Sensitive optical detection of clinically relevant biomarkers in affordable microfluidic devices: Overcoming substrate diffusion limitations

Barbosa, Ana I.; Castanheira, Ana P.; Reis, Nuno M.

doi:10.1016/j.snb.2017.11.086

Cited by 19 publications

(9 citation statements)

References 39 publications

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“…RGB digital images of the immunoassay strips were split into three separated channel images by ImageJ software (NIH, Maryland). The blue channel images were used to calculate Abs values, based on the gray-scale peak height of each individual capillary of Teflon FEP MCF, as described previously. ,, Therefore, the absorbance signal is calculated for each capillary, according to the Beer–Lambert equation. The absorbance values presented averages of absorbance from 10 capillaries in a given MCF strip.…”

Section: Experimental Sectionmentioning

confidence: 99%

Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays

2021

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The performance of biosensors is often optimized in buffers, which brings inconsistencies during applications with biological samples. Current strategies for minimizing sample (matrix) interference are complex to automate and miniaturize, involving, e.g., sample dilution or recovery of serum/plasma. This study shows the first systematic analysis using hundreds of actual microfluidic immunoassay fluoropolymer strips to understand matrix interference in microflow systems. As many interfering factors are assay-specific, we have explored matrix interference for a range of enzymatic immunoassays, including a direct mIgG/anti-mIgG, a sandwich cancer biomarker PSA, and a sandwich inflammatory cytokine IL-1β. Serum matrix interference was significantly affected by capillary antibody surface coverage, suggesting for the first time that the main cause of the serum matrix effect is low-affinity serum components (e.g., autoantibodies) competing with high-affinity antigens for the immobilized antibody. Additional experiments carried out with different capillary diameters confirmed the importance of antibody surface coverage in managing matrix interference. Building on these findings, we propose a novel analytical approach where antibody surface coverage and sample incubation times are key for eliminating and/or minimizing serum matrix interference, consisting in bioassay optimization carried out in serum instead of buffer, without compromising the performance of the bioassay or adding extra cost or steps. This will help establishing a new route toward faster development of modern point-of-care tests and effective biosensor development.

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Section: Experimental Sectionmentioning

confidence: 99%

Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays

2021

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“…[11][12][13][14][15] Several kinds of polymers have been used to produce MCFs, including ethylene vinyl alcohol, [16] thermoplastic polyurethane, [5] polyethylene, [17] polylactic acid, [18] and fluorinated ethylene propylene. [19] MCFs can be produced with different numbers of microcapillaries, such as 7, 10, [20] 19, [21] and 28. [22] Recently, MCFs have been used mainly as microfluidic platforms with multiple microcapillaries in a single film strip.…”

Section: Introductionmentioning

confidence: 99%

Improved Size Uniformity of Microcapillaries in Microcapillary Films by Separate Gas Injection

Liu

Zhao

et al. 2021

Macro Materials & Eng

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Microcapillary films (MCFs) are microextrusion-processed plastic films containing an array of parallel microcapillaries, and have been widely utilized as microfluidic platforms. However, the nonuniform size distribution within a single MCF strip limits the application of MCFs in monodisperse droplet generation. In this paper, the mechanisms that lead to uneven microcapillary dimensions in conventional MCFs whose gas pressures in all channels are the same is first clarified. The effect of gas pressure in a single channel on the microcapillary dimensions in the film is then studied. Finally, the size uniformity of the microcapillaries is improved by separate gas injection in different channels, providing new possibilities for the generation of highly monodisperse microdroplets.

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“…In order to improve the limitations of current biosensors, they have been integrated with systems creating a plethora of lab‐on‐chip miniaturized diagnostic device platforms for point‐of‐care and multiplex biomarker quantification (Liao et al, 2018). One such device is microfluidics enables the processing of very small sample volumes (pL‐nL) in short times (min or s), improving the performance of diagnostic tests (Barbosa et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Current nanotechnology advances in diagnostic biosensors

et al. 2020

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Rapid, reliable and easy access diagnostics can significantly improve early detection and treatment monitoring of high mortality diseases, such as cardiovascular diseases, cancer, diabetes, among others (Rebelo et al., 2019). The successfully diagnostics of a disease, even prior to the manifestation of any symptom, can be crucial to efficacious treatment and survival rate (Chamorro-Garcia & Merkoçi, 2016).Biomarkers are frequently used to diagnose these diseases; however, they need to be quantified in a specific concentration range in physiological fluids, such as blood and urine, which is a time-consuming process that uses bulky and expensive laboratory equipment.Thus, there is a need for portable, rapid and reliable technologies that can simplify laboratory biomarker quantitation (Wu et al., 2017). In order to achieve this, a plethora of sensing mechanisms have been combined with biological elements, producing biosensor technologies, which enable the quantitation of analytes in mixtures.

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Sensitive optical detection of clinically relevant biomarkers in affordable microfluidic devices: Overcoming substrate diffusion limitations

Cited by 19 publications

References 39 publications

Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays

Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays

Improved Size Uniformity of Microcapillaries in Microcapillary Films by Separate Gas Injection

Current nanotechnology advances in diagnostic biosensors

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