Optical image analysis (OIA) supporting microscopic observation can be applied to improve ore mineral characterization of ore deposits, providing accurate and representative numerical support to petrographic studies, on the polished section scale. In this paper, we present an experimental application of an automated mineral quantification process on polished sections from Zaruma-Portovelo intermediate sulfidation epithermal deposit (Ecuador) using multispectral and color images. Minerals under study were gold, sphalerite, chalcopyrite, galena, pyrite, pyrrhotite, bornite, hematite, chalcocite, pentlandite, covellite, tetrahedrite and native bismuth. The aim of the study was to quantify the ore minerals visible in polished section through OIA and, mainly, to show a detailed description of the methodology implemented. Automated ore identification and determination of geometric parameters predictive of geometallurgical behavior, such as grade, grain size or liberation, have been successfully performed. The results show that automated identification and quantification of ore mineral images are possible through multispectral and color image analysis. Therefore, the optical image analysis method could be a consistent automated mineralogical alternative to carry on detailed ore petrography.Geosciences 2016, 6, 30 2 of 23 with important contributions in metallurgical processes [13][14][15] and provide rapid, statistically reliable and repeatable mineralogical, petrographic and metallurgical data.OIA is a convenient, accessible, and inexpensive tool for obtaining comprehensive information about fine fractions of the ore [13][14][15][16]. However, a limiting factor in direct OIA is the discrimination between minerals with similar reflective properties [17]. A digital image is a numerical representation of a two-dimensional image. Digital cameras used in optical microscopy usually incorporate a charge-coupled device (CCD) to capture images. The CCD transfers the optical photon data received through a filter into electronic pulse or photo. The generated voltage is then converted into pixels (converting analogical data to digital data) and stored as a digital image that contains a fixed number of rows and columns of pixels [17,18]. A color image is a digital image that includes color information for each pixel. Color images can be acquired using a CCD digital camera (i.e., 3CCD camera, Bayer mosaic filter or three shot color sampling). For visually acceptable digital color images, it is necessary to provide grey levels (GL) in three bands/channels for each pixel, which are interpreted as coordinates in one of the existing color spaces.The Red, Green and Blue (RGB) color model is an additive color model in which red, green, and blue light is added together in various ways to reproduce a broad array of colors. It is commonly applied in computer displays but other models such as hue-saturation-value (HSV) are also in use. RGB images have been employed for the identification and quantification of opaque minerals in polished secti...