Semi-interpenetrating polymer networks (IPNs) for entrapment of glucose isomerase

Preparation and application of poly(N,N-dimethylacrylamide-co-acrylamide) and poly(N-isopropylacrylamide-co-acrylamide)/κ-Carrageenan hydrogels for immobilization of lipase

“…In this method, enzymes do not chemically bond to polymeric matrices. So, the three-dimensional structure of the enzymes may not be affected upon immobilization procedure and thus the optimum parameters (pH and temperature) of both the immobilized and free form may be identical [11,12]. The optimum temperature of covalently immobilized lipase, although, was shifted to 35 • C. This was either due to the creation of conformational limitation on the enzyme movement as a result of covalent bond formation between the enzyme and the support or a low restriction in the diffusion of the substrate at high temperature [8,12,15,17].…”

“…Over the last five decades, various methods, including adsorbing enzymes into a porous support by physical adsorption [6], covalently attaching enzymes onto an activated surface [7,8], cross-linking enzymes into crystals [9], and entrapping enzymes into a polymer matrix [10][11][12] have been explored to immobilize the biocatalysts. Since enzymes are a group of very diverse biological entities, there is no universal method for enzyme immobilization.…”

Industrial Enzyme Applications in Biorefineries for Starchy Materials

“…GI obtained from different sources such as Flavobacterium , Bacillus , and some Streptomyces and Arthrobacter species is immobilized on different support materials such as DEAE cellulose (Chen and Anderson 1979 ;Huitron and Limon-Lason 1978 ), polyacrylamide gel (Demirel et al 2006 ;Strandberg and Smiley 1971 ), and alginate beads (Rhimi et al 2007 ).…”

Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

“…Of the various immobilization methods, entrapment of enzymes within a hydrogel matrix has attracted the most attention [3][4][5][6][7]. Hydrogels are crosslinked polymers with a three-dimensional network arrangement and are able to retain the large amounts of water needed for enzyme activity.…”