Senescence‐associated mRNAs that may participate in signal transduction and protein trafficking

Guterman, Adi; Hajouj, Taleb; Gepstein, Shimon

doi:10.1034/j.1399-3054.2003.00116.x

Cited by 17 publications

(5 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present research also demonstrates senescence-induced expression of the genes encoding a calmodulin-regulated ion channel (At5g54250) and calciumbinding protein (At1g18210). Together with our recent observation concerning the involvement of a speci®c Ca 2 -dependent protein kinase in the regulation of leaf senescence (Guterman et al, 2003), these results suggest that Ca 2 may serve as a second messenger in the regulation of leaf senescence. Another interesting SAG represented by clone At1g68820 shows sequence homology to the animal apoptosis inhibitor (IAP) that regulates apoptosis by binding and inhibiting capsases (Lotocki and Keane, 2002).…”

Section: Regulatory Genessupporting

confidence: 78%

“…Recently, a few senescence-associated regulatory genes have been identi®ed. They are predicted to encode transcription factors (Eulgem et al, 2000;Hinderhofer and Zentgraf, 2001;Robatzek and Somssich, 2002), receptors for senescence perception (Hajouj et al, 2000;Robatzek and Somssich, 2002), and components of intracellular protein traf®cking (Guterman et al, 2003). Among the genes that are upregulated during leaf senescence are several whose transcript levels accumulate also under abiotic and biotic stresses (Binyamin et al, 2000;Hanfrey et al, 1996;John et al, 1997;Quirino et al, 1999;Weaver et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Large‐scale identification of leaf senescence‐associated genes

et al. 2003

Self Cite

View full text Add to dashboard Cite

SummaryLeaf senescence is a form of programmed cell death, and is believed to involve preferential expression of a speci®c set of`senescence-associated genes' (SAGs). To decipher the molecular mechanisms and the predicted complex network of regulatory pathways involved in the senescence program, we have carried out a large-scale gene identi®cation study in a reference plant, Arabidopsis thaliana. Using suppression subtractive hybridization, we isolated approximately 800 cDNA clones representing SAGs expressed in senescing leaves. Differential expression was con®rmed by Northern blot analysis for 130 non-redundant genes. Over 70 of the identi®ed genes have not previously been shown to participate in the senescence process. SAGencoded proteins are likely to participate in macromolecule degradation, detoxi®cation of oxidative metabolites, induction of defense mechanisms, and signaling and regulatory events. Temporal expression pro®les of selected genes displayed several distinct patterns, from expression at a very early stage, to the terminal phase of the senescence syndrome. Expression of some of the novel SAGs, in response to age, leaf detachment, darkness, and ethylene and cytokinin treatment was compared. The large repertoire of SAGs identi®ed here provides global insights about regulatory, biochemical and cellular events occurring during leaf senescence.

show abstract

Section: Regulatory Genessupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Large‐scale identification of leaf senescence‐associated genes

et al. 2003

Self Cite

View full text Add to dashboard Cite

show abstract

“…The other genes up-regulated in PI 312777 in response to barnyardgrass competition were those encoding kelch repeat-containing F-box family protein and RAD6 involved in protein degradation or repair pathway (Yamamoto et al 2004;Sun et al 2007); phosphatase 2C-like protein, PKD, GTP-binding protein, MYB, basic region leucine zipper and a putative senescence-associated protein functioning in signal transduction; and a Zn-finger, CONSTANS-type domain containing protein participating in gene regulation (Leung et al 1997;Sano and Ohashi 1995;Manning et al 2002;Guterman et al 2003;Landschulz et al 1988;Chen and Ni 2006). Therefore, the transcriptional activation of these genes in relative pathways can be an important step for PI 312777 in activating defense systems to adapt to the accompanying weed stress.…”

Section: Discussionmentioning

confidence: 99%

Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.)

