Semi-Reversible Cross-Linking. Synthesis and Application of a Novel Heterobifunctional Reagent

Friebel, Klaus; Huth, Helga; Jany, K.-D.; Trommer, Wolfgang E.

doi:10.1515/bchm2.1981.362.1.421

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1984

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Cited by 7 publications

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“…This may be accomplished by disulfide exchange or by a substitution reaction with thiosulfonates (). Cross-linking reagents with sulfhydryl-specific functionalities and additional photoreactive groups have been described ( − ).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Application of Novel Bifunctional Spin Labels

et al. 1999

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The synthesis of new bifunctional spin-labeled cross-linking reagents is described. Covalent attachment to papain was achieved via a thiol-specific thiosulfonate residue and, for the second anchor point, via a nonspecific photoreactive azido function. The thiosulfonate formed a reversible disulfide linkage, which could be cleaved again reductively by dithiothreitol. The spin label, a pyrroline-1-oxyl radical, was highly immobilized after attachment to papain by both functional groups and showed little if any relative motion with respect to the protein.

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