Semen cryopreservation in the Indian rhinoceros (Rhinoceros unicornis)

Stoops, Monica A.; Atkinson, Mark W.; Blumer, Evan S.; Campbell, Mark; Roth, Terri L.

doi:10.1016/j.theriogenology.2010.01.011

Cited by 32 publications

(23 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficiency of obtaining spermic ejaculates was 100%, but the ejaculate quality was highly variable. Although no information is available on tapirs, our data were comparable to previous reports on domestic stallions (Cary et al, 2004), the Przewalski's horse (Collins et al, 2006), and the rhinoceros (Hermes et al, 2005; Roth et al, 2005; Stoops et al, 2010), species taxonomically related to the tapir. Interestingly, unlike the horse and rhinoceros, Baird's tapir electroejaculates were not comprised of pre‐ejaculate and sperm‐rich fractions, facilitating the processing of the entire ejaculate.…”

Section: Discussionsupporting

confidence: 90%

“…The osmolality of semen was similar to that of the domestic horse (Love, 1992), the Przewalski's horse (Collins et al, 2006), and the rhinoceros (Roth et al, 2005; Stoops et al, 2010). Average pH of semen samples in this study was lower than those reported for the domestic stallion, Przewalski's horse (Collins et al, 2006), and the Indian rhinoceros (Stoops et al, 2010) and may be attributed to variations in contributions of the accessory glands in the tapir. The depth of anesthesia, as well as direct effects of the anesthetic drugs used, also may have compounded individual variation between tapirs.…”

Section: Discussionmentioning

confidence: 61%

“…Spermatozoa have been successfully cryopreserved in numerous nondomestic species (Monfort et al, 1993; Saragusty et al, 2006; Hermes et al, 2007; Garde et al, 2008; Hermes et al, 2009; Stoops et al, 2010). Interestingly, most cryodiluents are egg yolk or skim‐milk based, contain glycerol or dimethylsulfoxide, and have yielded variable results.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)

Pukazhenthi

Togna

Padilla

et al. 2011

Journal of Andrology

View full text Add to dashboard Cite

There is little information on the reproductive biology of the male Baird's tapir (Tapirus bairdii). In this study, we characterized the ejaculate traits and evaluated the efficacy of 2 cryodiluents on sperm cryosurvival. Ejaculates were assessed for volume, pH, sperm motility, forward progression, osmolality, sperm concentration, sperm morphology, and acrosomal integrity. For cryopreservation, ejaculates with .50% total sperm motility were washed, and sperm pellets were resuspended in either Botu-Crio (CryoVital, Grandau, Germany) or INRA 96 containing 2% egg yolk and 2.5% each of methyl-and dimethylformamide (INRA 96), and they were cryopreserved over liquid nitrogen vapor. Thawed samples were incubated in vitro (25uC) and evaluated for percent total sperm motility, forward progression, and acrosomal integrity at hourly intervals for 4 hours. Spermic ejaculates were obtained from all males, and the mean seminal volume, sperm concentration per milliliter, percent sperm motility, progressive status, and percent morphologically normal cells were 20.4 6 4.3 mL, 101.2 6 24.0 6 10 6 /mL, 46.1% 6 5.0%, 2.9 6 0.1, and 6.9% 6 1.4%, respectively. There was a positive significant correlation between percent normal sperm and animal age (r 5 0.66; P , .004). Cryopreservation in either Botu-Crio or INRA 96 resulted in a decline (P , .05) in percent sperm motility and acrosomal integrity. Sperm forward progression remained unaffected immediately after thawing in INRA 96 but continued to decline over time. These results characterize, for the first time, the ejaculate traits of the tapir; demonstrate that tapir spermatozoa can be cryopreserved in diluents containing amides alone or in combination with glycerol; and provide fundamental information critical for development of assisted reproductive technologies for the Baird's tapir.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 61%

See 1 more Smart Citation

Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)

