Sensitive prostate specific antigen quantification using dihydrolipoic acid surface-functionalized phosphorescent quantum dots

García-Cortés, Marta; Fernández-Argüelles, Marı́a Teresa; Costa‐Fernández, José M.; Sanz‐Medel, Alfredo

doi:10.1016/j.aca.2017.08.003

Cited by 19 publications

(11 citation statements)

References 36 publications

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“…ZnS quantum dots (QDs) are IIB–VIA semiconductors and are considered to be good hosts when doped with Mn (3D transition metal ions) 1. Depending on their special luminescence property, Mn-doped ZnS QDs have been used for the detection of various substances by different surface modifications, including metal ions [copper(II), Fe(II), manganese(VII), and Pb(II)],2–5 environmental hazards (halobenzoquinones, patulin, florfenicol, and nicosulfuron),6–9 cocaine and its metabolites,10–12 drugs (atropine),13 biological molecules (thrombin,14 serotonin/5-hydroxytryptamine,15 dopamine,16 xanthine oxidase,17 prostate-specific antigen,18 glycoproteins19 and DNA20), and cancer cells 21. The widespread use of Mn-doped ZnS QDs has raised many toxicological concerns.…”

Section: Introductionmentioning

confidence: 99%

<p>Toxicity and serum metabolomics investigation of Mn-doped ZnS quantum dots in mice</p>

Yang

Wang

et al. 2019

IJN

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Purpose: Mn-doped ZnS quantum dots (QDs) with special luminescent properties have been widely researched and applied in various fields. Thus, their release toxicity and security cannot be ignored. Methods: In the present study, the toxicity and non-targeted metabolomics of Mn-doped ZnS QDs were investigated after single intravenous injection. Serum metabolites were evaluated based on gas chromatography-mass spectrometry together with multivariate statistical analyses [principal component analysis, partial least squares discriminant analysis, and orthogonal PLS-DA]. Results: The modified metabolites (variable importance in the projection (VIP) >1 and p<0.05) revealed that Mn-doped ZnS QDs exposure disturbed glycolysis, tricarboxylic acid cycle, ketoplasia, glutaminolysis, and amino acid and lipid metabolism. The behavior, coefficients of organs, and histological changes were the same as in the control group, and the disturbance of hematology and serum biochemistry was not dose-or time-dependent. Conclusion: Our study provides a general observation regarding the toxicity and potential metabolic responses of mice exposed to Mn-doped ZnS QDs.

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Section: Introductionmentioning

confidence: 99%

<p>Toxicity and serum metabolomics investigation of Mn-doped ZnS quantum dots in mice</p>

Yang

Wang

et al. 2019

IJN

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“…It is worth noting that a similar sandwich-type immunoassay for PSA determination has been previously evaluated using the native phosphorescent emission of Mn-ZnS QDs (before the catalytic gold deposition) as the analytical signal. The detection limit obtained using RTP detection was of 17 pg·mL −1 PSA [ 27 ]. In contrast, the catalytic gold deposition procedure here described resulted in an improvement in the detection limit for PSA quantification of 4 orders of magnitude (1.1 fg·mL −1 PSA).…”

Section: Resultsmentioning

confidence: 99%

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Cid-Barrio

Encinar

Costa‐Fernández

2020

Sensors

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A major challenge in the development of bioanalytical methods is to achieve a rapid and robust quantification of disease biomarkers present at very low concentration levels in complex biological samples. An immunoassay platform is presented herein for ultrasensitive and fast detection of the prostate-specific antigen (PSA), a well-recognized cancer biomarker. A sandwich type immunosensor has been developed employing a detection antibody labeled with inorganic nanoparticles acting as tags for further indirect quantification of the analyte. The required high sensitivity is then achieved through a controlled gold deposition on the nanoparticle surface, carried out after completing the recognition step of the immunoassay, thus effectively amplifying the size of the nanoparticles from nm to µm range. Due to such an amplification procedure, quantification of the biomolecule could be carried out directly on the immunoassay plates using confocal microscopy for measurement of the reflected light produced by gold-enlarged nanostructures. The high specificity of the immunoassay was demonstrated with the addition of a major abundant protein in serum (albumin) at much higher concentrations. An extremely low detection limit for PSA quantification (LOD of 1.1 fg·mL−1 PSA) has been achieved. Such excellent LOD is 2–3 orders of magnitude lower than the clinically relevant PSA levels present in biological samples (4–10 ng·mL−1) and even to monitor eventual recurrence after clinical treatment of a prostate tumor (0.1 ng·mL−1). In fact, the broad dynamic range obtained (4 orders of magnitude) would allow the PSA quantification of diverse samples at very different relevant levels.

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“…[113] To replace these ligands, a large number of hydrophilic ligands are used to improve water solubility of QDs. These hydrophilic molecules include mercaptocarbonic acids [HS-(CH2)n-COOH, n = 1-15], [114] 2-aminoethanethiol, [115] dithiothreitol, [116] dihydrolipoic acid, [117] oligomeric phosphines, [118] peptides, [119] and thiolated poly(ethylene glycol). [120] These substituting molecules normally have bifunctional groups by which they bind to the QD surface via one end and another anchoring end assists to improve water solubility and to provide attachment to other molecules such as proteins, drugs, or antibodies.…”

Section: Surface Ligand Exchangementioning

confidence: 99%

Quantum Dots: A Review from Concept to Clinic

Kargozar

Hoseini

Milan

et al. 2020

Biotechnology Journal

170

101

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Quantum dots (QDs) are semiconductor materials that have gained great interest due to their unique characteristics like optical properties. They are extensively being used in different areas, including solar cells, light-emitting diodes, laser technology, as well as biological and biomedical applications. In this review, comprehensive information about different aspects of QDs is provided, including their types and classifications, synthesis approaches, in vitro and in vivo toxicity, biological applications, and potentials in clinical applications. With a focus on the biological aspects, the respective in vitro and in vivo studies are collected and presented. Various surface modifications on QDs are discussed as directly influencing their properties like toxicity and optical abilities. Given the promising results, these materials are clinically used for targeted molecular therapy and imaging. However, there are a large number of questions that should be addressed before the wide application of QDs in a clinical setting. Regarding the existing barriers to QDs, suggestions are given and discussed to present an appropriate route for the clinical use of these materials.

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Sensitive prostate specific antigen quantification using dihydrolipoic acid surface-functionalized phosphorescent quantum dots

Cited by 19 publications

References 36 publications

<p>Toxicity and serum metabolomics investigation of Mn-doped ZnS quantum dots in mice</p>

<p>Toxicity and serum metabolomics investigation of Mn-doped ZnS quantum dots in mice</p>

Catalytic Gold Deposition for Ultrasensitive Optical Immunosensing of Prostate Specific Antigen

Quantum Dots: A Review from Concept to Clinic

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