In stable environments, cell size fluctuations are thought to be governed by simple 9 physical principles, as suggested by recent finding of scaling properties. Here we show, using E. coli, 10 that the scaling concept also rules cell size fluctuations under time-dependent conditions, even 11 though the distribution changes with time. We develop a microfluidic device for observing dense 12 and large bacterial populations, under uniform and switchable conditions. Triggering bacterial 13 reductive division by switching to non-nutritious medium, we find that the cell size distribution 14 changes in a specific manner that keeps its normalized form unchanged; in other words, scale 15 invariance holds. This finding is underpinned by simulations of a model based on cell growth and 16 intracellular replication. We also formulate the problem theoretically and propose a sufficient 17 condition for the scale invariance. Our results emphasize the importance of intrinsic cellular 18 replication processes in this problem, suggesting different distribution trends for bacteria and 19 eukaryotes. 20 21 Biswas et al. (2014a)), have been obtained under steady environments, for which our understanding 37 of single-cell growth statistics has also been significantly deepened recently (Ho et al. (2018); Jun 38 et al. (2018); Cadart et al. (2019)). By contrast, it is unclear whether such a simple concept as 39 1 of 25 Manuscript submitted to eLife scale invariance is valid under time-dependent conditions, where different regulations of cell cycle 40 kinetics may come into play in response to environmental variations. In particular, when bacterial 41 cells enter the stationary phase from the exponential growth phase, they undergo reductive division, 42 during which both the typical cell size and the amount of DNA per cell decrease (Nyström (2004); 43 Kaprelyants and Kell (1993); Arias et al. (2012); Gray et al. (2019)). Although this behavior itself is 44 commonly observed in test tube cultivation, little is known about single-cell statistical properties 45 during the transient. The bacterial reductive division is therefore an important model case for 46 studying cell size statistics under time-dependent environments and testing the robustness of the 47 scale invariance against environmental changes. 48 It has been, however, a challenge to observe large populations of bacteria under uniform yet 49 time-dependent growth conditions. For steady conditions, the Mother Machine (Wang et al. (2010)), 50 which allows for tracking of bacteria trapped in short narrow channels, was proved to be a powerful 51 tool for measuring cell size statistics. In such experiments, the channel width needs to be adapted 52 to cell widths in a given condition, and this renders the application to time-dependent conditions 53 difficult. If we enlarge the channels, depletion of nutrients in deeper regions of the channels 54 induces spatial heterogeneity, as discussed in ref.(Cho et al. (2007); Mather et al. (2010)) and later 55 in this article. Hence, it is als...