Pukazhenthi

Togna

Padilla

et al. 2011

Journal of Andrology

View full text Add to dashboard Cite

show abstract

“…For example, the use of glycerol has allowed the recovery of spermatozoa after thawing in diverse species and at similar volume-to-volume concentrations (4%–8%), ranging from various felid species (Crosier et al 2006; Stoops et al 2007) to marine mammals (Robeck and O’Brien 2004; Robeck et al 2011) to the Asian elephant ( Elephas maximus ; Saragusty et al 2009; Thongtip et al 2009). More recently, spermatozoa from the Przewalski’s horse ( Equus ferus przewalskii ; Pukazhenthi et al 2010), Baird’s tapir ( Tapirus bairdii ; Pukazhenthi et al 2011) and Indian rhinoceros ( Rhinoceros unicornis ; Stoops et al 2010) have been found to respond well to cryodilution and freezing protocols originally developed for the domestic horse (all members of Perissodactyla). In contrast are the wildebeest ( Connochaetes taurunus ) and greater kudu ( Tragelaphus strepsiceros ), which produce more sensitive spermatozoa that can only tolerate 2.0%–2.5% glycerol (Schiewe et al 1991), generally only half that commonly used in livestock.…”

Section: Examples In Comparative Seminal Traits and Cryosensitivitymentioning

confidence: 99%

Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options

Comizzoli

Wildt

2014

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Human-related fertility preservation strategies have enormous potential for helping sustain and protect other species, especially to assist managing or ‘rescuing’ the genomes of genetically valuable individuals, including endangered species. However, wider-scale applications are limited by significant physiological variations among species, as well as a lack of fundamental knowledge of basic reproductive traits and cryosensitivity. Systematic and comparative cryopreservation studies (e.g. on membrane biophysical properties and resilience to freezing temperatures) are required to successfully recover gametes and gonadal tissues after thawing and eventually produce healthy offspring. Such data are currently available for humans and a few laboratory and livestock animals, with virtually all other species, including wildlife, having gone unstudied. Interestingly, there also are commonalities among taxa that allow a protocol developed for one species to provide useful information or guidance for another. However, when a rare animal unexpectedly dies there is no time for a prospective understanding of that species’ biophysical traits. Because the odds of success will be much lower in such instances, it is essential that more fundamental studies be directed at more species. But also worthwhile is thinking beyond these systematic characterisations to consider the potential of a ‘universal preservation protocol’ for animal biomaterials.

show abstract

“…Unfortunately, the post-thaw quality of cryopreserved Asian elephant semen proved to be very low, and relatively few bulls were capable of providing semen able to adequately survive the freezing process (Graham et al, 2004;Thongtip et al, 2004;Sa-Ardrit et al, 2006;Saragusty et al, 2009). While post-thaw semen quality depends on a variety of factors, fresh semen quality is used as one of the primary indicators of suitability for semen cryopreservation in many species; in particular, the percentage of motile sperm is a widely used criterion for selecting semen samples prior to freezing (Isachenko et al, 2004;Saragusty et al, 2009;Akhter et al, 2010;Stoops et al, 2010). In some species (e.g.…”

Section: Introductionmentioning

confidence: 99%

Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen

et al. 2013

View full text Add to dashboard Cite

Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to compare the influence of extender (TEST versus INRA96 ® ) and penetrating cryoprotectants (3% glycerol, 5% glycerol and 4% methylformamide) on postthaw semen quality. We demonstrate that not only the freezing process, but also the quality of the semen before freezing, significantly influences the freezability of Asian elephant semen. Prefreeze motility, viability, semen volume, semen pH, sperm concentration and the incidence of sperm mid-piece and tail abnormalities all significantly (p<0.05) affected post-thaw semen quality. While extender and cryoprotectant did not significantly affect any of the above semen quality parameters post-thaw, the skim-milk based extender (INRA96 ® ) preserved DNA integrity better (p<0.05) than the egg yolk extender (TEST). Considerable between-ejaculate variation in all postthaw semen quality parameters was also noted. It is concluded that strict criteria for semen quality is essential for the selection of Asian elephant bull ejaculates suitable for cryopreservation; stricter initial selection should improve the mean post-thaw quality.

show abstract

Semen cryopreservation in the Indian rhinoceros (Rhinoceros unicornis)

Cited by 32 publications

References 36 publications

Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)

Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)

Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options

Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen

Contact Info

Product

Resources

About