Sensitive and rapid high-performance liquid chromatographic method for simultaneous determination of antidepressants in pharmaceutical formulations

Abstract

SummaryAn easy, rapid, and sensitive high-performance liquid chromatographic (HPLC) method is proposed for simultaneous determination of five antidepressants (trazodone, citalopram, fluoxetine, fluvoxamine and clomipramine) and norfluoxetine, a major metabohte of fluoxetine. Optimum conditions for the quantitative separation were investigated. The compounds were separated on a Nova Pack Cls column with acetonitrile-pH 2.5 phosphate buffer, 40:60 (v/v), as mobile phase at a flow rate of 1.5 mL min 1.. Stability of solutions, hnearity, repeatability and reproducibility, accuracy, specificity, and detection and quantification limits were examined to validate the method. The method is rapid and sensitive and can be applied to the analysis of antidepressants in pharmaceutical formulations and biological fluids; norfluoxetine can also be determined in these matrices. We have tested the method by analysis of several pharmaceutical formulations; recoveries obtained were bef,,veen 102.7% and 98.2% of the labelled values.

Sensitive capillary GC‐MS‐SIM determination of selective serotonin reuptake inhibitors: Reliability evaluation by validation and robustness study

“…Several HPLC methods have been reported for the determination of FLX, CIT, PAX and their metabolites in pharmaceutical formulations [9], and in plasma or serum [10,11]; in addition, the determination of SSRIs in whole blood using UV and fluorescence detection has been published [12].…”

Electroanalytical study of fluvoxamine

“…Most FVX analysis is performed by high-perfor-mance liquid chromatography (HPLC) coupled with spectrometric detectors, ultraviolet/diode array [4][5][6][7][8][9][10][11][12][13][14][15][16][17], mass spectrometric [18,19] and fluorimetric [20][21][22], for analysis of pharmaceuticals [8] and human serum or plasma [4][5][6][7][10][11][12][13][14][15][16][17][18][19][20][21][22]. This widespread usage of HPLC methods is justified by the high sensitivity and the low limit of detection usually obtained and the possibility of simultaneous analysis of the drug and its metabolites, which increases its use even further.…”

Quality assessment of fluoxetine and fluvoxamine pharmaceutical formulations purchased in different countries or via the Internet by 19F and 2D DOSY 1H NMR

“…Two of them were the brand formulations from LILLY (Prozac ® ) and SOLVAY Pharma (Floxyfral ® ), the others were generic drugs from different countries. The declared amounts of the active ingredient in the various Table 1 Commercial formulations of fluoxetin (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) and fluvoxamine (16)(17)(18)(19) All samples, as received, were stored in the dark at ambient temperature and humidity. They were all analysed within expiry dates.…”

“…1 H NMR experiments were performed on a Bruker AVANCE 500 spectrometer operating at 500.13 MHz equipped with a 5 mm proton cryoprobe at 298 K on 600 L samples.…”