Sensitive determination of four camptothecins by solid-phase microextraction-HPLC based on a boronic acid contained polymer monolithic layer

Chen, Jishun; Min, Xinwen; Li, Peng; Wu, Chen; Tian, Dawei; Chen, Qinhua

doi:10.1016/j.aca.2015.03.058

Cited by 17 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The crown ether polymers doped in the nanofibers can form a host-guest complex with the guest molecules containing amine groups by H-bonds, such as the catecholamines in this paper. In addition, Hongyou Hu et al and Jishun Chen et al also proposed that boronic acid can bond with nitrogen atoms in the chemical structure through B-N interactions, and that the hydrophobic skeleton can interact with benzene rings and other aliphatic groups of analytes 36,37 . Also, during the desorption process, the hydrogen bonds and B-N interactions are easily broken at very low pH; thus, eluants with acidic pH are usually suitable for exploration for desorption.…”

Section: Discussionmentioning

confidence: 99%

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Xie¹,

Chen²,

Gu³

et al. 2018

JoVE

View full text Add to dashboard Cite

The extraction and analysis of catecholamine neurotransmitters in biological fluids is of great importance in assessing nervous system function and related diseases, but their precise measurement is still a challenge. Many protocols have been described for neurotransmitter measurement by a variety of instruments, including high-pressure liquid chromatography (HPLC). However, there are shortcomings, such as complicated operation or hard-to-detect multiple targets, which cannot be avoided, and presently, the dominant analysis technique is still HPLC coupled with sensitive electrochemical or fluorimetric detection, due to its high sensitivity and good selectivity. Here, a detailed protocol is described for the pretreatment and detection of catecholamines with high pressure liquid chromatography with electrochemical detection (HPLC-ECD) in real urine samples of infants, using electrospun composite nanofibers composed of polymeric crown ether with polystyrene as adsorbent, also known as the packed-fiber solid phase extraction (PFSPE) method. We show how urine samples can be easily precleaned by a nanofiber-packed solid phase column, and how the analytes in the sample can be rapidly enriched, desorbed, and detected on an ECD system. PFSPE greatly simplifies the pretreatment procedures for biological samples, allowing for decreased time, expense, and reduction of the loss of targets. Overall, this work illustrates a simple and convenient protocol for solid-phase extraction coupled to an HPLC-ECD system for simultaneous determination of three monoamine neurotransmitters (norepinephrine (NE), epinephrine (E), dopamine (DA)) and two of their metabolites (3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxy-phenylacetic acid (DOPAC)) in infants' urine. The established protocol was applied to assess the differences of urinary catecholamines and their metabolites between high-risk infants with perinatal brain damage and healthy controls. Comparative analysis revealed a significant difference in urinary MHPG between the two groups, indicating that the catecholamine metabolites may be an important candidate marker for early diagnosis of cases at risk for brain damage in infants.

show abstract

Section: Discussionmentioning

confidence: 99%

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Xie¹,

Chen²,

Gu³

et al. 2018

JoVE

View full text Add to dashboard Cite

show abstract

“…Due to these exclusive characteristics, polymers containing boronic acid have found various applications, including the selective recognition of glycoproteins 4,5 and saccharides, 6 as insoluble supports for solid-phase synthesis, 7 as agents for the Suzuki reaction, 8 and as materials for solid-phase microextraction. 9,10 The equilibrium between boronate and boronic acid has recently been widely taken advantage of producing frameworks of polymeric compounds with special properties. This equilibrium, also conceptualized as involving dynamic covalent bonds, is the foundation of various responsive materials, that is materials that interact with and selectively respond to the environment for various purposes, such as the specific delivery of cargo.…”

Section: Introductionmentioning

confidence: 99%

Accelerated hydrolysis of boronic acid in a modified poly(amidoamine) dendrimer: identification of a factor leading to the production of an impurity in boronic acid containing poly(amidoamine) dendrimers

et al. 2017

View full text Add to dashboard Cite

The preparation of boronic acid containing dendrimers is still a difficult task in dendrimer chemistry. In this investigation, an unanticipated hydrolysis product (4) was identified during the preparation of (G:2)-PAMAM-dendri-(4-phenyl boronic acid)14 (3) by acquiring its 1H NMR, 13C NMR, and UV–vis absorption spectra and IR spectra and comparing them with those of 4-hydroxylbenzoic acid (5). Furthermore, an Alizarin Red S staining analysis, ICP-MS, and 11B NMR spectrum indicated the lack of boronic acid in 4. Finally, treatment of 3 with hydrogen peroxide gave a product that was determined to be identical to dendrimer 4. By analyzing the formation of 4 under various conditions, the presence of numerous primary amines in a PAMAM dendrimer did accelerate the hydrolysis of peripheral boronic acid. This investigation revealed an apparent intrinsic problem that needs to be overcome during the preparation of boronic acid containing dendrimers and related boronic acid containing macromolecules.

show abstract

Boronate-modified hollow molecularly imprinted polymers for selective enrichment of glycosides

Xia

Huang

et al. 2017

Microchim Acta

View full text Add to dashboard Cite

D-Glucose was used as a dummy template in a strategy to fabricate boronate-based hollow molecularly imprinted polymers (h-MIPs) for the recognition of glycosides. 3-Aminophenylboronic acid (APBA) was selected as the functional monomer to impart glycoside binding capability. A polystyrene core was synthesized via an emulsifier-free emulsion polymerization. It was then be corroded to form a hollow structure where the binding sites on the inner surface can be fully utilized. Adsorption studies indicate that the resulting h-MIPs can specifically recognize glycosides. The h-MIPs were applied as a sorbent for solid phase extraction of the glycosides (daidzin, glycitin, rutin, and genistin) in soy products. Following desorption with 1% TFA in acetonitrile-water (3:7, v/v), the glycosides were quantified by HPLC with UV detection. The detection limits for the glycosides range from 3.5 to 10.8 ng·mL. Graphical abstract Boronate-affinity hollow molecularly imprinted polymers using D-glucose as dummy template were prepared as solid phase extraction adsorbents for selective enrichment of glycosides in soy products prior to quantitation by HPLC-UV.

show abstract

Sensitive determination of four camptothecins by solid-phase microextraction-HPLC based on a boronic acid contained polymer monolithic layer

Cited by 17 publications

References 25 publications

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Accelerated hydrolysis of boronic acid in a modified poly(amidoamine) dendrimer: identification of a factor leading to the production of an impurity in boronic acid containing poly(amidoamine) dendrimers

Boronate-modified hollow molecularly imprinted polymers for selective enrichment of glycosides

Contact Info

Product

Resources

About