Semi-automated and efficient parallel SELEX of aptamers for multiple targets

Jiang, Meng; Fang, Xiaona; Diao, Han; Lv, Shaokang; Zhang, Zheng; Zhang, Xiang; Chen, Zhiwei; Luo, Zhaofeng

doi:10.1039/d3ay00367a

Search citation statements

Order By: Relevance

Paper Sections

Select...

Results1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2023

2024

Publication Types

Select...

Article4

Preprint1

Relationship

Self Cite1

Independent4

Authors

Journals

Cited by 6 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MB-SELEX screening process comprises three main processes 27 as follows: (1) the incubation of the initial ssDNA library with magnetic bead-coupled proteins, (2) the removal of unbound sequences through washing, and (3) the elution of bound sequences from the beads (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

Liu,

Jiang,

et al. 2024

Anal. Methods

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

A rapid and sensitive aptamer-based biosensor for beta-lactoglobulin in milk

Liu,

Jiang,

et al. 2024

Anal. Methods

Self Cite

View full text Add to dashboard Cite

show abstract

Development of an Automated Capture-SELEX Device for Efficient Screening of β-Conglycinin Aptamer

Zhang,

Ning

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

β-Conglycinin is the main allergen present in soybeans, and it is causing wide concern due to its notable allergenicity, heat, and digestive enzyme resistance. Screening for aptamers that both recognize β-conglycinin and inhibit the allergic reactions that it triggers is necessary. Conventional aptamer screening is labor-intensive, requires skilled personnel, and has limited reproducibility. To address these limitations, an automated device was developed to enhance the efficiency of aptamer selection in Capture-SELEX. The device achieves highly integrated, reproducible, and accurate contamination control. Using this device, a high-affinity and specific aptamer, β-5, was selected with a K d = 18.24 ± 2.42 nM for β-conglycinin, as confirmed by isothermal titration calorimetry and fluorescence polarization. Thermodynamic analysis revealed that enthalpy-driven binding and docking simulations clarified the recognition mechanism. Overall, this automated device enables high-efficiency aptamer generation for certain targets, with aptamer β-5 expected to play a vital role in the detection of β-conglycinin and the targeted inhibition of its allergic reaction.

show abstract