Determination of total protein in hyperimmune serum samples by near-infrared spectrometry and multivariate calibration

Baddini, Ana Luiza Q.; Cunha, Luiz Eduardo Ribeiro da; Oliveira, Antonia Maria Cavalcanti de; Cassella, Ricardo J.

doi:10.1016/j.ab.2009.10.012

Cited by 13 publications

(8 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our current study, we chose to observe the morphological changes in the poikilocytes in terms of the population reductions or elevation in comparison with set controls. These morphologies may be due to morphological alteration of protein molecules which form the framework of erythrocytes [13,14]. Free radicals are usually present in the body as active substances in various physiological processes.…”

Section: Resultsmentioning

confidence: 99%

Impacts of laser energy doses in maintaining the shape and deformability of the red blood cell (morphological &physiological) in vitro

2020

Lett Appl NanoBioSci

View full text Add to dashboard Cite

Lasers have been applied in various clinical procedures such as surgery and cardiology to clear occluded blood vessels. However, there subsists insight into the relevant parameters and their ability to develop a rheological property of blood. Two blood samples were used. Morphological changes that have been chosen to detect this effect on red cells were: echinocytes, dacrocytes, stomatocytes, keratocytes , schistocytes. Laser beams λ = 532nm , power 100 mw at different intervals (0, 1, 3, 6, and 10 seconds).Six slides prepared for each interval. Slides were irradiated and microscopic photographs were taken to observe changes in population of each poikilocytes were compared to non-irradiated control blood smears. The effects were listed in a table in the form of a scale. Various laser energy doses were able to reduce the population of some poikilocytes namely ( dacrocytes, stomatocytes) in a male and (echinocytes, dacrocytes, stomatocytes, keratocytes and schistocytes) in a female by one, two or three cells. A poikilocyte is a distorted red blood cell and bears different forms, for instance, created (echinocytes), teardrop (dacrocytes), bite cells (keratocytes) or even fragmented (schistocytes). Low-energy laser improves the monographic properties of red cells by enhancing ATPase activity, metabolic output, activity of antioxidant enzymes.

show abstract

Section: Resultsmentioning

confidence: 99%

Impacts of laser energy doses in maintaining the shape and deformability of the red blood cell (morphological &physiological) in vitro

2020

Lett Appl NanoBioSci

View full text Add to dashboard Cite

show abstract

“…However, important spectral regions are indicated by positions 7 and 8, which are combination bands involving O-H stretch and O-H bend, respectively. (Baddini et al 2010) The predicted values of fat, protein and moisture content in the external validation set samples are shown in Table 3. Samples one and two are actually the same ricotta sample with spectra obtained from two different parts of the analyzed surface.…”

Section: Resultsmentioning

confidence: 99%

Determination of fat, protein and moisture in ricotta cheese by near infrared spectroscopy and multivariate calibration

2013

View full text Add to dashboard Cite

Near-infrared reflectance spectroscopy (NIRS) with partial least squares regression (PLSR) was used to determine levels of fat, protein and moisture in ricotta cheese without complex sample preparation. Spectra of 19 conventional and low-fat ricotta samples from different manufacturers were collected in duplicate, with 33 of the 38 spectra used as a calibration set and the remaining 5 spectra used as an external validation set. The best results were obtained by processing the spectral region between 1,100 and 2,500 nm. Multivariate models with six latent variables (LVs) showed good prediction capability for fat and protein determinations, with average relative errors (Er) of 6.37 % and 5.95 %, respectively. For moisture, a more robust model was obtained with 4 LVs, showing better prevision capacity and Er of 1.91 %.

show abstract

“…The infrared spectral feature of blood samples is severely overlapped and broad without distinct signature of individual components. The diagnosis of hyperlipidemia by infrared spectroscopy is feasible, but its predictive accuracy is not high enough and needs further improvement . Fourier transform infrared (FTIR) spectroscopy analysis has the advantage of higher instrument stability, depth of light penetration and predictive capacity of some quality characteristics .…”

Section: Introductionmentioning

confidence: 99%

“…The diagnosis of hyperlipidemia by infrared spectroscopy is feasible, but its predictive accuracy is not high enough and needs further improvement. 9,10 Fourier transform infrared (FTIR) spectroscopy analysis has the advantage of higher instrument stability, depth of light penetration and predictive capacity of some quality characteristics. 11,12 FTIR technology has been widely used in fields of biomedicine, environment, agriculture, food, etc.…”

Section: Introductionmentioning

confidence: 99%

Use of random forest in FTIR analysis of LDL cholesterol and tri‐glycerides for hyperlipidemia

Chen

Tang

et al. 2015

Biotechnology Progress

View full text Add to dashboard Cite

A quantitative determination method for the diagnosis of hyperlipidemia was developed using Fourier transform infrared (FTIR) spectroscopy. Random forest (RF) was demonstrated as a potential multivariate algorithm for the FTIR analysis of low-density lipoprotein cholesterol (LDL-C) and tri-glycerides (TG) in human serum samples. The informative wavebands for LDL-C and TG were selected based on the Gini importance. The selected wavebands were mainly within the fingerprint region. The RF modeling results were better than those derived using PLS in validation process, because the chance for over-fitting was possibly eliminated in RF algorithm. ARF also demonstrated favorable results in the test process. The prospective model exhibited a higher than 90% true prediction in negative/positive properties for male and female samples. These clinical statistical results indicated the optimization of RF algorithm performed accurately in the FTIR determination of LDL-C and TG. RF is evaluated as a promising tool for diagnosing and controlling hyperlipidemia in populations. The parameter optimization methodology is useful in the improving model accuracy using FTIR spectroscopic technology.

show abstract

Determination of total protein in hyperimmune serum samples by near-infrared spectrometry and multivariate calibration

Cited by 13 publications

References 20 publications

Impacts of laser energy doses in maintaining the shape and deformability of the red blood cell (morphological &physiological) in vitro

Impacts of laser energy doses in maintaining the shape and deformability of the red blood cell (morphological &physiological) in vitro

Determination of fat, protein and moisture in ricotta cheese by near infrared spectroscopy and multivariate calibration

Use of random forest in FTIR analysis of LDL cholesterol and tri‐glycerides for hyperlipidemia

Contact Info

Product

Resources

About