Seminal Glands of Buffalo Bulls (Bos bubalis L.) During Prepubertal and Pubertal Periods: Histological and Histochemical

Abstract

The current investigation was carried out on the seminal glands of six apparently healthy mature male Buffalo bulls and six immature. The specimens were collected and examined anatomically and histologically after being fixed in 10% buffered neutral formalin. The specimens were subjected to processing till paraffin sections were obtained and stained. The anatomical and histological findings of the seminal glands are varying with age. Anatomically, the immature seminal gland was small sized, flaccid in texture, white in color and measured about 0.80-0.95 gm in weight, 1.95-2.50 cm in length, 1.00-1.15 cm in width and about 0.46-0.60 cm in thickness. Meanwhile, in mature one became larger in size, firm in texture, pale yellow in color, multi-lobulated where had a very large lobules on its outer surface giving, cluster of grapes liked appearance. It measured about 7-8 gm in weight, 5-6 cm in length, 2-2.5 cm in width and about 80-1.3 cm in thickness. Microscopically, the seminal gland was observed consisting of a stroma of connective tissue and a parenchyma of differentiating and undifferentiating acini in immature animal. Meanwhile, in mature one, the parenchyma was consisted of secretory end pieces and well-developed duct system. With advancement of age, the thickness of the capsule and trabeculae increased, also the number of secretory end pieces per unite area increased and the amount of inter-glandular connective tissue stroma reduced.

Scrotal circumference: A predictor of testosterone concentration and certain attributes of seminal vesicles influencing buffalo male fertility

“…In the present study, varying tubular length with uni- or bi-lateral budding and lobulation patterns of shape could be due to increasing age, progressing SV weights, and testosterone levels as SV growth and function are androgen dependent [ 11 ]. Our findings corroborate with the findings in immature and mature buffalo bulls [ 17 ] and morphology during pre-pubertal, pubertal, and post-pubertal stage [ 18 ] in male buffalo. Furthermore, findings of SV histology are also comparable with reports in buffalo [ 17 ].…”

“…Our findings corroborate with the findings in immature and mature buffalo bulls [ 17 ] and morphology during pre-pubertal, pubertal, and post-pubertal stage [ 18 ] in male buffalo. Furthermore, findings of SV histology are also comparable with reports in buffalo [ 17 ].…”

“…Contrary to the present observation, a significant positive correlation was reported between SV weight and SV fluid testosterone in male buffalo [ 15 ]. The present study also recorded a significant effect of dentition age on SVs weight comparable to findings in water buffalo [ 26 ], while in another report, SV weight was lower in immature and mature buffalo bulls [ 17 ] possibly due to less number of observations and due to varying age and nutritional status which govern growth.…”

Melatonin administration induced reactivation in the seminal gland of the soay rams during non‐breeding season: An ultrastructural and morphometrical study

“…The macroscopic anatomy of the seminal vesicle varies among species where it is present. In rats and human, they are sac‐shaped (Brewster, ; Mukerjee and Rajan, ); in pigs, bulls and goats, they are compact and multilobulated (Badia et al, ; Samuelson, ; Ghonimi et al, ) and in ram, the seminal glands are compound tubular gland (Aitken, ). There are also species variations in factors that mediate and regulate the functions of these glands, such as androgen, prolactin and photoperiod (Lincoln and Ebling, ; Mukherjee and Haldar, 2014).…”

Characterization of vesicular glands, study of vesiculitis and its association with other accessory sex glands in bulls

“…The vesicular glands are the ones that contribute the most to semen (Alexander et al, 1971), producing substances that are responsible for the nutrition and survival of the sperm cell (Campos, 2003, Brackett, 2006Garner and Hafez, 2004), as well as decreasing its antigenicity and neutralizing the vaginal pH for fertilization to occur (Veselský, 1981;Gonzalez, 2002). The gland is an alveolar tubule type and has a relatively large lumen for storing secretions between ejaculates with pseudostratified columnar epithelium and sparse basal cells (Ghonimi et al, 2014a;Adhikary et al, 2015;Gofur, 2015).…”