Sensitive label-free immunoglobulin G detection using a MEMS quartz crystal microbalance biosensor with a 125 MHz wireless quartz resonator

Zhou, Lianjie; Kato, Fumihito; Ogi, Hirotsugu

doi:10.35848/1347-4065/abea50

Cited by 20 publications

(18 citation statements)

References 43 publications

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“…Antibodies can be easily detected by a piezoelectric biosensor. This type of assay was described for the diagnosis of various pathological states with quartz crystal microbalance as the sensor platform [ 94 , 95 , 96 , 97 ]. This type of biosensor remains rare for development of new point-of-care tests for the diagnosis, but they are quite convenient for the purpose and interest for piezoelectric platform can grow in the future.…”

Section: Biosensors For Measuring Anti-covid-19 Antibodiesmentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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Coronavirus disease 2019 (COVID-19) is a highly virulent infection that has caused a pandemic since 2019. Early diagnosis of the disease has been recognized as one of the important approaches to minimize the pathological impact and spread of infection. Point-of-care tests proved to be substantial analytical tools, and especially lateral flow immunoassays (lateral flow tests) serve the purpose. In the last few years, biosensors have gained popularity. These are simple but highly sensitive and accurate analytical devices composed from a selective molecule such as an antibody or antigen and a sensor platform. Biosensors would be an advanced alternative to current point-of-care tests for COVID-19 diagnosis and standard laboratory methods as well. Recent discoveries related to point-of-care diagnostic tests for COVID-19, the development of biosensors for specific antibodies and specific virus parts or their genetic information are reviewed.

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Section: Biosensors For Measuring Anti-covid-19 Antibodiesmentioning

confidence: 99%

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Pohanka

2022

Sensors

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“…The conventional QCM sensor consists of a quartz plate (or resonator) with electrodes on both sides to which wires are connected. The electrodes cause significant inertia resistance at the resonator surfaces due to much larger mass density than that of quartz, deteriorating the mass sensitivity of QCM. , In addition, electric connectors on the quartz resonator for fixation, power supply, and signal detection not only deteriorate the quality factor of the resonator but also limit the use of a resonator with high fundamental resonant frequency (< ∼10 MHz). − To improve the operating frequency and thus the sensitivity of QCM, the wireless and electrodeless QCM has been developed using the microelectro-mechanical-systems (MEMS) processes. − Instead of using the contacting electrodes, excitation and detection of the shear vibration are achieved by noncontacting antennas in the wireless QCM. Although Au films were occasionally used for forming a self-assembled monolayer through the alkanethiol reaction, their thickness was only several nanometers, affecting little QCM’s mass sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…We previously developed a wireless MEMS QCM biosensor, where the excitation of quartz resonator and signal readout are performed by the antennas outside the sensor chip . The long fluid microchannel resulted in a large sensor chip with 20 mm , or 10 mm length. The relatively long distance between the quartz resonator and the antennas deteriorates the signal-to-noise ratio.…”

Section: Introductionmentioning

confidence: 99%

Mass-Fabrication Scheme of Highly Sensitive Wireless Electrodeless MEMS QCM Biosensor with Antennas on Inner Walls of Microchannel

et al. 2023

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Quartz-crystal-microbalance (QCM) biosensor is a typical label-free biosensor, and its sensitivity can be greatly improved by removing electrodes and wires that would be otherwise attached to the surfaces of the quartz resonator. The wireless-electrodeless QCM biosensor was then developed using a microelectro-mechanical systems (MEMS) process, although challenges remain in the sensitivity, the coupling efficiency, and the miniaturization (or mass production). In this study, we establish a MEMS process to obtain a large number of identical ultrasensitive and highly efficient sensor chips with dimensions of 6 mm square. The fundamental shear resonance frequency of the thinned AT-cut quartz resonator packaged in the microchannel exceeds 160 MHz, which is excited by antennas deposited on inner walls of the microchannel, significantly improving the electro-mechanical coupling efficiency in the wireless operation. The high sensitivity of the developed MEMS QCM biosensors is confirmed by the immunoglobulin G (IgG) detection using protein A and ZZ-tag displaying a bionanocapsule (ZZ-BNC), where we find that the ZZ-BNC can provide more effective binding sites and higher affinity to the target molecules, indicating a further enhancement in the sensitivity of the MEMS QCM biosensor. We then perform the label-free C-reactive protein (CRP) detection using the ZZ-BNC-functionalized MEMS QCM biosensor, which achieves a detection limit of 1 ng mL–1 or less even with direct detection.

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“…In recent years, ultrasound technology has made remarkable progress in medical applications, and is often used for imaging, 1, 2 biosensors, [3][4][5] and gas sensors, 6,7 and the ultrasound frequencies used in these applications are generally in the megahertz band. However, it has become clear that even low-frequency ultrasound waves with a frequency of several tens of kilohertz can interact with small proteins and dramatically affect their reactions as described below.…”

Section: Introductionmentioning

confidence: 99%

Acceleration of amyloid fibril formation by multichannel sonochemical reactor

Noi,

Nakajima,

Yamaguchi

et al. 2021

Preprint

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Formation of amyloid fibrils of various amyloidogenic proteins is dramatically enhanced by ultrasound irradiation. For applying this phenomenon to the study of protein aggregation science and diagnosis of neurodegenerative diseases, a multichannel ultrasound irradiation system with individually adjustable ultrasound-irradiation conditions is necessary. Here, we develop a sonochemical reaction system, where an ultrasonic transducer is placed in each well of a 96-well microplate to perform ultrasonic irradiation of sample solutions under various conditions with high reproducibility, and applied it for studying amyloidfibril formation of amyloid β, α-synuclein, β2-microglobulin, and lysozyme. The results clearly show that our instrument is superior to conventional shaking method in terms of degree of acceleration and reproducibility of fibril formation reaction. The acceleration degree is controllable by controlling the driving voltage applied to each transducer. We have thus succeeded in developing a useful tool for the study of amyloid fibril formation in various proteins.

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Sensitive label-free immunoglobulin G detection using a MEMS quartz crystal microbalance biosensor with a 125 MHz wireless quartz resonator

Cited by 20 publications

References 43 publications

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Progress in Biosensors for the Point-of-Care Diagnosis of COVID-19

Mass-Fabrication Scheme of Highly Sensitive Wireless Electrodeless MEMS QCM Biosensor with Antennas on Inner Walls of Microchannel

Acceleration of amyloid fibril formation by multichannel sonochemical reactor

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