Semen parameters and seminal plasma protein and biochemical profiles of dogs with benign prostatic hyperplasia after botulinum toxin type A intraprostatic injection

Abstract

This study aimed to determine the effects of different concentrations of botulinum toxin type A (BT-A) on semen parameters, and seminal plasma biochemical and protein profi les of dogs with benign prostatic hyperplasia (BPH). RESUMO O objetivo do presente estudo foi determinar a ação de diferentes concentrações de toxina botulínica tipo A (TB-A) sobre os parâmetros seminais, perfi s bioquímicos e proteicos do plasma seminal de cães com hiperplasia prostática benigna (HPB). Dezoito cães hígidos, não orquiectomizados com HPB foram divididos em três grupos, os quais foram submetidos à injeção intra-prostática de solução salina (grupo controle -GC), 250UI (GI) ou 500UI (GII) de TB-A. Amostras seminais foram coletadas previamente aos tratamentos e após 2, 4 e 8 semanas. Os parâmetros seminais assim como os valores de pH e concentrações de proteínas totais (TP), cloretos totais (CT), cálcio (Ca), potássio (K), sódio (Na

Semen Characteristics in German Shepherd Dogs

“…The total protein level was 2.98 ± 0.23 g/dl for GSD. The total protein concentration in the seminal plasma of mixed breeds (GSD and Rottweiler) was between 1.88 to 2.3 g/dl (Aquino cortez, 2003 andMotheo, 2014) which was in close agreement with the present study. The albumin level in seminal plasma was between 0.16 ± 0.03 to 0.66 ± 0.19 g/dl in GSD.…”

Serum testosterone, sperm quality, cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly

“…Abdominal ultrasound has been routinely used to diagnose prostate diseases (Flores et al., ; Motheo et al., ; Polisca et al., ), and the haemospermia is a common clinical signs in animals with prostate diseases, especially in cases of BPH (Smith, ; Zambelli et al., ). These two analyses allowed the adequate allocation of dogs in the experimental groups.…”

“…Among the prostate diseases, benign prostatic hyperplasia (BPH) is the most frequent (Polisca, Troisi, Fontaine, Menchetti, & Fontbonne, ). Although prostate biopsy is considered the most suitable method for the specific diagnosis of the canine prostate diseases (Motheo et al., ), there are limitations and complications as the older dogs sedation, haemorrhage (Kustritz, ), peritonitis or spread of neoplastic cells (Smith, ). Thus, more research is needed to make possible the use of techniques less invasive, rapid, inexpensive and efficient for an early diagnosis of prostatic diseases.…”

“…In dog, prostate is the only accessory sex gland, its fluid constitutes approximately 95% of the ejaculate (Iguer‐Ouada & Verstegen, ), and it is possible that prostate diseases can cause significant biochemical changes in the seminal plasma. However, few studies quantified the biochemical constituents in seminal plasma of healthy dogs (England & Allen, ; Mann & L‐Mann, ) or dogs with prostatic diseases (Motheo et al., ). The biochemical assessment of the canine prostatic fluid may help to identify possible biomarkers of prostatic diseases and may provide important benefits for human medicine (Aquino‐Cortez, Silva, Araújo, Menezes, & Moura, ), as the dog is used as an experimental model for the study of prostatic diseases in men (Leoci, Aiudi, Silvestre, Lissner, & Lacalandra, ).…”

Proteins of the canine seminal plasma

“…The concentrations of the proteins of the canine SP range from 1.88g dL -1 (AQUINO-CORTEZ, 2003) to 2.3g dL -1 (MOTHEO et al, 2014). Higher protein concentrations are present in the second fraction of the ejaculate (rich in spermatozoa, 4.15g dL -1 ) than in the third fraction or prostatic fluid (2.00g dL -1 , BARTLETT, 1962).…”