Sensing of epigallocatechin gallate and tannic acid based on near infrared optical spectroscopy of DNA-wrapped single-walled carbon nanotube hybrids

Abstract

The optical responses of DNA-wrapped single-walled carbon nanotubes (DNA-SWNTs) caused by the addition of epigallocatechin gallate (EG) and tannic acid (TA) were investigated. Near infrared absorbance and photoluminescence spectra of pre-oxidized DNA-SWNTs were measured with and without EG and TA. Since both EG and TA have antioxidant abilities, the near infrared spectra changed after the addition of the chemicals. While detection of the antioxidant abilities of EG by DNA-SWNT hybrids has been previously reported, TA was employed for the first time. The changes in the absorbance caused by the addition of TA were found to saturate at 0.33 µM TA, although saturation was not observed even at 3.3 µM with the addition of EG. Oxidation of DNA-SWNT was suppressed by addition of TA or EG. The effects of TA were much greater than those of EG on the absorbance. The photoluminescence did not appear to saturate at 3.3 µM for either EG or TA. photoluminescence had a larger measurement capacity than NIR absorbance. Interestingly, the photoluminescence change caused by EG was much larger than that of TA at 3.3 µM, although the antioxidant property of TA was stronger at 0.33 and 0.033 µM, suggesting that the optical responses were not simple redox reactions. Peak shifts in the absorbance/photoluminescence spectra revealed unique differences according to the chirality of the SWNTs and the types of chemicals used. This suggests that the combination of absorbance/photoluminescence and peak intensity/peak shifts could detect multiple types of chemical information, not only redox reaction. A direct comparison between EG and TA was demonstrated for the first time in this novel study. In addition, sensing of EG and TA by near infrared spectra was made available using not only changes in intensity but also a variety of peak shifts of SWNTs.

Detection and Imaging of the Plant Pathogen Response by Near‐Infrared Fluorescent Polyphenol Sensors

“…In addition to the change in emission of PEG-PL-SWCNTs also E 11 absorption maximum was redshifted by % 10 nm (Supplementary Figure S2). Overall, such interplay indicates as ensing mechanism based on ac hange in fluorescence quantum yield, without dominant aggregation effects.I t furthermore suggests as trong interaction between sensor and analyte that goes beyond polyphenols acting as antioxidants [60,61] and might include changes in solvation that affects exciton diffusion. Hence,PEG-PL-SWCNTs showed the most promising response to plant polyphenols.…”

Detektion und Visualisierung der Pflanzen‐Pathogen‐Response durch Nah‐Infrarot‐fluoreszente Polyphenolsensoren

“…Insgesamt deutet ein solches Zusammenspiel auf einen Sensormechanismus hin, der auf einer ¾nderung der Fluoreszenzquantenausbeute ohne dominante Aggregationseffekte beruht. Darüber hinaus deutet dies auf eine starke Wechselwirkung zwischen Sensor und Analyt hin, die über die als Antioxidantien wirkenden Polyphenole hinausgeht [60,61] und Veränderungen der Solvatation beinhalten kçnnte,w elche die Exzitonendiffusion beeinflusst. Zusammenfassend zeigten daher PEG-PL-SWCNTs die vielversprechendste Reaktion auf Pflanzenpolyphenole.…”

Distinguishing Antioxidant Molecules with Near-Infrared Photoluminescence of DNA-Wrapped Single-Walled Carbon Nanotubes

“…Hamano et al studied the antioxidant ability of catechin using NIR absorbance (NIR-ABS) values of DNA-SWNTs, 33 whereas Matsukawa and Umemura analyzed it by means of NIR photoluminescence (NIR-PL) intensities of DNA-SWNTs, 34 and Yamazaki and Umemura analyzed the antioxidant potencies of epigallocatechin gallate and tannic acid using NIR-PL and NIR-ABS, and the NIR-PL results showed higher antioxidant capacities. 35 NIR-PL spectroscopy was employed to assess the optical responses of DNA-SWNTs to an oxidant (KMnO 4 ) and antioxidant biomolecules (saponin and banana biomolecules). The differences in the PL responses of SWNTs, depending on their chemical interactions with different molecules, such as DNA and biomolecules, were successfully investigated.…”

“…The antioxidant potencies of saponin and banana solutions have been analyzed using traditional methods by various research groups. ,,− However, the utilization of the optical responses of DNA-wrapped SWNTs (DNA-SWNTs) in the near-infrared (NIR) region has promised a modern technique for analyzing and distinguishing antioxidant biomolecules. Hamano et al studied the antioxidant ability of catechin using NIR absorbance (NIR-ABS) values of DNA-SWNTs, whereas Matsukawa and Umemura analyzed it by means of NIR photoluminescence (NIR-PL) intensities of DNA-SWNTs, and Yamazaki and Umemura analyzed the antioxidant potencies of epigallocatechin gallate and tannic acid using NIR-PL and NIR-ABS, and the NIR-PL results showed higher antioxidant capacities . NIR-PL spectroscopy was employed to assess the optical responses of DNA-SWNTs to an oxidant (KMnO 4 ) and antioxidant biomolecules (saponin and banana biomolecules).…”