Sensitive detection of clenbuterol by hybrid iridium/silicon nanowire-enhanced laser desorption/ionization mass spectrometry

Lv, Rui; Wu, Enhui; Wu, Renfei; Shen, Wen; Ma, Chongqing; Shi, Ruifeng; Guo, Ruochen; Shao, Mingwang; Liu, Jian

doi:10.1039/d0tb00186d

Cited by 9 publications

(8 citation statements)

References 54 publications

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“…1 In addition, CL has an ability to improve the decomposition and transformation of fat and is used as a kind of additive in feed to speed growth and produce more muscle of animals. 2,3 However, CL is relatively stable, which makes it difficult to decompose in animal tissues and easy to accumulate. Excess ingestion of this drug in animals can lead to acute poisoning, muscle tremors, heart palpitations, and even death in severe cases.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Clenbuterol (CL), an artificially synthesized β 2 -adrenergic agonist, has been widely used to treat bronchial asthma and lung disease . In addition, CL has an ability to improve the decomposition and transformation of fat and is used as a kind of additive in feed to speed growth and produce more muscle of animals. , However, CL is relatively stable, which makes it difficult to decompose in animal tissues and easy to accumulate. Excess ingestion of this drug in animals can lead to acute poisoning, muscle tremors, heart palpitations, and even death in severe cases. , As a result, many countries have prohibited its use in food production .…”

Section: Introductionmentioning

confidence: 99%

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Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Tian

et al. 2021

Anal. Chem.

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Lateral flow immunoassay (LFIA) has emerged as an effective technique in the field of food safety and environmental monitoring. However, sensitive and quantitative detection is still challenging for LFIAs in complex environments. In this work, a dual-model colorimetric/SERS lateral flow immunoassay for ultrasensitive determination of clenbuterol was constructed based on a metallic core–shell Au/Au nanostar acting as a multifunction tag. Raman reporter molecules are located between the core (AuNP) and shell (Au nanostar) to form a sandwich structure, which contributes to eliminate the environmental interference and improve the detection stability. In addition, the Au/Au nanostar provides a much higher Raman enhancement due to the presence of sharp tips and larger surface roughness in comparison with gold nanoparticles (AuNPs). Thus, on the basis of the antibody–antigen interaction, the dual-model immunoassay can produce strong colorimetric and surface-enhanced Raman spectroscopy (SERS) signals for highly sensitive detection of the target analyte, clenbuterol. Under optimal conditions, clenbuterol could be detected by the colorimetric model with a visual detection limit of 5 ng/mL. Meanwhile, the SERS signal of the Au/Au nanostar was accumulated on the test line for the SERS model detection with a quantitative detection limit as low as 0.05 ng/mL, which is at least 200-fold lower than that of the traditional AuNPs-based immunoassay. Furthermore, recovery rates of the proposed method in food samples were 86–110%. This dual-model immunoassay provides an effective tool for antibiotic residues analysis and demonstrates a broad potential for future applications in food safety monitoring.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Tian

et al. 2021

Anal. Chem.

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“…3 FM: fluorescent microsphere. 4 TRFN: time-resolved fluorescent nanobead. 5 UCNPs: up-conversion NPs.…”

Section: Novel Labels For Use In Lfas To Detect β-Adrenergic Agonist ...mentioning

confidence: 99%

“…The synthesized β-adrenergic agonist family (“lean meat powder”) includes clenbuterol (CLE), ractopamine (RAC), salbutamol (SAL), terbutaline, and clorprenaline, and they have been widely used to treat lung disease and asthma in humans. Furthermore, members of this family can also be used to improve fat decomposition and conversion and are therefore used as feed additives to accelerate growth and increase muscle in animals [ 3 , 4 ]. However, long-term or high-dose intake of residual β-adrenergic agonists through meat products can cause serious and harmful physiological side effects such as myalgia, dizziness, tachycardia, nervousness, and even death [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

et al. 2022

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The illegal use of β-adrenergic agonists during livestock growth poses a threat to public health; the long-term intake of this medication can cause serious physiological side effects and even death. Therefore, rapid detection methods for β-adrenergic agonist residues on-site are required. Traditional detection methods such as liquid chromatography have limitations in terms of expensive instruments and complex operations. In contrast, paper methods are low cost, ubiquitous, and portable, which has led to them becoming the preferred detection method in recent years. Various paper-based fluidic devices have been developed to detect β-adrenergic agonist residues, including lateral flow immunoassays (LFAs) and microfluidic paper-based analytical devices (μPADs). In this review, the application of LFAs for the detection of β-agonists is summarized comprehensively, focusing on the latest advances in novel labeling and detection strategies. The use of μPADs as an analytical platform has attracted interest over the past decade due to their unique advantages and application for detecting β-adrenergic agonists, which are introduced here. Vertical flow immunoassays are also discussed for their shorter assay time and stronger multiplexing capabilities compared with LFAs. Furthermore, the development direction and prospects for the commercialization of paper-based devices are considered, shedding light on the development of point-of-care testing devices for β-adrenergic agonist residue detection.

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“…1,2 Unfortunately, the compound is also abused as an additive in animal husbandry to improve the animal growth rate and lean meat rate. 3,4 Due to its long half-life and slow metabolic rate, excessive CLB will accumulate in animals and enter the human body through meat products, and people who eat contaminated animal products may experience tachycardia, nausea, dizziness, headaches, fever, vertigo and chills. 3,5,6 Therefore, many countries prohibit the addition of clenbuterol to animal feed.…”

Section: Introductionmentioning

confidence: 99%

A label-free aptasensor for rapid detection of clenbuterol based on SYBR Green I

Xiao

Sun

et al. 2022

New J. Chem.

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Sensitive detection of clenbuterol by hybrid iridium/silicon nanowire-enhanced laser desorption/ionization mass spectrometry

Abstract: There is an increasing demand in anti-doping drug monitoring in sports and food safety check by developing sensitive and fast analytical methods. Here we report the development of hybrid Ir/SiNW...

Cited by 9 publications

References 54 publications

Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

A label-free aptasensor for rapid detection of clenbuterol based on SYBR Green I

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