Fang

Wang

et al. 2010

Plant Growth Regul

View full text Add to dashboard Cite

To explore the molecular mechanism of allelopathic rice in response to low nitrogen (N) supply or accompanying weed stress, allelopathic rice PI 312777 and its counterpart Lemont were grown under low N supply or co-cultured with barnyardgrass [Echinochloa crus-galli (L.) Beauv.] in hydroponics. The suppression subtractive hybridization (SSH) technique was employed to isolate the up-regulated genes in the treated rice accession. The results indicated that the expression of the genes associated with N utilization was significantly up-regulated in allelopathic rice PI 312777, and the higher efficiency of N uptake and its utilization were also detected in PI 312777 than that in Lemont when the two rice accessions were exposed to low N supply. This result suggested that the allelopathic rice had higher ability to adapt to low N stress than its nonallelopathic counterpart. However, a different response was observed when the allelopathic rice was exposed to accompanying weed (barnyardgrass) co-cultured in full Hoagland solution (normal N supply). It showed that the expression of the genes associated with allelochemical synthesis and its detoxification were all up-regulated in the allelopathic rice when co-cultured with the target weed under normal N supply. The results suggested that the allelopathic rice should be a better competitor in the riceweed co-culture system, which could be attributed to increasing de novo biosynthesis and detoxification of allelochemicals in rice, consequently resulting in enhanced allelopathic effect on the target and preventing the autotoxicity in this process. These findings suggested that the accompanying weed, barnyardgrass is not only the stressful factor, but also one of the triggers in activating allelopathy in rice. This implies that the allelopathic rice is sensible of the existing target in chemical communication.

show abstract

“…Molecular biological studies have shown a large range of genes that are expressed in senescing leaves and known as SAGs (senescenceassociated genes; Dangel et al 2000). Some of the SAGs are genes involving in regulatory processes such as phosphorylation and protein-protein interaction (Guterman et al 2003); one example is a gene encoding a putative senescence-related receptor kinase (Hajouj and Michelis 2000). Receptor kinases are involved in cellular signal transduction and also protein trafficking.…”

Section: Discussionmentioning

confidence: 99%

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Roshandel

Flowers

2008

Plant Soil

View full text Add to dashboard Cite

The effects of ionic stress on the physiology and gene expression of two rice genotypes (IR4630 and IR15324) that differ in salt tolerance, were investigated by evaluating changes in the biomass, Na + and K + concentrations and applying the cDNA-AFLP technique to highlight changes in gene expression. Over 8 days of salinisation, the effect of NaCl on the reduction of biomass (dry weight) was apparent from 24 h after salinisation (the first time point), indicating that the consequences of the build up of Na + (and Cl -) in the leaves of both lines was rapid. Furthermore, root growth of IR15324 was much more sensitive to salt than that of IR4630 (the reduction in root dry weight compared to nonsalinised plants was three times greater in IR15324 than IR4630). The two rice lines also differed in their Na + accumulation in saline conditions, a difference that was more marked in the shoots, particularly at the final harvest, than in the roots. Under salt stress, the K + content (µmol/shoot) increased over four successive harvests (24, 48, 96, 192 h) in both lines, but was always greater in IR4630 than in IR15324: differences in Na + /K + ratio appear to be an important determinant of salt tolerance in rice. To separate osmotic from ionic effects of salt, mannitol was applied as a non-ionic osmoticum at an osmotic potential estimated to be equivalent to 50 mM NaCl. Messenger RNA was sampled at 0.5, 6, 24, 48 and 192 hours after salinisation. Several products (AFLPbands) were detected, which were upregulated in the response to ionic effects of salt in the tolerant line (IR4630) and not expressed in the sensitive line (IR15324). Bioinformatic analysis indicated three of these AFLP-bands have a high-degree of sequence similarity with the genes encoding a proline rich protein, senescence associated protein and heat-shock protein. The data are novel in that they differentially highlight changes induced by the ionic rather than osmotic effects of salt and in a tolerant rather than a sensitive genotype. The possible roles of the products of these genes are discussed.

show abstract

Senescence‐associated mRNAs that may participate in signal transduction and protein trafficking

Cited by 17 publications

References 32 publications

Large‐scale identification of leaf senescence‐associated genes

Large‐scale identification of leaf senescence‐associated genes

Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.)

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Contact Info

Product

Resources

About