Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye

Li, Wei-Rong; Wang, Hong; Yang, Tie; Zhang, Hua‐Shan

doi:10.1007/s00216-003-2100-1

Analytical and Bioanalytical Chemistry

2003

DOI: 10.1007/s00216-003-2100-1

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Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye

Wei-Rong Li

Hong Wang

Tie Yang

et al.

Abstract: A new near-infrared (NIR) dye, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DTCY) has been developed for the quantitation of proteins in solution. The method is based on the binding of DTCY to proteins under acidic conditions. The binding of DTCY to proteins causes a new band at 814 nm. The maximum binding number of bovine serum albumin (BSA) with DTCY was measured as 100. The linear range is 0.3-40 microg mL(-1) for BSA and human serum albumin (HSA), respectively. Except for Fe(2+), Cu(2+), and… Show more

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Cited by 11 publications

References 26 publications

(23 reference statements)

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Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine

Wang

Guo

et al. 2007

Anal Bioanal Chem

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The application of near-infrared (NIR) dyes (lambda (em) > 750 nm) to the analysis of biological samples shows much promise, because the long emission wavelengths of such dyes allow interferences from biomolecule matrices to be minimized. In this paper, a novel NIR dye, 5,5'-dicarboxy-1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DCDSTCY) has been developed for the spectrophotometric determination of total protein in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportional to the concentration of protein. The linear range of the method was found to be 0.04-0.5 microg mL(-1) for bovine serum albumin (BSA) and human serum albumin (HSA), and detection limits of 5 ng mL(-1) were obtained for these substances. The maximum binding number of BSA with DCDSTCY was measured to be 133. The method proposed here has been applied to the quantitation of total protein in serum, and recoveries of 96.6-104% were achieved. Figure Near-infrared probe for protein determination.

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Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine

Wang

Guo

et al. 2007

Anal Bioanal Chem

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Study of the feasibility of distinguishing cigarettes of different brands using an Adaboost algorithm and near-infrared spectroscopy

Tan

Xin

2007

Anal Bioanal Chem

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The feasibility of utilizing an Adaboost algorithm in conjuction with near-infrared (NIR) spectroscopy to automatically distinguish cigarettes of different brands was explored. Simple linear discriminant analysis (LDA) was used as the base algorithm to train all weak classifiers in Adaboost. Both principal component analysis (PCA) and its kernel version (kernel principal component analysis, KPCA) were used for feature extraction and were also compared to each other. The influence of the training set size on the final classification model was also investigated. Using a case study, it was demonstrated that Adaboost coupled with PCA or KPCA can obviously improve the ability to discriminate between samples that cannot be separated by a single linear classifier. However, in term of the overall performance, KPCA appears preferable to PCA for feature extraction, especially when the samples used for training are relatively small. The results also indicate that more training samples should be applied, if possible, in order to fully demonstrate the superiority of Adaboost. It seems that the use of an Adaboost algorithm in conjunction with NIR spectroscopy in combination with KPCA for feature extraction comprises a promising tool for distinguishing cigarettes of different brands, especially in situations where there is an obvious overlap between the NIR spectra afforded by cigarettes of different brands.

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Determination of total protein in hyperimmune serum samples by near-infrared spectrometry and multivariate calibration

Baddini

Cunha

Oliveira

et al. 2010

Analytical Biochemistry

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Direct spectrometric determination of proteins in body fluids using a near-infrared cyanine dye

Cited by 11 publications

References 26 publications

Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine

Spectrophotometric determination of total protein in serum using a novel near-infrared cyanine dye, 5,5′-dicarboxy-1,1′-disulfobutyl-3,3,3′,3′-tetramethylindotricarbocyanine

Study of the feasibility of distinguishing cigarettes of different brands using an Adaboost algorithm and near-infrared spectroscopy

Determination of total protein in hyperimmune serum samples by near-infrared spectrometry and multivariate calibration

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