Sensitive Dual Electrochemical-Colorimetric Point-of-Care (POC) Sensor for the Rapid Detection of Mycoplasma pneumoniae

A LAMP-based hydrogen ion selective electrochemical sensor for highly sensitive detection of Mycoplasma pneumoniae

“…38 Stability represents the sensor's ability to maintain consistent performance during continuous use and the extent of performance drift over time. 7,39 These parameters play a critical role in assessing the performance of electrochemical sensors and determining their effectiveness and reliability in practical applications. Therefore, this study focuses on testing these performance aspects of the H + -LAMP sensor.…”

“…In the LAMP reaction solution, additional interfering ions are present besides the target ion under test. 7 To verify their potential impact on pH determination, the electrode response of a pH 7.6 PBS buffer was individually tested with final concentrations of 20 mM Na + , 20 mM SO 4 2− , 6 mM Mg 2+ , 20.0 mM NH 4 + , 10 mM K + , and 10 mM Cl − . This verification aimed to assess the sensor's interference resistance.…”

“…5 Currently, common methods for detecting M. pneumoniae include nucleic acid amplification tests (NAATs) and serological diagnosis. 6,7 Serological diagnosis is time-consuming, demanding precise experimental conditions and sophisticated instruments, making it unsuitable for home or primary healthcare settings. 1…”

“…Electrochemical detection is considered as an effective alternative to NAATs due to its potential for miniaturization and simplified operation. 7,13–16 Concurrently, the integration of isothermal nucleic acid amplification with electrochemical detection is gaining attention as a newly emerging detection technology. 17–19 Nevertheless, these methods often require additional electrode incubation, post-amplification product disposal or complex electrochemical workstations.…”