Background The aim of this methodological study was to assess the reliability and validity of the Sexual Quality of Life – Male (SQoL-M) questionnaire translated and adapted to measure the sexual quality of life of men in Turkey and Northern Cyprus. Methods: To assess the reliability and validity of the Turkish version of the SQoL-M questionnaire, in January and February 2017 we formed a sample of 128 men from Ankara, Turkey, and Girne, Northern Cyprus, to complete the questionnaire. Two weeks later, 65 of these men completed the questionnaire during a retest. Factor analysis was conducted to evaluate the questionnaire’s factor structure, internal consistency and test–retest reliability analyses were used to assess reliability and Pearson’s correlation coefficient was used to measure criterion-related validity. Results: Cronbach’s α for the Turkish version of the SQoL-M questionnaire was 0.91, with corrected item–total score correlations ranging from 0.432 to 0.819. The test–retest correlation, calculated to compare scale scores of both groups of participants, was 0.83 (P<0.001). We also observed a statistically significant relationship between the Turkish version of the SQoL-M questionnaire and the Arizona Sexual Experience scale. Conclusions: The Turkish version of the SQoL-M questionnaire is a valid, reliable instrument for evaluating the sexual quality of life of men in Turkey and Northern Cyprus.