Semi-interpenetrating nanosilver doped polysaccharide hydrogel scaffolds for cutaneous wound healing

Preethi, G. U.; Unnikrishnan, B.S.; Sreekutty, J; Archana, M.G.; Anupama, M.S.; Shiji, R.; Pillai, Kamala; Joseph, Manu M.; Syama, H.P.; Sreelekha, T.T.

doi:10.1016/j.ijbiomac.2019.10.012

Cited by 33 publications

(27 citation statements)

References 31 publications

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“…Hydrogel preparation using PST001 and acrylamide has been previously established and well-characterized. 15 The application of hydrogels for wound healing has been established. It was observed that an interpenetrating network formed between natural and synthetic polymers showed excellent swelling ability.…”

Section: Discussionmentioning

confidence: 99%

“…PST001 based composite hydrogel was prepared using a previously established protocol and detailed characterization was reported previously by the authors. 15 Figure 2(a) represents the scanning electron microscopic image of the hydrogel at a magnification of 500X showing smooth surface topography which will be beneficial for cell attachment and proliferation. The absorption, distribution, metabolism, excretion, and toxicity was determined using the pkCSM bioinformatics tool and revealed that the hydrogel had no severe toxicity to human or environment, showing better compatibility compared with acrylamide gels without polysaccharide (Figure S2).…”

Section: Polysaccharide Based Hydrogel Formation Confirmedmentioning

confidence: 99%

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Engineering cartilage graft using mesenchymal stem cell laden polyacrylamide-galactoxyloglucan hydrogel for transplantation

et al. 2021

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Hydrogels are reported to have various biomedical field applications, and many reports also suggest that soft gels promote stem cell differentiation. Chondrogenic differentiation of mesenchymal stem cells (MSC) is significant in articular cartilage repair. This study focuses on polysaccharide-based hydrogels which enhance chondrocyte lineage differentiation of MSC when grown in the hydrogels. This study implies that the prepared hydrogels promote specific lineage without any external chemical induction factors. The techniques, including immunofluorescence and functional assays to assess the differentiation and in vivo implantation, were employed. All observations paved the way towards confirmation that the galactoxyloglucan-based hydrogel is an attractive candidate for supporting stem cell growth and cartilaginous differentiation.

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Section: Discussionmentioning

confidence: 99%

Section: Polysaccharide Based Hydrogel Formation Confirmedmentioning

confidence: 99%

Engineering cartilage graft using mesenchymal stem cell laden polyacrylamide-galactoxyloglucan hydrogel for transplantation

et al. 2021

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“…To prolong the action time of cytokines on the wound surface, we formed a semi-solid drug reservoir through the alliance of adipose-derived stem cell concentrated conditioned medium (ADSCC-CM) and the polysaccharide hydrogel aiming to exert its sustained-release effect. Currently, polysaccharide hydrogel has emerged as a scaffold material with good biocompatibility while achieving widespread adoption into the clinical realm by virtue of its tunable morphology, controllable degradation, and release behaviour [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Combination of lyophilized adipose-derived stem cell concentrated conditioned medium and polysaccharide hydrogel in the inhibition of hypertrophic scarring

et al. 2021

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Background Mesenchymal stem cell-based acellular therapies have been widely exploited in managing hypertrophic scars. However, low maintenance dose and transitory therapeutic effects during topical medication remain a thorny issue. Herein, this study aimed to optimize the curative effect of adipose-derived stem cell conditioned medium (ADSC-CM) in the prevention of hypertrophic scarring. Methods In the present study, ADSC-CM was concentrated via the freeze-drying procedure. The efficacy of different dose groups (CM, CM5, CM10) was conducted on the proliferation, apoptosis, and α-smooth muscle actin (α-SMA) expression of human keloid fibroblasts (HKFs) in vitro. Incorporation of adipose-derived stem cell concentrated conditioned medium (ADSCC-CM) into polysaccharide hydrogel was investigated in rabbit ear, in vivo. Haematoxylin-eosin (H&E) and Masson’s trichrome staining were performed for the evaluation of scar hyperplasia. Results We noted that ADSCC-CM could downregulate the α-SMA expression of HKFs in a dose-dependent manner. In the rabbit ear model, the scar hyperplasia in the medium-dose group (CM5) and high-dose group (CM10) was inhibited with reduced scar elevation index (SEI) under 4 months of observation. It is noteworthy that the union of CM5 and polysaccharide hydrogel (CM5+H) yielded the best preventive effect on scar hyperplasia. Briefly, melanin, height, vascularity, and pliability in the CM5+H group were better than those of the control group. Collagen was evenly distributed, and skin appendages could be regenerated. Conclusions Altogether, ADSCC-CM can downregulate the expression of α-SMA due to its anti-fibrosis effect and promote the rearrangement of collagen fibres, which is integral to scar precaution. The in situ cross bonding of ADSCC-CM and polysaccharide hydrogel could remarkably enhance the therapeutic outcomes in inhibiting scar proliferation. Hence, the alliance of ADSCC-CM and hydrogel may become a potential alternative in hypertrophic scar prophylaxis.

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“…AgNPs are more toxic compared to bulk silver but they have a strong anti-inflammatory impact during tissue healing and can be integrated into composite materials to obtain antibacterial properties [29][30][31]. The human dietary intake of silver, owing to the widespread use of silver compounds, is estimated at 70-90 µg per day [32].…”

Section: Introductionmentioning

confidence: 99%

In Situ and Ex Situ Designed Hydroxyapatite: Bacterial Cellulose Materials with Biomedical Applications

et al. 2020

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Hydroxyapatite (HAp) and bacterial cellulose (BC) composite materials represent a promising approach for tissue engineering due to their excellent biocompatibility and bioactivity. This paper presents the synthesis and characterization of two types of materials based on HAp and BC, with antibacterial properties provided by silver nanoparticles (AgNPs). The composite materials were obtained following two routes: (1) HAp was obtained in situ directly in the BC matrix containing different amounts of AgNPs by the coprecipitation method, and (2) HAp was first obtained separately using the coprecipitation method, then combined with BC containing different amounts of AgNPs by ultrasound exposure. The obtained materials were characterized by means of XRD, SEM, and FT-IR, while their antimicrobial effect was evaluated against Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and yeast (Candida albicans). The results demonstrated that the obtained composite materials were characterized by a homogenous porous structure and high water absorption capacity (more than 1000% w/w). These materials also possessed low degradation rates (<5% in simulated body fluid (SBF) at 37 °C) and considerable antimicrobial effect due to silver nanoparticles (10–70 nm) embedded in the polymer matrix. These properties could be finetuned by adjusting the content of AgNPs and the synthesis route. The samples prepared using the in situ route had a wider porosity range and better homogeneity.

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Semi-interpenetrating nanosilver doped polysaccharide hydrogel scaffolds for cutaneous wound healing

Cited by 33 publications

References 31 publications

Engineering cartilage graft using mesenchymal stem cell laden polyacrylamide-galactoxyloglucan hydrogel for transplantation

Engineering cartilage graft using mesenchymal stem cell laden polyacrylamide-galactoxyloglucan hydrogel for transplantation

Combination of lyophilized adipose-derived stem cell concentrated conditioned medium and polysaccharide hydrogel in the inhibition of hypertrophic scarring

In Situ and Ex Situ Designed Hydroxyapatite: Bacterial Cellulose Materials with Biomedical Applications

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