

Evidence That Intragenic Recombination Contributes to Allelic Diversity of the S-RNase Gene at the Self-Incompatibility (S) Locus in Petunia inflata

Wang¹,

Hughes

Tsukamoto

et al. 2001

Plant Physiology

Self Cite

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“…(continued) reported in the solanaceous S-RNases are shown over the alignment of NA-S2 [S2-RNase of Nicotiana alata (DDBJ/EMBL/GenBank Accession No. U08860)] and PI-S1 [S1-RNase of Petunia inflata (Ai et al, 1990)] and boxed. Conserved residues in rosaceous and all S-RNases are indicated by bold face and asterisks, respectively.…”

Section: Protein Assaymentioning

confidence: 99%

Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum).

Yamane

Tao

Sugiura

1999

Plant Biotechnology

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This report demonstrates the presence of S-RNases in style of self-incompatible Japanese plum (Prunus salicina Lindl.) and presents information about cDNA sequencese ncoding the S-RNases. Two stylar glycoprotein spots that were found in highly basic zone of 2D-PAGE gel with Mr of about 30 kDa shared similarity in N-terminal amino acid sequence and immunological characteristics with other rosaceous S-RNases and appeared to be S-RNases of Japanese plum. Deduced amino acid sequences from the cDNA clones encoding these glycoproteins contained two active sites of T2/S type RNases and five regions conserved among other rosaceous S-RNases. Genomic DNA blot and RNA blot analyses using the cDNA clones as probes and phylogenetic analysis based on the deduced amino acid sequences from the cDNAs all indicated that the cDNA clones encoded S-RNase corresponding to the stylar glycoproteins detected on 2D-PAGE gel. Phylogenetic analysis based on the deduced amino acid sequences from the cDNAs supported the hypothesis that the S-RNases of Rosaceae have diverged after the divergence of subfamilies but before the divergence of species.

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“…of the mature protein as well as several other conserved sequences, as depicted in Figure 4. This information allowed the cloning of cDNAs corresponding to further S alleles of N. alata and S alleles from Petunia inflata (Ai et al, 1990), Solatium chacoense (Xu et al, 1990), P hybrida (petunia; Clark et al, 1990), Solanum tubemsum (potato; Kaufmann et al, 1991), and Lycopersiconperuvianum (Tsai et al, 1992). Alignment of all these sequences (Tsai et al, 1992) shows that, overall, ~16°/o of the amino acids are identical, including eight cysteine residues.…”

Section: Molecular Genetics Of Gametophytic Self-incompatibilitymentioning

confidence: 99%

Gametophytic Self-Incompatibility Systems.

1993

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Self-incompatibility in Petunia inflata: isolation and characterization of cDNAs encoding three S-allele-associated proteins

Cited by 116 publications

References 16 publications

Evidence That Intragenic Recombination Contributes to Allelic Diversity of the S-RNase Gene at the Self-Incompatibility (S) Locus in Petunia inflata

Evidence That Intragenic Recombination Contributes to Allelic Diversity of the S-RNase Gene at the Self-Incompatibility (S) Locus in Petunia inflata

Identification and cDNA cloning for S-RNases in self-incompatible Japanese plum (Prunus salicina Lindl. cv. Sordum).

Gametophytic Self-Incompatibility Systems.

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