Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategy

Pebdeni, Azam Bagheri; Mousavizadegan, Maryam; Hosseini, Morteza

doi:10.1016/j.foodchem.2021.130137

Cited by 43 publications

(7 citation statements)

References 43 publications

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“…However, a noticeable red shift in the former peak (around 3400 cm –1 ) was observed compared to free BSA at around 3305 cm –1 , attributed to potential interactions between Mn 2+ and the −NH, −OH groups of BSA . Furthermore, upon conjugation of vancomycin to BSA-MnO 2 , an additional peak emerged at 1230 and 1063 cm –1 (purple arrows), corresponding to the aromatic phenol and C–O–C in vancomycin, respectively. , The infrared results confirm the successful preparation of the Van@BSA-MnO 2 complex.…”

Section: Resultsmentioning

confidence: 99%

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Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme

Ma,

Lin,

Xue

et al. 2024

Anal. Chem.

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Pathogenic bacterial infections, even at extremely low concentrations, pose significant threats to human health. However, the challenge persists in achieving high-sensitivity bacterial detection, particularly in complex samples. Herein, we present a novel sandwich-type electrochemical sensor utilizing bacteria-imprinted polymer (BIP) coupled with vancomycinconjugated MnO 2 nanozyme (Van@BSA-MnO 2 ) for the ultrasensitive detection of pathogenic bacteria, exemplified by Staphylococcus aureus (S. aureus). The BIP, in situ prepared on the electrode surface, acts as a highly specific capture probe by replicating the surface features of S. aureus. Vancomycin (Van), known for its affinity to bacterial cell walls, is conjugated with a Bovine serum albumin (BSA)-templated MnO 2 nanozyme through EDC/NHS chemistry. The resulting Van@BSA-MnO 2 complex, serving as a detection probe, provides an efficient catalytic platform for signal amplification. Upon binding with the captured S. aureus, the Van@BSA-MnO 2 complex catalyzes a substrate reaction, generating a current signal proportional to the target bacterial concentration. The sensor displays remarkable sensitivity, capable of detecting a single bacterial cell in a phosphate buffer solution. Even in complex milk matrices, it maintains outstanding performance, identifying S. aureus at concentrations as low as 10 CFU mL −1 without requiring intricate sample pretreatment. Moreover, the sensor demonstrates excellent selectivity, particularly in distinguishing target S. aureus from interfering bacteria of the same genus at concentrations 100-fold higher. This innovative method, employing entirely synthetic materials, provides a versatile and low-cost detection platform for Gram-positive bacteria. In comparison to existing nanozyme-based bacterial sensors with biological recognition materials, our assay offers distinct advantages, including enhanced sensitivity, ease of preparation, and cost-effectiveness, thereby holding significant promise for applications in food safety and environmental monitoring.

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Section: Resultsmentioning

confidence: 99%

“…22 Furthermore, upon conjugation of vancomycin to BSA-MnO 2 , an additional peak emerged at 1230 and 1063 cm −1 (purple arrows), corresponding to the aromatic phenol and C−O−C in vancomycin, respectively. 25,26 The infrared results confirm the successful preparation of the Van@BSA-MnO 2 complex.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme

Ma,

Lin,

Xue

et al. 2024

Anal. Chem.

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“…Obliosca et al reported that silver nanoclusters (AgNCs) could be used as fluorescent nanomaterials for the sensitive and specific detection of DNA [ 63 ]. Copper nanoclusters (CuNCs) are also used in food monitoring, and Bagheri et al proposed a dual detection sensor using aptamer- and antibiotic-based methods combined with CuNCs [ 64 ] as an effective method for the identification and quantification of Staphylococcus aureus ( S. aureus ) pathogens. When S. aureus was used as a specific target to induce the aggregation of CuNC-labeled aptamer, the interaction between them limited the internal movement of molecules and provided energy for luminescence.…”

Section: Nanomaterials In Food Safety Analysismentioning

confidence: 99%

Nanomaterial-Based Fluorescent Biosensor for Food Safety Analysis

Zhou

Gui

et al. 2022

Biosensors

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Food safety issues have become a major threat to public health and have garnered considerable attention. Rapid and effective detection methods are crucial for ensuring food safety. Recently, nanostructured fluorescent materials have shown considerable potential for monitoring the quality and safety of food because of their fascinating optical characteristics at the nanoscale. In this review, we first introduce biomaterials and nanomaterials for food safety analysis. Subsequently, we perform a comprehensive analysis of food safety using fluorescent biosensors based on nanomaterials, including mycotoxins, heavy metals, antibiotics, pesticide residues, foodborne pathogens, and illegal additives. Finally, we provide new insights and discuss future approaches for the development of food safety detection, with the aim of improving fluorescence detection methods for the practical application of nanomaterials to ensure food safety and protect human health.

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“…One of the most pathogenic bacteria is Staphylococcus aureus (S. aureus) that have a strong potential to food poisoning and causes various diseases and infections in human. [1][2] Endotoxins and other characteristics of these bacteria have made them a significant threat to the human health causing infections and complications. [3] Constructing accurate and sensitive detection methods for S. aureus has become an important topic.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor for Selective and Sensitive Detection of Staphylococcus aureus Using Graphene oxide/poly Deep Eutectic Solvent/Nickel Oxide Nanocomposite Modified Electrode

Rabiei,

Rezayan,

Hosseini

et al. 2024

ChemistrySelect

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Since that pathogenic bacteria are major threats to human health; this research describes the fabrication of an effective and sensitive platform based on graphene oxide/poly deep eutectic solvent/nickel oxide nano particles (GO/PDES/NiO NPs) nanocomposite at the surface of glassy carbon electrode for selective detection of Staphylococcus aureus (S. aureus). The DNA aptamers were immobilized on the modified electrode through the EDC/NHS chemistry. Each aptasensor modification step was checked by with cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) techniques. The linear dynamic range of the proposed electrochemical aptasensor covered a large variation of S. aureus concentrations from 101 – 108 CFU.mL−1 and a detection limit of 10 CFU.mL−1 under optimum conditions was obtained. Analysis of spiked tap water, lake water and human serum samples revealed high potential of this aptasensor as a sensitive, effective, and label‐free promising tool for S. aureus detection. This is the first time that poly deep eutectic solvents used in live bacteria detection.

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Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategy

Cited by 43 publications

References 43 publications

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme

Nanomaterial-Based Fluorescent Biosensor for Food Safety Analysis

Electrochemical Aptasensor for Selective and Sensitive Detection of Staphylococcus aureus Using Graphene oxide/poly Deep Eutectic Solvent/Nickel Oxide Nanocomposite Modified Electrode

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Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategy

Cited by 43 publications

References 43 publications

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO2 Nanozyme

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO2 Nanozyme

Nanomaterial-Based Fluorescent Biosensor for Food Safety Analysis

Electrochemical Aptasensor for Selective and Sensitive Detection of Staphylococcus aureus Using Graphene oxide/poly Deep Eutectic Solvent/Nickel Oxide Nanocomposite Modified Electrode

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Product

Resources

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Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme

Ultrasensitive and Highly Selective Detection of Staphylococcus aureus at the Single-Cell Level Using Bacteria-Imprinted Polymer and Vancomycin-Conjugated MnO₂ Nanozyme