Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance

Santiago-Moreno, J.; Bernal, B.; Pérez‐Cerezales, Serafín; Castaño, C.; Toledano-Dı́az, A.; Esteso, M.C.; Gutiérrez‐Adán, Alfonso; Gil, M. G.; Woelders, H.; Blesbois, Élisabeth

doi:10.1371/journal.pone.0209910

Cited by 46 publications

(55 citation statements)

References 61 publications

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“…The least abundant in our study, on the other hand, are tyrosine, methionine, alphaaminobutyric acid, allo-isoleucine, and asparagine, and are found to be similar with the low levels of amino acids of methionine and tyrosine in bull (Assumpção et al, 2005). Also, in domestic fowl, valine, serine, glycine, and alanine were the most abundant amino acids followed by glutamic acid (Santiago-Moreno et al, 2019). The alanine created a significant gene ontology in terms of its ligase activity and formed carbon-oxygen bonds, and is also involved in pathways of glycine, serine, alanine, and threonine metabolism.…”

Section: Discussionsupporting

confidence: 73%

“…In animal studies, supplementation of extender solutions with glutamine, glycine, and cysteine enhanced acrosome and membrane integrity of buffalo bull semen (El-Sheshtawy et al, 2008). Additionally, there was a positive correlation between membrane integrity and the concentration of valine, isoleucine and leucine, and lysine (Santiago-Moreno et al, 2019).…”

Section: Discussionmentioning

confidence: 90%

“…In other studies, researchers have determined different numbers of amino acids and peptides, in seminal plasma of bull, where glutamic and aspartic acid were the most abundant, and were associated with fertility and pregnancy rates (al-Hakim et al, 1970;Holden et al, 2017). Also, seminal plasma of human and other species were found to contain large numbers of amino acids (Engel et al, 2019;Santiago-Moreno et al, 2019). Fertility and sperm freezability are not always related.…”

Section: Discussionmentioning

confidence: 99%

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Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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Sperm cryopreservation is an important technique for fertility management, but postthaw viability of sperm differs among breeding bulls. With metabolites being the end products of various metabolic pathways, the contributions of seminal plasma metabolites to sperm cryopreservation are still unknown. These gaps in the knowledge base are concerning because they prevent advances in the fundamental science of cryobiology and improvement of bull fertility. The objective of this study was to test the hypothesis that seminal plasma amino acids are associated with freezability of bull sperm. To accomplish this objective, amino acid concentrations in seminal plasma from seven bulls of good freezability (GF) and six bulls of poor freezability (PF) were quantified using gas chromatography-mass spectrometry (GC-MS). Multivariate and univariate analyses were performed to identify potential freezability biomarkers. Pathways and networks analyses of identified amino acids were performed using bioinformatic tools. By analyzing and interpreting the results we demonstrated that glutamic acid was the most abundant amino acid in bull seminal plasma with average concentration of 3,366 ± 547.3 nM, which accounts for about 53% of total amino acids. The other most predominant amino acids were alanine, glycine, and aspartic acid with the mean concentrations of 1,053 ± 187.9, 429.8 ± 57.94, and 427 ± 101.3 nM. Pearson's correlation analysis suggested that phenylalanine concentration was significantly associated with post-thaw viability (r = 0.57, P-value = 0.043). Significant correlations were also found among other amino acids. In addition, partial least squares-discriminant analysis (PLS-DA) bi-plot indicated a distinct separation between GF and PF groups. Phenylalanine had the highest VIP score and was more abundant in the GF groups than in the PF groups. Moreover, pathway and network analysis indicated that phenylalanine contributes to oxidoreductase and antioxidant reactions. Although univariate analysis did not yield significant differences in amino acid concentration between the two groups, these findings are significant that they indicate the potentially important roles of amino acids in seminal plasma, thereby building a foundation for the fundamental science of cryobiology and reproductive biotechnology.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Ugur

Hitit

Kaya

et al. 2020

Front. Cell Dev. Biol.

