Semiautomated Segmentation and Measurement of Cytoplasmic Vacuoles in a Neutrophil With General‐Purpose Image Analysis Software

Mizukami, Maki; Yamada, Misaki; Fukui, Sayaka; Fujimoto, Nao; Yoshida, Shigeru; Kaga, Sanae; Obata, Keiko; Jin, Shigeki; Miwa, Keiko; Masauzi, Nobuo

doi:10.1002/jcla.21957

Cited by 4 publications

(2 citation statements)

References 8 publications

(7 reference statements)

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“…Photoshop ® CS6 (Adobe System Inc., San Jose, CA) (PS) and Image‐J (Ver.1.43, available at rsbweb.nih.gov/ij; accessed April 14, 2014) (IJ), hereafter called the “PS‐IJ method”, were used for image processing. The details of the original “PS‐IJ method” are described elsewhere . We developed a modified version of the PS‐IJ method for NAP rating called the “NAP‐PS‐IJ”.…”

Section: Methodsmentioning

confidence: 99%

Semi‐Automatic Rating Method for Neutrophil Alkaline Phosphatase Activity

Sugano¹,

Hashi²,

Goto

et al. 2016

Clinical Laboratory Analysis

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Section: Methodsmentioning

confidence: 99%

Semi‐Automatic Rating Method for Neutrophil Alkaline Phosphatase Activity

Sugano¹,

Hashi²,

Goto

et al. 2016

Clinical Laboratory Analysis

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“…The quantification of the nephrin-positive area was performed according to the protocol described by Mizukami et al with minor modifications (Mizukami et al 2016). Briefly, immunofluorescence microscopic images were transferred into Photoshop software (v 22.3.1, Adobe Systems Inc, San Jose, CA, USA).…”

Section: Confocal Immunofluorescence Microscopy and Electron Microscopymentioning

confidence: 99%

Dynamic changes of podocytes caused by fibroblast growth factor 2 in culture

et al. 2021

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Fibroblast growth factor 2 (FGF2) augments podocyte injury, which induces glomerulosclerosis, although the mechanisms remain obscure. In this study, we investigated the effects of FGF2 on cultured podocytes with interdigitating cell processes in rats. After 48 h incubation with FGF2 dynamic changes in the shape of primary processes and cell bodies of podocytes resulted in the loss of interdigitation, which was clearly shown by time-lapse photography. FGF2 reduced the gene expressions of constituents of the slit diaphragm, inflections of intercellular junctions positive for nephrin, and the width of the intercellular space. Immunostaining for the proliferation marker Ki-67 was rarely seen and weakly stained in the control without FGF2, whereas intensely stained cells were frequently found in the presence of FGF2. Binucleation and cell division were also observed, although no significant increase in cell number was shown. An in vitro scratch assay revealed that FGF2 enhanced migration of podocytes. These findings show that FGF2 makes podocytes to transition from the quiescent state into the cell cycle and change their morphology due to enhanced motility, and that the culture system in this study is useful for analyzing the pathological changes of podocytes in vivo.

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