Sensitive cell-based assay using DCFH oxidation for the determination of pro- and antioxidant properties of compounds and mixtures: Analysis of fruit and vegetable juices

Girard-Lalancette, Karl; Pichette, André; Legault, Jean

doi:10.1016/j.foodchem.2008.12.002

Cited by 118 publications

(90 citation statements)

References 28 publications

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“…Reactive oxygen species (ROS), including oxygen radicals and their reactive products, are known to react with biological molecules such as proteins, lipids and DNA, leading to cellular and tissue damage (Girard-Lalancette et al, 2009). It is well known that ROS can induce both apoptosis and autophagy.…”

Section: Discussionmentioning

confidence: 99%

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Banjerdpongchai¹,

Khaw-on²

2013

Asian Pacific Journal of Cancer Prevention

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Section: Discussionmentioning

confidence: 99%

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Banjerdpongchai¹,

Khaw-on²

2013

Asian Pacific Journal of Cancer Prevention

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“…Antioxidant activity was evaluated using the DCFH-DA assay as described by Girard-Lalancette et al, 18 with some modifications. In brief, WS1 cells were plated in 96-microwell plates at 10,000 cells per well and incubated for 24 hours at 378C and 5% CO 2 .…”

Section: Antioxidant Cell-based Assaymentioning

confidence: 99%

Antioxidant Activity, Inhibition of Nitric Oxide Overproduction, andIn VitroAntiproliferative Effect of Maple Sap and Syrup fromAcer saccharum

Legault

Girard-Lalancette

Grenon

et al. 2010

Journal of Medicinal Food

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Antioxidant activity, inhibition of nitric oxide (NO) overproduction, and antiproliferative effect of ethyl acetate extracts of maple sap and syrup from 30 producers were evaluated in regard to the period of harvest in three different regions of Québec, Canada. Oxygen radical absorbance capacity (ORAC) values of maple sap and syrup extracts are, respectively, 12 +/- 6 and 15 +/- 5 micromol of Trolox equivalents (TE)/mg. The antioxidant activity was also confirmed by a cell-based assay. The period of harvest has no statistically significant incidence on the antioxidant activity of both extracts. The antioxidant activity of pure maple syrup was also determined using the ORAC assay. Results indicate that the ORAC value of pure maple syrup (8 +/- 2 micromol of TE/mL) is lower than the ORAC value of blueberry juice (24 +/- 1 micromol of TE/mL) but comparable to the ORAC values of strawberry (10.7 +/- 0.4 micromol of TE/mL) and orange (10.8 +/- 0.5 micromol of TE/mL) juices. Maple sap and syrup extracts showed to significantly inhibit lipopolysaccharide-induced NO overproduction in RAW264.7 murine macrophages. Maple syrup extract was significantly more active than maple sap extract, suggesting that the transformation of maple sap into syrup increases NO inhibition activity. The highest NO inhibition induced by the maple syrup extracts was observed at the end of the season. Moreover, darker maple syrup was found to be more active than clear maple syrup, suggesting that some colored oxidized compounds could be responsible in part for the activity. Finally, maple syrup extracts (50% inhibitory concentration = 42 +/- 6 microg/mL) and pure maple syrup possess a selective in vitro antiproliferative activity against cancer cells.

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“…In a normal healthy cell, equilibrium between the generation of ROS and their elimination by the antioxidant system is maintained. ROS is a key regulator of homeostasis, whereas the increased generation of ROS plays an important role in inflammation, host defense response and tissue repair (Girard-Lalancette et al, 2009). The molecules including superoxide, hydroxyl radicals, nitric oxide (NO), and hydrogen peroxide commonly have been designated as ROS (McArdle et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory and antioxidant activity of a Siraitia grosvenorii polysaccharide in mice

Zhang¹,

Hu²,

Lu³

2011

Afr. J. Biotechnol.

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The aim of this present study was to investigate the immunomodulatory and antioxidant activities of Siraitia grosvenorii polysaccharide (SGP) using a mouse model. The roles of regulation of SGP were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, fluorescence method and ELISA method. The chemical structures were analyzed by Fourier transform infrared spectroscopy (FTIR) and gas chromatography (GC). The results show that SGP promoted the proliferation of spleen cells and regulated the level of reactive oxygen species (ROS) in vitro. Furthermore, it significantly raised spleen and thymus indices and superoxidase dismutase activity and regulated the cytokine levels of spleen and thymus in mice in vivo. These findings suggest that SGP possesses immunomodulatory and antioxidant effects.

show abstract

Sensitive cell-based assay using DCFH oxidation for the determination of pro- and antioxidant properties of compounds and mixtures: Analysis of fruit and vegetable juices

Cited by 118 publications

References 28 publications

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Antioxidant Activity, Inhibition of Nitric Oxide Overproduction, andIn VitroAntiproliferative Effect of Maple Sap and Syrup fromAcer saccharum

Immunomodulatory and antioxidant activity of a Siraitia grosvenorii polysaccharide in mice

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