Sensitive detection of a serum biomarker based on peptide nucleic acid-coupled dual cycling reactions

Zhang, Yuanyuan; Li, Hao; Huang, Yue; Yin, Tingting; Sun, Lizhou; Li, Genxi

doi:10.1016/j.aca.2015.05.006

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Zhang et al have developed a highly sensitive and selective method for protein or hormone markers assay [66]. This method takes advantage of PNA-coupled dual amplification simultaneously both in solution and on electrode interface, as well as Zr 4+ mediated phosphate group's interaction, receiving greatly increased sensitivity of the detection and efficiently monitor the insulin level for the evaluation of serum insulin in pregnant women.…”

Section: Single Nucleotide Polymorphisms (Snps) Detection Using Pnamentioning

confidence: 99%

A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

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Peptide Nucleic Acid (PNA) is a nucleobase oligomer in which the whole backbone is mainly replaced by N-(2aminoethyl) glycine units. PNA is considered as DNA with a neutral peptide backbone due to negative charged sugar-phosphate backbone. It is chemically stable and resistant to hydrolytic cleavage. PNA can be categorized specific sequences of DNA and RNA according to Watson-Crick hydrogen bonding structure. Hybridization process showed high thermal stability and unique ionic strength effects. It is formed a stable PNA/DNA/PNA triplex with a looped-out DNA strand. PNA hybridization technology is promptly developed within in situ hybridization. In our review paper was elaborated the PNA superior hybridization characteristics, importance's of PNA and major applications of PNA in the diagnostic and pharmaceutical fields. And also PNA could be replaced DNA in uses as a probe for many investigation purposes. PNAs antisense activities have found in nerve cells and even in rats upon injection into the brain, and in Escherichia coli.

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Section: Single Nucleotide Polymorphisms (Snps) Detection Using Pnamentioning

confidence: 99%

A Review of Peptide Nucleic Acid

Siddiquee¹,

Rovina²,

Azriah³

2015

Adv Tech Biol Med

View full text Add to dashboard Cite

show abstract

“…The polymerase chain reaction (PCR) can amplify a specific DNA strand by a factor of 10 6 and be widely considered as the gold standard, however, constrained by thermal cycling . To simplify operation, isothermal amplification methods are developed, such as loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), and recombinase polymerase amplification (RPA). , The above methods adopt various nucleases (polymerases, endonucleases, and exonucleases) that are costly and expressed by microbes and show the turnover number ( k cat ) less than hundreds of nucleotides per second. − Alternatively, DNAzyme and nucleic acid strand displacement reactions can also achieve signal amplification without using any enzymes. − Despite the great progress, novel amplification strategies are highly desired for biosensors.…”

Section: Introductionmentioning

confidence: 99%

Dual Signal Amplification by Urease Catalysis and Silver Nanoparticles for Ultrasensitive Colorimetric Detection of Nucleic Acids

et al. 2023

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Signal amplification techniques are highly desirable for the analysis of low-level targets that are closely related with diseases and the monitoring of important biological processes. However, it is still challenging to achieve this goal in a facile and economical way. Herein, we developed a novel dual signal amplification strategy by combining urease catalysis with the release of Ag+ from silver nanoparticles (AgNPs). This strategy was used for quantifying a DNA sequence (HIV-1) related with human immunodeficiency virus (HIV). DNA target HIV-1 hybridizes with the capture DNA probe on magnetic beads and the reporter DNA probe on AgNPs, forming a sandwich complex. The captured AgNPs are then transformed into numerous Ag+ ions that inactivate numerous ureases. Without catalytic production of ammonia from urea, the substrate solution shows a low pH 5.8 that will increase otherwise. The pH change is monitored by a pH indicator (phenol red), which allows for colorimetric detection. The proposed approach is sensitive, easy to use, economic, and universal, exhibiting a low detection limit of 9.7 fM (i.e., 1.94 attomoles) and a dynamic linear range of 4 orders for HIV-1 sequence detection.

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Electrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy

et al. 2016

Analytica Chimica Acta

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Sensitive detection of a serum biomarker based on peptide nucleic acid-coupled dual cycling reactions

Cited by 3 publications

References 25 publications

A Review of Peptide Nucleic Acid

A Review of Peptide Nucleic Acid

Dual Signal Amplification by Urease Catalysis and Silver Nanoparticles for Ultrasensitive Colorimetric Detection of Nucleic Acids

Electrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy

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