PS-IJ method can successfully segment vacuoles and measure the rAV and rECA, becoming a useful tool for quantitative description of morphological observation of blood and marrow film.

Near-monodispersed micrometer-sized polystyrene (PS) particles carrying amidino and carboxyl groups on their surfaces were synthesized by soap-free emulsion polymerization using an amphoteric free radical initiator. The resulting amphoteric PS particles were characterized in terms of diameter, morphology, disperibility in aqueous media and surface charge using scanning electron microscopy (SEM), optical microscopy (OM), sedimentation rate and electrophoretic measurements. At pH 2.0, where the amidino groups are protonated (positively charged), and at pH 11.0, where the carboxyl groups are deprotonated (negatively charged), the PS particles were well dispersed in aqueous media via electrostatic repulsion. At pH 4.8, where the surface charges are neutral, the PS particles were weakly aggregated. Furthermore, it was confirmed that the PS particles can function as a pH-sensitive foam stabilizer: foamability and foam stability were higher at pH 2.0 and 4.8, where the PS particles can be adsorbed to the air–water interface, and lower at pH 11.0, where the PS particles tend to disperse in bulk aqueous medium. SEM and OM studies indicated that hexagonally close-packed arrays of PS particles were formed on the bubble surfaces and moiré patterns were observed on the dried foams. Moreover, the fragments of dried foams showed iridescent character under white light.

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