Self-microemulsifying drug delivery system and nanoemulsion for enhancing aqueous miscibility of Alpinia galanga oil

Khumpirapang, Nattakanwadee; Pikulkaew, Surachai; Müllertz, Anette; Okonogi, Siriporn

doi:10.1371/journal.pone.0188848

Cited by 18 publications

(12 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the different types of surfactant and co-surfactants also affected the ME region in the phase diagrams as well as the ratio of Smix [ 29 , 34 ]. Our previous work found that Tween 80 and ethanol at a weight ratio of 2:1 was the best ratio and was an appropriate Smix for AGO in providing the largest monophasic area or ME area in the phase diagrams [ 35 ]. Therefore, Tween 80 and ethanol at this ratio were used as Smix in the present study.…”

Section: Resultsmentioning

confidence: 99%

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

et al. 2021

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to develop a microemulsion (ME) containing Alpinia galanga oil (AGO), 1,8-cineole (C), or methyl eugenol (M) as an active pharmaceutical ingredient (API) for enhancing their antimicrobial activities. Agar diffusion, broth microdilution, and killing kinetics were used for antimicrobial evaluations. The ME composed of 30% API, 33.4% Tween 80, 16.6% ethanol, and 20% water appeared as translucent systems with droplet size and polydispersity index of 101.1 ± 1.3 nm and 0.3 ± 0.1, 80.9 ± 1.1 nm and 0.4 ± 0.1, and 96.6 ± 2.0 nm and 0.2 ± 0.1 for ME-AGO, ME-C, and ME-M, respectively. These ME formulations showed minimum bacterial concentrations of 3.91–31.25 µg/mL and 50% fungal inhibition concentrations of 1.83 ± 0.27–0.46 ± 0.13 µg/mL, 2–4 times stronger, and faster kinetic killing rate than their respective API alone. Keeping the ME formulations at 4 C, 25 C, and 40 C for 12 weeks did not affect their activities against fungi and Gram-negative bacteria, but the high temperature of 40 C decreased their activities against Gram-positive bacteria. It is concluded that ME is a promising delivery system for AGO and its major compounds to enhance their water miscibility and antimicrobial activities.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tween 80, and 70% w/w water was prepared according to a method previously described as it was reported that NE-AGO formulated by this method was chemically stable when stored at various temperatures (4°C, 20°C, and 40°C) for several weeks (0, 4, 8, 12 weeks) 7 . Briefly, the aqueous phase composed of Tween 80 and water was mixed using a vortex mixer for 5 min.…”

Section: Preparation and Characterization Of Ne-ago Ne-ago Composed Of 20%w/w Ago 10%w/wmentioning

confidence: 99%

“…A. galanga is well-known in Asian folk medicine and has been used for centuries as a food additive, an antimicrobial agent, a local anesthetic, an analgesic, and an antipruritic [3][4][5][6] . A. galanga oil (AGO) is rich in 1,8-cineole and 4-allylphenyl acetate 7 . AGO was recently shown to act as an anesthetic in fish 8 .…”

Section: Introductionmentioning

confidence: 99%

Binding effects of Alpinia galanga oil and its nanoemulsion 1 to GABAA receptors in rat cortical membranes

Khumpirapang

Suknuntha

Anuchapreeda

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Anesthetic activity of Alpinia galanga oil (AGO) has been reported however the mechanism of action in mammals has not been clear. In the present study, the binding effects of AGO and its three active components to gamma-aminobutyric acid type A (GABAA) receptor in cortical membranes of Sprague-Dawley rats were firstly investigated using a [3H]muscimol binding assay. Dimethyl sulfoxide (DMSO) was used to deliver these test samples. The results showed that only AGO and methyl eugenol displayed positive modulation at the highest concentration whereas 1,8-cineole and 4-allylphenyl acetate were inactive. An oil-in-water nanoemulsion containing 20%w/w AGO (NE-AGO) was formulated to deliver AGO instead of DMSO. This NE-AGO significantly enhanced a specific [3H]muscimol binding to 179% of the control with EC50 of 391 µg/mL. The result correlates well to the amount of methyl eugenol in AGO. This result confirms that the anesthetic activity of AGO and methyl eugenol is associated with GABAA receptor modulation, while that of 1,8-cineole and 4-allylphenyl acetate is not and may instead be related to other mechanisms. AGO showed well-tolerated by human cells. Therefore, the formulated NE-AGO might be a promising alternative anesthetic product for humans.

show abstract

“…Lipid-based drug delivery systems (LbDDS) can improve the apparent solubility, miscibility, and oral bioavailability of many poorly water-soluble drugs [ 11 , 12 ]. These systems are generally composed of oils, surfactants, and co-surfactants.…”

Section: Introductionmentioning

confidence: 99%

Development of Self-Nanoemulsifying Drug Delivery Systems Containing 4-Allylpyrocatechol for Treatment of Oral Infections Caused by Candida albicans

et al. 2021

Self Cite

View full text Add to dashboard Cite

Clinical use of 4-Allylpyrocatechol (APC), a potential antifungal agent from Piper betle, is limited because of its low water solubility. The current study explores the development of the self-nanoemulsifying drug delivery system (SNEDDS) containing APC (APC-SNEDDS) to enhance APC solubility. Results demonstrated that excipient type and concentration played an important role in the solubility of APC in the obtained SNEEDS. SNEDDS, comprising 20% Miglyol 812N, 30% Maisine 35-1, 40% Kolliphor RH40, and 10% absolute ethanol, provided the highest loading capacity and significantly increased water solubility of APC. Oil-in-water nanoemulsions (NE) with droplet sizes of less than 40 nm and a narrow size distribution were obtained after dispersing this APC-SNEDDS in water. The droplets had a negative zeta potential between −10 and −20 mV. The release kinetics of APC from APC-SNEDDS followed the Higuchi model. The NE containing 1.6 mg APC/mL had effective activity against Candida albicans with dose-dependent killing kinetics and was nontoxic to normal cells. The antifungal potential was similar to that of 1 mg nystatin/mL. These findings suggest that APC-SNEDDS are a useful system to enhance the apparent water solubility of APC and are a promising system for clinical treatment of oral infection caused by C. albicans.

show abstract

Self-microemulsifying drug delivery system and nanoemulsion for enhancing aqueous miscibility of Alpinia galanga oil

Cited by 18 publications

References 44 publications

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

Binding effects of Alpinia galanga oil and its nanoemulsion 1 to GABAA receptors in rat cortical membranes

Development of Self-Nanoemulsifying Drug Delivery Systems Containing 4-Allylpyrocatechol for Treatment of Oral Infections Caused by Candida albicans

Contact Info

Product

Resources

About