Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells

Wong, Pooi-Fong; Tong, Kind Leng; Jamal, Juliana; Khor, Eng Soon; Lai, Siew Li; Mustafa, Mohd Rais

doi:10.17179/excli2019-1505

Cited by 16 publications

(9 citation statements)

References 33 publications

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“…Membranes were blocked in PBS-bovine serum albumin (BSA) 5% for 1 h. Membranes were incubated with a rabbit anti-CD9 antibody (ab92726) (Abcam, Cambridge, UK) overnight at 4°C. This protein was one of those that showed the best signal in previous studies in HUVECs cells ( 19 , 20 ). Membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibody [VeriBlot for IP Detection Reagent (HRP) (ab131366)] for 2 h at room temperature.…”

Section: Methodsmentioning

confidence: 66%

EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells

et al. 2022

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BackgroundLittle is known about the effect of extra virgin olive (EVOO) and sunflower oil (SO) on the composition of extracellular vesicles (EVs) secreted by endothelial cells and the effects of these EVs on smooth muscle cells (SMCs). These cells play an important role in the development of atherosclerosis.MethodsWe evaluated the effects of endothelial cells-derived EVs incubated with triglyceride-rich lipoproteins obtained after a high-fat meal with EVOO (EVOO-EVs) and SO (SO-EVs), on the transcriptomic profile of SMCs.ResultsWe found 41 upregulated and 19 downregulated differentially expressed (DE)-miRNAs in EVOO-EVs. Afterwards, SMCs were incubated with EVOO-EVs and SO-EVs. SMCs incubated with SO-EVs showed a greater number of DE-mRNA involved in pathways related to cancer, focal adhesion, regulation of actin cytoskeleton, and MAPK, toll-like receptor, chemokine and Wnt signaling pathways than in SMCs incubated with EVOO-EVs. These DE-mRNAs were involved in biological processes related to the response to endogenous stimulus, cell motility, regulation of intracellular signal transduction and cell population proliferation.ConclusionEVOO and SO can differently modify the miRNA composition of HUVEC-derived EVs. These EVs can regulate the SMCs transcriptomic profile, with SO-EVs promoting a profile more closely linked to the development of atherosclerosis than EVOO-EVs.

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Section: Methodsmentioning

confidence: 66%

EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells

et al. 2022

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“…Exosome secretion is also particularly important in senescent ECs. Exposure of young ECs to exosomes from senescent ECs decreased the expression of inter-endothelial adherens junction proteins, VE-cadherin and beta-catenin ( Takasugi, 2018 ) leading to altered endothelial junctional communication including suppressed cell growth, proliferation and migration of ECs that disrupt the endothelial barrier integrity ( Wong et al, 2019 ).…”

Section: Endothelial Exosomesmentioning

confidence: 99%

Exosomal Composition, Biogenesis and Profiling Using Point-of-Care Diagnostics—Implications for Cardiovascular Disease

Burtenshaw

Regan

Owen

et al. 2022

Front. Cell Dev. Biol.

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Arteriosclerosis is an important age-dependent disease that encompasses atherosclerosis, in-stent restenosis (ISR), pulmonary hypertension, autologous bypass grafting and transplant arteriosclerosis. Endothelial dysfunction and the proliferation of vascular smooth muscle cell (vSMC)-like cells is a critical event in the pathology of arteriosclerotic disease leading to intimal-medial thickening (IMT), lipid retention and vessel remodelling. An important aspect in guiding clinical decision-making is the detection of biomarkers of subclinical arteriosclerosis and early cardiovascular risk. Crucially, relevant biomarkers need to be good indicators of injury which change in their circulating concentrations or structure, signalling functional disturbances. Extracellular vesicles (EVs) are nanosized membraneous vesicles secreted by cells that contain numerous bioactive molecules and act as a means of intercellular communication between different cell populations to maintain tissue homeostasis, gene regulation in recipient cells and the adaptive response to stress. This review will focus on the emerging field of EV research in cardiovascular disease (CVD) and discuss how key EV signatures in liquid biopsies may act as early pathological indicators of adaptive lesion formation and arteriosclerotic disease progression. EV profiling has the potential to provide important clinical information to complement current cardiovascular diagnostic platforms that indicate or predict myocardial injury. Finally, the development of fitting devices to enable rapid and/or high-throughput exosomal analysis that require adapted processing procedures will be evaluated.

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“…EVs containing C24:1 were taken up by primary BMSCs and eventually induced cellular senescence ( Khayrullin et al, 2019 ). Wong et al (2019) identified that EVs secreted from senescent HUVECs reduced the growth kinetics and barrier integrity of young HUVECs. These functions were realized by repressing the expression levels of adherens junction proteins, including vascular endothelial-cadherin and beta-catenin.…”

Section: The Effects Of Ev Components Derived From Senescent Cellsmentioning

confidence: 99%

“…These functions were realized by repressing the expression levels of adherens junction proteins, including vascular endothelial-cadherin and beta-catenin. Young HUVECs treated with senescence-derived EVs eventually showed reduced potential for impairing endothelial cell migration, indicating that the accumulation of senescent endothelial cells may have led to endothelial barrier dysfunction mediated by exposure to EVs ( Wong et al, 2019 ). Another study demonstrated a role for senescent fibroblast-derived EVs in regulating epidermal homeostasis.…”

Section: The Effects Of Ev Components Derived From Senescent Cellsmentioning

confidence: 99%

The Role of Extracellular Vesicles in Senescence

Koh

Jeon

et al. 2022

Molecules and Cells

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Cells can communicate in a variety of ways, such as by contacting each other or by secreting certain factors. Recently, extracellular vesicles (EVs) have been proposed to be mediators of cell communication. EVs are small vesicles with a lipid bilayer membrane that are secreted by cells and contain DNA, RNAs, lipids, and proteins. These EVs are secreted from various cell types and can migrate and be internalized by recipient cells that are the same or different than those that secrete them. EVs harboring various components are involved in regulating gene expression in recipient cells. These EVs may also play important roles in the senescence of cells and the accumulation of senescent cells in the body. Studies on the function of EVs in senescent cells and the mechanisms through which nonsenescent and senescent cells communicate through EVs are being actively conducted. Here, we summarize studies suggesting that EVs secreted from senescent cells can promote the senescence of other cells and that EVs secreted from nonsenescent cells can rejuvenate senescent cells. In addition, we discuss the functional components (proteins, RNAs, and other molecules) enclosed in EVs that enter recipient cells.

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Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells

Cited by 16 publications

References 33 publications

EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells

EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells

Exosomal Composition, Biogenesis and Profiling Using Point-of-Care Diagnostics—Implications for Cardiovascular Disease

The Role of Extracellular Vesicles in Senescence

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