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“…FAAs have been tested used for spermatozoa studies in different mammalian species, such as ram, goat, buffalo bull and stallion (Sanchez-Partida et al, 1992;Trimeche et al, 1999;Kundu and Das, 2001;Bucak et al, 2009;Tuncer et al, 2010;Topraggaleh et al, 2014), and also in poultry such as chicken (Santiago-Moreno et al, 2019). Although some contradictory results for spermatozoa of fish species, the positive and negative effects of FAAs at defined concentrations were reported more clearly for spermatozoa of mammalian species than of fish.…”

Section: Discussionmentioning

confidence: 99%

DNA damage, oxidative stress, decreased viability and motility in common carp (Cyprinuscarpio L.) spermatozoa induced by tryptophan, phenylalanine and cysteine amino acids during short-term storage

Kanyılmaz¹,

İnanan²

2020

Turk J Zool

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Free amino acids are found in spermatozoa and seminal plasma. Their qualitative and quantitative presence have been affected by many factors. Under in vitro storage conditions, their effects on spermatozoa in animal species and also human have been concerned over the last decades. In this study, the effects of tryptophan, phenylalanine and cysteine on DNA integrity, lipid peroxidation, viability and motility parameters in spermatozoa of the common carp (Cyprinuscarpio) were assessed during short-term storage. Spermatozoa were incubated at 1, 5, 25, and 50 mM of these amino acids in vitro at 4 °C in a time-dependent manner (6, 24, and 48 h). The supplementation of cysteine, phenylalanine and >5 mM of tryptophan to the incubation medium attenuated the spermatozoa motility parameters and viability. Particularly, >1 mM of cysteine completely inhibited progressive motility and decreased viability to <50%. Cysteine and phenylalanine at all concentrations significantly caused the increases in lipid peroxidation products in spermatozoa and seminal plasma, and hereby induced the percentage of DNA damage in spermatozoa. Cysteine had more detrimental effects than phenylalanine and tryptophan. Our data provide evidence that these amino acids are not beneficial for the maintaining of carp spermatozoa at 4 °C.

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“…Recent studies of sperm cryostability have established the effect of the amino acid profiles of seminal plasma in different breeds of chickens on DNA fragmentation (Santiago-Moreno et al, 2019). The composition of intracellular spermatozoon protein has been found to be associated with indicators of osmotic imbalance after thawing (Morris et al, 2012).…”

Section: Problems Of Cryopreservationmentioning

confidence: 99%

The current state of the problem of in vitrogene pool preservation in poultry

2020

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This review presents the current progress in and approaches to in vitroconservation of reproductive cells of animals, including birds, such as cryopreservation and freeze-drying, as well as epigenetic conditions for re storing viable spermatozoa and female gametes after conservation. Cryopreservation is an effective way to preserve reproductive cells of various species of animals and birds. In vitrogene pool conservation is aimed primarily to the restoration of extinct breeds and populations and to the support of genetic diversity in populations prone to genetic drift. It is the combination of ex situ in vivoand ex situ in vitromethods that can form the basic principles of the strategy of animal genetic diversity preservation. Also, use of cryopreserved semen allows faster breeding in industrial poultry farming. Despite numerous advances in semen cryobiology, new methods that can more efficiently restore semen fertility after cryopreservation are being sought. The mechanisms underlying the effect of cryopreservation on the semen parameters of cocks are insufficiently understood. The review reflects the results of recent research in the field of cryopreservation of female and male germ cells, embryonic cells, the search for new ways in the field of genetic diversity in vitro (the development of new cryoprotective media and new conservation technologies: freeze-drying). Molecular aspects of cryopreservation and the mechanisms of cryopreservation influence on the epigenetic state of cells are highlighted. Data on the results of studies in the field of male reproductive cell lyophilization are presented. The freeze-drying of reproductive cells, as a technology for cheaper access to the genetic material of wild and domestic animals, compared to cryopreservation, attracts the attention of scientists in Japan, Israel, Egypt, Spain, and France. There is growing interest in the use of lyophilized semen in genetic engineering technologies. Methods of freeze-drying are developed taking into account the species of birds. Organizational and legal ways of solving the problems of in vitroconservation of genetic resources of farm animals, including birds, are proposed.

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Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance

Cited by 46 publications

References 61 publications

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

Amino Acids of Seminal Plasma Associated With Freezability of Bull Sperm

DNA damage, oxidative stress, decreased viability and motility in common carp (Cyprinuscarpio L.) spermatozoa induced by tryptophan, phenylalanine and cysteine amino acids during short-term storage

The current state of the problem of in vitrogene pool preservation in poultry

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