Sensitive determination of bromhexine hydrochloride based on its quenching effect on luminol/H₂O₂ electrochemiluminescence system

Abstract

In this paper, the electrochemiluminescence (ECL) behavior of luminol/H O system in the presence of bromhexine hydrochloride (BrH) was investigated. It was found that the ECL intensity of luminol/H O system on a platinum electrode could be intensely quenched by BrH owing to the scavenging superoxide radical ability of BrH, and therefore the sensitive determination of BrH was possible. Under optimal conditions, the quenched ECL intensity was linear to the concentration of BrH in a wide range of 0.08 to 500 μM, with a detection limit of 0.02 μM (signal-to-noise ratio (S/N) = 3). This ECL method possessed the merits of rapid, simple and sensitive, and was successfully applied to the BrH quantification in pharmaceutical preparations with satisfactory recoveries of 91.0 ± 4.0 to 106.5 ± 3.4%. The possible route of the quenched ECL of luminol/H O in the presence of BrH was also discussed.

Preparation of a New Molecularly Imprinted Polymers and Its Use in the Selective Extraction for Determination Bromhexine Hydrochloride at Pharmaceuticals

“…According to review, bromhexine hydrochloride was determination by several methods such as these recent methods that employed reverse phase-high performance liquid chromatography (16,19) , spectrophotometry (14) , potentiometric-flow injection (11) , electrochemiluminescence system (12) , TLC densitometric (18) . Several techniques used for determination drugs and pharmaceuticals.…”

CFIA-ISNAG fluorimeter for the Determination of Bromhexine-HCl in Drugs via the Measurement Scattered Light at ± 90˚

“…Furthermore, BH-HCl is one of the mucous-modifying drugs that aid expectoration by improving the flow characteristics of bronchial mucous [3,4]. A literature review indicated the determination of bromhexine hydrochloride in dosage form, either in combined form or alone, by spectrophotometric [5][6][7][8][9][10][11], potentiometric [12], voltammetric [13], colorimetric [14], chemiluminescence [15,16], TLC [17], HPLC [18][19][20][21][22][23][24][25], RRLC-MS/MS [26], and HPTLC-HPLC [27]. This study presents a new method to determine BH-HCl in pharmaceutical formulations using tetraphenylborate as a precipitating reagent to form a yellowish-white precipitate with the use of a homemade instrument.…”

Electrodeposition of Cobalt Oxides on Carbon Nanotubes for Sensitive Bromhexine Sensing

“…Thus, the accurate quantification of BHC in pharmaceutical products and clinical samples is critical [4]. Several analytical techniques have been employed for BHC analysis, including high-performance liquid chromatography (HPLC) [5], spectrophotometry [6], electrochemiluminescence [7], and potentiometric titration [8]. These methods require sophisticated and expensive instruments, which are often not available in low-resource areas.…”