Sensitive determination of sialic acid expression on living cells by using an ITO electrode modified with graphene, gold nanoparticles and thionine for triple signal amplification

Qian, Dongping; Han, Fengfeng; Li, Weibo; Bao, Ning; Yu, Chunmei; Gu, Hongchen

doi:10.1007/s00604-017-2390-1

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…Two-dimensional NS materials, atomically thin sheets, have attracted tremendous interest due to their fascinating properties [ 52 , 53 , 54 , 55 , 56 , 57 , 58 ]. Inspired by the huge success of graphene and black phosphorus, a series of 2D materials have been exploited and demonstrated to have great potential in many applications, such as energy [ 8 , 59 , 60 , 61 ], catalysis [ 48 , 62 , 63 , 64 ], sensors [ 65 , 66 , 67 , 68 ], nanophotonics [ 9 , 58 , 69 , 70 , 71 , 72 ], and biomedicines [ 73 , 74 , 75 ]. Although great progress in 0D and 1D PbO nanostructures has been achieved, much less is known about 2D PbO NSs, their synthetic strategies, and their fascinating performance so far.…”

Section: Synthesis Of Pbo Nanostructuresmentioning

confidence: 99%

Lead Monoxide Nanostructures for Nanophotonics: A Review

2023

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Black-phosphorus-analog lead monoxide (PbO), as a new emerging 2D material, has rapidly gained popularity in recent years due to its unique optical and electronic properties. Recently, both theoretical prediction and experimental confirmation have revealed that PbO exhibits excellent semiconductor properties, including a tunable bandgap, high carrier mobility, and excellent photoresponse performance, which is undoubtedly of great interest to explore its practical application in a variety of fields, especially in nanophotonics. In this minireview, we firstly summarize the synthesis of PbO nanostructures with different dimensionalities, then highlight the recent progress in the optoelectronics/photonics applications based on PbO nanostructures, and present some personal insights on the current challenges and future opportunities in this research area. It is anticipated that this minireview can pave the way to fundamental research on functional black-phosphorus-analog PbO-nanostructure-based devices to meet the growing demands for next-generation systems.

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Section: Synthesis Of Pbo Nanostructuresmentioning

confidence: 99%

Lead Monoxide Nanostructures for Nanophotonics: A Review

2023

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“…The DPV based electrochemical method was used to monitor the changes in expression of Sia in response to addition of sialidase enzyme. 525 A similar approach combined 3-aminophenylboronic acid modified carbon nanospheres for Sia recognition with horseradish peroxidase decorated gold nanoparticles for signal amplification. The electrochemical cytosensor realised an identification system using Sambucus nigra agglutinin and 3-aminophenylboronic acid modified carbon nanospheres for cancer cell capture with measurement of the expression level of Sia on MCF-7 cells vs. control cell lines (LOD of 25 cells per mL).…”

Section: Indirect Utilization Of Sia In Cytosensing Therapy and Imagi...mentioning

confidence: 99%

Explorations in a galaxy of sialic acids: a review of sensing horizons, motivated by emerging biomedical and nutritional relevance

2022

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“…5,6 SA usually presents on the cell surface as sialoglycoconjugates, such as sialylated proteins and lipids. 7 Traditional SA sensing strategies, including mass spectrometry, 8 plasmon resonance, 9,10 colorimetric methods, 11,12 liquid chromatography, 13 electrochemical sensing, 14,15 and fluorescence analysis 16−20 have limitations for in situ analysis applications. The most frequently used SA analysis approach focuses on the separation and extraction of SA-terminated glycopeptides, enzymolysis, and liquid chromatography-mass spectrometric analysis.…”

mentioning

confidence: 99%

“…It is known to be an indicator for some cancers owing to its overexpression on cell surfaces. − SA on the outermost cell surface is involved in cellular communication and the key points of the immune system, which has been studied for exploitation in tumor therapies. , SA usually presents on the cell surface as sialoglycoconjugates, such as sialylated proteins and lipids . Traditional SA sensing strategies, including mass spectrometry, plasmon resonance, , colorimetric methods, , liquid chromatography, electrochemical sensing, , and fluorescence analysis − have limitations for in situ analysis applications. The most frequently used SA analysis approach focuses on the separation and extraction of SA-terminated glycopeptides, enzymolysis, and liquid chromatography-mass spectrometric analysis. , …”

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confidence: 99%

In Situ Determination of Sialic Acid on Cell Surface with a pH-Regulated Polymer Enzyme Nanoreactor

et al. 2021

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Sialic acid (SA) is an important monosaccharide that is involved in incurable cancer immunotherapy. However, it is difficult to detect SA in situ using the existing strategy based on the SA-terminated glycopeptide extraction from the cell lysate. The countermeasures of the bottleneck caused by cell disruption and peptide extraction should be designed based on a “cell-surface attachment and controlled enzymolysis” protocol. Herein, a poly(styrene-co-maleic anhydride-acrylic acid-concanavalin A) (PSM-PAA-ConA) was synthesized and developed as a pH-regulated enzyme nanoreactor after being loaded with sialidase and myoglobin. The nanoreactor showed controllable biocatalysis induced by a cascade enzyme reaction and applied for the in situ detection of SA on a living cell surface. The addition of an acidic solution resulted in a decrease in the size of the nanoreactor and enhancement of its permeability, triggering an “on” state of the SA catalysis. Subsequent pH increase led to increased hydrophilicity of the nanoreactor, increasing its size and resulting in the catalytic “off” state. ConA assisted the cell-surface attachment of the enzyme reactor. Furthermore, SA on the surface of living cancer cells was successfully monitored by the pH-regulated enzyme nanoreactor, demonstrating the feasibility of high specificity in situ analysis for SA. This pH-induced catalytic efficiency control by the enzyme nanoreactor provides a potential platform for functional stimuli-responsive catalytic systems as well as a strategy for in situ analysis of biomolecules on the cell surface.

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Sensitive determination of sialic acid expression on living cells by using an ITO electrode modified with graphene, gold nanoparticles and thionine for triple signal amplification

Cited by 10 publications

References 31 publications

Lead Monoxide Nanostructures for Nanophotonics: A Review

Lead Monoxide Nanostructures for Nanophotonics: A Review

Explorations in a galaxy of sialic acids: a review of sensing horizons, motivated by emerging biomedical and nutritional relevance

In Situ Determination of Sialic Acid on Cell Surface with a pH-Regulated Polymer Enzyme Nanoreactor

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