Sennosides A and B Production by Hairy Roots of Senna alata (L.) Roxb.

Putalun, Waraporn; Pimmeuangkao, Suwat; De-Eknamkul, Wanchai; Tanaka, Hiroyuki; Shoyama, Yukihiro

doi:10.1515/znc-2006-5-612

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…(Putalun et al, 2006) and Datura quercifolia Kunth (Dupraz et al, 1994). Among the various sources of carbons, maltose at a concentration of 3% induces the highest percentage of hairy root cultures in leaves of Camellia sinensis (L.) Kuntze tea, followed by dextrose, sucrose, and fructose (John et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The sucrose concentration of 40 g L -1 was the best for fresh weight accumulation and hairy root growth of Angelica gigas Nakai (Xu et al, 2009), while 50 g L -1 sucrose mostly stimulated the growth of hairy roots of Senna alata (L.) Roxb. (Putalun et al, 2006) and Datura quercifolia Kunth (Dupraz et al, 1994). Among the various sources of carbons, maltose at a concentration of 3% induces the highest percentage of hairy root cultures in leaves of Camellia sinensis (L.) Kuntze tea, followed by dextrose, sucrose, and fructose (John et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Petrova¹,

Zayova²,

Dincheva³

et al. 2015

Turk J Biol

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Arnica montana L. (Asteraceae) is an economically important herb that contains numerous valuable biologically active compounds accumulated in various parts of the plant. The effects of carbon sources (sucrose, maltose, and glucose) at different concentrations (1%, 3%, 5%, 7%, and 9%) on growth were studied and GC-MS based metabolite profiling of A. montana hairy roots was conducted. The optimal growth and biomass accumulation of transformed roots were observed on an MS nutrient medium containing 3% or 5% sucrose. GC-MS analysis of hairy roots of A. montana showed the presence of 48 compounds in polar fractions and 22 compounds in apolar fractions belonging to different classes of metabolites: flavones, phenolic acids, organic acids, fatty acids, amino acids, sugars, sugar alcohols, hydrocarbons etc. Among the various metabolites identified, only the sugars and sugar alcohols were influenced by the concentration of the respective carbon sources in the nutrient medium.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Petrova¹,

Zayova²,

Dincheva³

et al. 2015

Turk J Biol

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“…Putalun et al (2006) reported that the growth rate of Senna alata hairy roots was increased in dark condition in a 1/2 MS medium containing 50 g L −1 sucrose. Growth and elicitation of hairy root cultures Investigation the effects of different concentrations of salicylic acid and chitosan on the growth rate of A. foeniculum hairy roots showed that application of salicylic acid reduced the growth of hairy roots compared with the control explants.…”

Section: Pcr Analysismentioning

confidence: 99%

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

2014

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Hairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, onemonth-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L −1 ) (as elicitor) and sucrose (20, 30, 40 and 50 g L −1 ) on the growth of hairy roots were evaluated. The results showed that, 30 g L −1 sucrose and 100 mg L −1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

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Secondary Metabolites of Medicinal Plants

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Sennosides A and B Production by Hairy Roots of Senna alata (L.) Roxb.

Cited by 8 publications

References 21 publications

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

Influence of carbon sources on growth and GC-MS basedmetabolite profiling of Arnica montana L. hairy roots

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

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