Sensitive Detection of Near-Infrared Fluorescent Dyes Using High-Performance Liquid Chromatography with Peroxyoxalate Chemiluminescence Detection System

Near-infrared chemiluminescence tunable from 900 nm to 1700 nm from narrow-bandgap compounds and polymers

“…23,25,26,31 It is known that NIR CL can be generated from gas-phase reactions, 32 oxidation of ammonia, 33 singlet oxygen 34,35 and NIR fluorescent dyes by energy transfer in the peroxyoxalate reaction. 36,37 However, due to the lack of highly efficient NIR fluorescent dyes, CL generated from the peroxyoxalate-dye system still lies in the spectral region outside of the desirable biological (900-1200 nm) and telecommunication (1300-1600 nm) windows. 25,26,36,38 Herein we report, for the first time, NIR CL tunable from 900 nm to 1700 nm using narrow-bandgap compounds and polymers.…”

Analysis of methylene blue in human urine by capillary electrophoresis

“…The extraction of MB from urine was improved by adding sodium hexanesulfonate as ion-paring reagent [2][3][4] compared to sodium chloride and other inorganic salts [2]. Most analytical assays used HPLC with a wide range of columns including lipophilic RP18 columns [6,[11][12][13][14][15], polar cyano columns [5,8], and an ion chromatography column [16] coupled to different optical [5,6,8] or mass spectroscopic detectors [14,15]. In spite of the superior separation efficiency of capillary electrophoresis (CE) and the ionic structure of MB only sporadic information of the electrophoretic behaviour of MB has been reported [17][18][19][20][21][22].…”

Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye

“…Some NIR dyes such as pentamenthine cyanine, anthraquinonoid, phthalocyanine, and nickel complex dye have been reported as functional dyes for optical recording media for semiconductor lasers [10]. Although the NIR dyes have been gradually used in spectrofluorimetric analysis [11,12,13], capillary electrophoresis (CE) [14,15,16,17,18,19,20] and high-performance liquid chromatography (HPLC) [21,22] with laserinduced fluorescence (LIF) detection, very few applications to spectrophotometric quantitation of proteins with NIR dyes have been reported.…”