Sensitive Detection of <i>Aeromonas Hydrophila</i> by Time-Resolved Fluorimmunoassay

Lin, Peng; Lu, Jie; Guo, Songlin; Feng, Juan; Wang, Yilei; Chen, Jinmin

doi:10.6023/a16110619

Cited by 3 publications

(1 citation statement)

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“…In the intensive high-density aquaculture, cultivated eels are vulnerable to infection by bacterial pathogens, and studies have found that economic losses caused by bacterial diseases account for about 90% (Guo, 2006(Guo, , 2011. Our studies in the recent 18 years found that Vibrio vulnificus, Edwardsiella anguillarum and Aeromonas hydrophila are the most common bacterial pathogens in eel culture (Duan et al, 2019;Guo, Yang, Feng, Duan, & Zhao, 2016;Lin et al, 2017). These three pathogens cause various diseases during the alternation of spring and summer, and infectious diseases such as liver ulcer, kidney enlargement, septicaemia and rotten gill and tail occur in Japanese eel (Anguilla japonica), American eel (Anguilla rostrata) and European eel (Anguilla anguilla; Amaro et al, 2015;Buján et al, 2018;Guo, 2006;Guo, 2006;Reichley et al, 2017).…”

Section: Introductionmentioning

confidence: 93%

Construction expression and immunogenicity of a novel trivalent outer membrane protein (OmpU‐A‐II) from three bacterial pathogens in Japanese eels (Anguilla japonica)

Zhao

Zhai

et al. 2020

Journal of Fish Diseases

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Vibrio vulnificus, Edwardsiella anguillarum and Aeromonas hydrophila are three common bacterial pathogens in cultivated eels. To protect farming eels from infection by these pathogens, a trivalent outer membrane protein (OMP) containing partial sequences of OmpU from V. vulnificus, OmpA from E. anguillarum and OmpII from A. hydrophila was expressed and purified; then, the OMP was used as a vaccine to immunize Japanese eels (Anguilla japonica). Whole-blood cell proliferation, antibody titres and complement and lysozyme activities were detected at different days postimmunization (dpi), and the relative per cent survival (RPS) was determined after eels were infected with V. vulnificus, E. anguillarum or A. hydrophila at 28 dpi. The results showed that the OMP significantly stimulates the antibody titres. At 14 days after the challenge (i.e. at 28 dpi), the RPS of OMP against V. vulnificus, E. anguillarum and A. hydrophila was 20%, 70% and 11.1%, respectively. The construction, expression and immunogenicity of a trivalent Omp were reported for the first time, and this study will provide a valuable reference for the development of fish multiplex vaccines. K E Y W O R D SAeromonas hydrophila, Edwardsiella anguillarum, immunogenicity, trivalent Omp, Vibrio vulnificus How to cite this article: Zhao J, Wu L, Zhai S, Lin P, Guo S.Construction, expression and immunogenicity of a novel trivalent outer membrane protein (OmpU-A-II) from three bacterial pathogens in Japanese eels (Anguilla japonica). J Fish

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