Self-Sterilizing Materials. 2 . Evaluation of Surface Antibacterial Activity

Abstract

The cross-linked polyacrylamide films containing covalently-bonded N'-4-(2-acryloyloxyethyl)phenyl-N 5 -4-chlorophenylbiguanide hydrochloride (CPF-2 ) exhibit antibacterial activity at their surfaces and successfully act as selfsterilizing materials. Scanning electron microscopy studies indicate that inoculated cells of E . coli and S . aureus onto CPF-2 films undergo morphological changes, such as shrinkage and deformation, while those inoculated on blank cross-linked films remain intact. These morphological changes were ascribed to the collapse of the bacterial cells due to the action of released N 1 -4-(2-hydroxyethyl)phenyl-N 5 -4-chlorophenylbiguanide hydrochloride from CPF-2 by hydrolysis. INTRODUCTIOÑ

Antibacterial and Bacterium Adsorbing Macromolecules

“…[87,88,93] Therefore, the dead cells on the macromolecule surfaces might be killed by the leached substances and then adsorbed, or after being adsorbed they might be killed by the substances. Although many studies have been made on water-insoluble antibacterial polymers and macromolecules, with exception of those which liberate biocidal groups from, for example, these concerned with antibiotics, [25 -29] acriflavine (5), [32] ampicillin (6), [33] CPF-2, [42] 17, [43] TBZ (18), [44] AcDP (19), [45] PET films (35), [58] organotin (56,57), [72] etc., most authors of the studies have not investigated that substances might leach out of the macromolecules during the contact with cells in water. Studies on the leaching and on the prevention methods of that are expected.…”

“…Crosslinked polyacrylamide films [42] (CPF-2) containing covalently-bonded 16 c exhibit antibacterial activity at their surfaces and successfully act as self-sterilizing materials. SEM studies indicate that cells of E.coli and S.aureus inoculated onto CPF-2 films undergo morphological changes, such as shrinkage and deformation, while those inoculated on blank crosslinked films remain intact.…”

Preparation of Antibacterial Polymer-Grafted Silica Nanoparticle and Surface Properties of Composites Filled with the Silica (2)

“…7,8 On the other hand, various kinds of antibacterial polymers were reported. [9][10][11] For example, alkylphosphonium polymers show extremely strong antibacterial activity to Escherichia coli and Staphylococcus aureus. In the previous paper, we have succeeded in the grafting of polymer having alkylphosphonium sulfate groups (poly(St-SO 3 À P þ (Bu) 3 R)) onto the surface of silica nanoparticle.…”

Preparation of Antibacterial Polymer-grafted Nano-sized Silica and Surface Properties of Silicone Rubber Filled with the Silica

“…9,10 The grafting of polymers onto colloidal silica surface was also reported by Yoshinaga et al [11][12][13] On the other hand, various kinds of antibacterial polymer polymers were reported. [14][15][16][17] For example, alkyl phosphonium polymer shows extremely strong antibacterial nature to Escherichia coli and Staphylococcus aureus. It is expected that when antibacterial y To whom correspondence should be addressed (Tel&Fax: +81-25-262-6779, E-mail: ntsuboka@eng.niigata-u.ac.jp).…”