Seminal BAX and BCL2 Gene and Protein Expressions in Infertile Men With Varicocele

Mostafa, Taymour; Rashed, Laila Ahmed; Nabil, Nashaat; Amin, Rania A.

doi:10.1016/j.urology.2014.05.016

Cited by 44 publications

(42 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many pathways result in testicular apoptosis, and these processes appear to be regulated by the plasma membrane externalisation of negatively charged membrane phosphatidylserine, mitochondrial dysfunction, chromatin condensation and DNA fragmentation (Ku, Shim, Kim, & Paick, ; Mostafa, Rashed, Nabil, & Amin, ). Granulysin structure suggests a potential mechanism of action; the positive charges of granulysin appear to attract the molecule towards the negatively charged surface cells (Smyth et al., ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of seminal granulysin in infertile men with varicocele

et al. 2018

Self Cite

View full text Add to dashboard Cite

Varicocele has a common association with male infertility, but its exact role is still debated. Apoptosis has been suggested as one of the mechanisms of varicocele-associated infertility. Granulysin is a molecule that plays a role in apoptosis with no previous study about its role in male infertility. This case-controlled study aimed to assess seminal plasma granulysin level in infertile patients with varicocele. This study involved 90 men that were allocated into fertile normozoospermic men (n = 20), infertile men without varicocele (n = 30) and infertile men with varicocele (n = 40). These men were subjected to history taking, clinical examination, semen analysis and estimation of seminal granulysin. In general, seminal granulysin level was significantly elevated in infertile men compared with fertile men. Infertile men with varicocele showed significantly higher seminal granulysin compared with infertile men without varicocele, in bilateral varicocele cases and in grade III varicocele. Seminal granulysin level was negatively correlated with sperm concentration, sperm motility, sperm normal forms percentage and testicular volumes. It is concluded that increased seminal granulysin has a negative impact on spermatogenesis in infertile men in general and in infertile men associated with varicocele in particular.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of seminal granulysin in infertile men with varicocele

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cocuzza et al (2012) pointed that as Vx grade increased, seminal reactive oxygen species increases and sperm concentration decreases. Mostafa et al (2012Mostafa et al ( , 2014 pointed that seminal OS is related to increased Vx grade in infertile OAT men associated with Vx.…”

Section: Discussionmentioning

confidence: 99%

Seminal cyclooxygenase relationship with oxidative stress in infertile oligoasthenoteratozoospermic men with varicocele

Mostafa

Rashed

Taymour³

2015

Andrologia

View full text Add to dashboard Cite

This study aimed to assess the relation of seminal cyclooxygenase COX-1, COX-2 with oxidative stress in infertile oligoasthenoteratozoospermic (OAT) men with varicocele (Vx). In all, 128 men were allocated into fertile men, fertile men with Vx, infertile OAT men without Vx and infertile OAT men with Vx. They were subjected to history taking, clinical examination and semen analysis. Also, seminal COX-1, COX-2, malondialdehyde (MDA) and glutathione peroxidase (GPx) were estimated. Mean levels of seminal COX-1, COX-2 were over-expressed, the mean level of seminal MDA was significantly increased, and the mean level of seminal GPx was significantly decreased in infertile OAT men with Vx compared with other groups. Seminal COX-1 and COX-2 were over-expressed in cases with Vx grade III compared with Vx grades I, II cases and in cases with bilateral Vx compared with unilateral Vx. There was significant negative correlation between seminal COX-1 and COX-2 with sperm concentration, sperm motility, sperm normal morphology, seminal GPx and significant positive correlation with seminal MDA. It is concluded that seminal COX-1 and COX-2 are over-expressed in infertile OAT men with Vx compared with fertile men with/without and infertile OAT men without Vx being associated with oxidative stress, Vx grade and Vx laterality.

show abstract

“…In fact, various molecular mechanisms have been proposed for elucidating apoptosis in germ cells. The study in the Bcl‐2 apoptotic family indicated that seminal Bcl‐2 demonstrated a positive correlation with sperm concentration and sperm motility in infertile men with varicocele, whereas Bax demonstrated a negative correlation (Mostafa, Rashed, Nabil, & Amin, ). Nuclear factor‐kappa B (NF‐κB), a key regulator of the inflammatory gene, was reported to be involved in varicocele‐induced testicular dysfunction (Tuğcu et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Depending on the character of the 14‐3‐3 protein, analysing the binding partners of 14‐3‐3ε may help reveal the mechanism of germ cell apoptosis. Some notable studies have suggested that Bcl‐2 family proteins play an important role during germ cell apoptosis in rats and humans (Lee, Lee, Cheng, & Jeng, ; Mostafa et al, ). 14‐3‐3 protein protected cell apoptosis by binding pro‐apoptotic proteins such as Bax and Bad (Nomura et al, ; Rapp, Fischer, Rennefahrt, Hekman, & Albert, ).…”

Section: Discussionmentioning

confidence: 99%

14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

Liu

et al. 2019

Andrologia

View full text Add to dashboard Cite

The latest perspective indicates that apoptotic dysregulation is an important mechanism in male infertility induced by varicocele. To elucidate the molecular mechanism of apoptosis caused by varicocele, we used proteomics (2D-MALDI-TOF MS) to identify the altered proteins in the testes of experimental varicocele rats compared with the control. Here, 21 significantly different protein spots were detected by proteomics technology. 14-3-3 epsilon (14-3-3ε) was our subsequent research target because of its function in apoptosis. The expression of 14-3-3ε in rat testes was confirmed by Western blot and immunohistochemistry, and the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) method was used to analyse the apoptosis of germ cells. GC-1 spg cells transfected with small interfering RNA were used to confirm the function of 14-3-3ε in vitro. 14-3-3ε protein expression decreased, accompanied by a higher apoptosis index in rat testes of the varicocele group. Furthermore, 14-3-3ε siRNA-treated GC-1 spg cells caused the upregulation of the apoptotic rate detected by flow cytometry. The expression of Bax and Bcl-2 was found to be regulated by 14-3-3ε in vitro. Our investigation demonstrated the pro-apoptotic function of the downregulation of 14-3-3ε, which may play an important role in germ cell apoptosis induced by varicocele.

show abstract

Seminal BAX and BCL2 Gene and Protein Expressions in Infertile Men With Varicocele

Cited by 44 publications

References 18 publications

Assessment of seminal granulysin in infertile men with varicocele

Assessment of seminal granulysin in infertile men with varicocele

Seminal cyclooxygenase relationship with oxidative stress in infertile oligoasthenoteratozoospermic men with varicocele

14‐3‐3 epsilon plays an important role in testicular germ cell apoptosis: A functional proteomic study of experimental varicocele

Contact Info

Product

Resources

About