Self-Nanoemulsifying System in the Accumulation of Resveratrol and N- Acetylcysteine in the Epidermis and Dermis

Abstract

Trans -resveratrol (RES) and N-acetylcysteine (NAC) have protective effects on biological processes; therefore, they are frequently included in food supplements. Their possible applications for the prevention of free radical-induced damage to the skin are of particular physiological relevance; however, their usefulness is limited by their metabolic fate and the unpredictability of their delivery to the skin following oral administration. In this work, we evaluated the concomitant and direct application of RES and NAC on the skin using a self-nanoemulsifying system we previously developed for the oral delivery of poorly absorbed food supplements. We evaluated the capability of this system to increase RES and NAC accumulation in porcine skin using permeation studies in Franz diffusion cells. The ascorbyl palmitate (ASP) self-nanoemulsifying system considerably increased RES and NAC accumulation in the epidermis and dermis, which peaked 6 h after application. This study reveals a new formulation strategy to improve the bioavailability of ingredients, which was previously used in the health supplements field, but has rarely been employed in dermatology because of its poor distribution in the skin.

The Association of Oleic Acid and Dexamethasone Acetate into Nanocapsules Enables a Reduction in the Effective Corticosteroid Dose in a UVB Radiation-Induced Sunburn Model in Mice

“…After, the samples were filtered and analyzed using chromatography. For dermis and epidermis separation, the skin samples were immersed in a water bath at 60 °C for 45 s; the epidermis was removed with a spatula [ 37 , 38 ] and inserted into 1 mL of methanol. The remaining dermis was cut out and immersed in 2 mL of methanol.…”

A Systematic Review on Natural Antioxidant Properties of Resveratrol

“…Thousands of articles that describe the metabolism and bioactive proprieties of resveratrol and its close derivatives, were published in the last 20 years. In particular, the increasing interest shown in resveratrol is due to the discovery of its numerous biological activities and its beneficial effect on human health, providing protection from heart disease, cancer neuron damage, skin conditions and the effects of aging [20,[24][25][26][27][28][29][30][31][32][33][34][35][36][37][38].…”