Sensitive Hyaluronidase Biosensor Based on Target-Responsive Hydrogel Using Electronic Balance as Readout

Abstract

The development of simple but sensitive methods for hyaluronidase (HAase) detection has been paid a great deal of attention because HAase is a potential cancer marker. In this work, a novel system coupled with a controlled release system has been designed for HAase determination without complex analytical instruments and skilled technicians. Pt@SiO 2 nanoparticles (NPs), which can catalyze the breakdown of H 2 O 2 into O 2 and H 2 O, was embedded in the hydrogel constructed by polyethylenimine (PEI) and hyaluronic acid (HA). In the presence of HAase, the hydrogel was broken down as HAase can catalyze the degradation of HA and hence the Pt@SiO 2 NPs in the hydrogel was released. The released Pt@SiO 2 NPs mixed with H 2 O 2 solution in a drainage device, and then O 2 was generated due to the decomposition of H 2 O 2 , resulting in an enhancement of pressure in the drainage device because of the low solubility of O 2 . A certain amount of H 2 O overflowed from the drainage device because the difference of the pressure between the inner and outer of the drainage device. The overflowed H 2 O was collected by a tube, and its amount was easily measured by an electronic balance. The weight of the H 2 O has a linear relationship with the HAase concentration in the range of 1−60 U/mL (120 min enzymatic hydrolysis time) and 0.2−10 U/mL (240 min enzymatic hydrolysis time). The developed system has been applied to detect the activity of HAase in urine samples with satisfied results.

Hydrogel‐based composites: Unlimited platforms for biosensors and diagnostics

“…Up to now, hydrogels have been composited with a wide range of functional materials, and depending on the type of filler materials, they are classified as hydrogel–biomacromolecule composites, [ 69–73 ] hydrogel–metal nanostructure composites, [ 74–82 ] hydrogel–carbon nanomaterial composites, [ 28,31,34,36 ] and hydrogel–2D material beyond graphene composites (Figure 2). [ 83–89 ]…”

“…Noble metal nanostructures (NSs) such as nanoparticles (NPs), nanowires (NWs), and thin films of Au, Ag, or Pt are popular candidates in the formation of composites with hydrogels. [ 66,74–82,129–133 ] Their introduction into the structures of hydrogels endows the composites with additional functional properties, such as optical, [ 15,16 ] electrical, [ 17–19 ] and catalytic properties. [ 21–25,40 ] Generally speaking, hydrogel–metal NS composites can be prepared by either deposition of metal structures on/into a pre‐formed hydrogel or by in situ polymerization of a hydrogel in the presence of presynthesized metal NSs.…”

Synthesis of enzymatic hydrogels and their application in biosensors

“…图 22 基于双发夹分子信标(DHMB)的比色生物传感器用 于K-Ras DNA扩增检测的原理图 [53] (网络版彩图) [56] (网络版彩图) 图 24 酶促单宁酸聚合及其修饰多种电极表面应用原理 图 [55] (网络版彩图) 图 23 基于金纳米粒子作为淬灭源的p53基因检测GNP-HPP传感系统原理图 [54] (网络版彩图) 图 26 检测HAase的水凝胶生物传感器原理图 [57] (网络版 彩图)…”

The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering

“…Additionally, the hydroxyl group and carboxyl group (via the intermolecular bonds and intramolecular bonds that exist between them) can form self-cross-linking polymers, such as ACPTM, after esterification in vivo. ese hydrogels have been used to reduce postoperative adhesions and have served as scaffolds for cell growth and repair in bone tissue defects [11].…”

“…e biocompatibility of synthetic polymer hydrogels needs improvement. ere have been several in-depth studies of the natural polymer HA [9][10][11][12][13]. As a constituent of the natural cytoplasmic matrix, favorable biological activity of HA and its ability to communicate with cells are undisputed.…”