Sensitive quantification of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry

Hofmann, Ute; Schwab, Matthias; Treiber, Gerd; Klotz, Ulrich

doi:10.1016/j.jchromb.2005.11.036

Cited by 42 publications

(27 citation statements)

References 11 publications

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“…Similarly, for the IS the m/z 370.1 precursor ion to the m/z 252.1 was used for quantification purposes. As the earlier publications (Kanazawa et al, 2002;Wang et al, 2005;Hofmann et al, 2006) have discussed extensively for the fragmentation pattern of OPZ, we are not presenting the data pertaining to this.…”

Section: Mass Spectroscopymentioning

confidence: 99%

“…The C max and AUC of OPZ are approximately proportional in doses up to 40 mg, but because of a saturable first-pass effect, a greater than linear response in C max and AUC occurs with doses greater than 40 mg. Absolute bioavailability (compared with intravenous administration) is about 30-40% at doses of 20-40 mg, due in large part to pre-systemic metabolism (Andersson, 1996). Several LC-MS/MS bioanalytical methods have been reported for estimation of OPZ alone or along with its metabolites in human biological samples (Woolf and Matuszewski, 1998;Stenhoff et al, 1999;Kanazawa et al, 2002;Yin et al, 2004;Frerichs et al, 2005;Wang et al, 2005;Hofmann et al, 2006;Song and Naidong, 2006;Hultman et al, 2007;Martens-Lobenhoffer et al, 2007). Although the reported LC-MS/MS methods are sensitive enough, the reported lowest limit of quantitation (LLOQ) amongst them was 0.4 ng/mL (Frerichs et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Highly sensitive method for the determination of omeprazole in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: application to a clinical pharmacokinetic study

Vittal

Ganneboina

Layek

et al. 2008

Biomedical Chromatography

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A highly sensitive, rapid assay method has been developed and validated for the estimation of omeprazole (OPZ) in human plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The assay procedure involves alkalinization of plasma followed by simple liquid-liquid extraction of OPZ and lansoprazole (internal standard, IS) from human plasma with acetonitrile. Chromatographic separation was achieved with 0.01 M ammonium acetate:acetonitrile (40:60, v/v) at a flow rate of 0.25 mL/min on an Inertsil ODS 3 column with a total run time 2.5 min. The MS/MS ion transitions monitored were 346.1 --> 198.1 for OPZ and 370.1 --> 252.1 for IS. Method validation and clinical sample analysis were performed as per FDA guidelines and the results met the acceptance criteria. The lower limit of quantitation achieved was 0.05 ng/mL and the linearity was observed from 0.05 to 10.0 ng/mL. The intra-day and inter-day precisions were in the ranges 2.09-8.56 and 5.29-8.19%, respectively. This novel method has been applied to a pharmacokinetic study of OPZ in humans.

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Section: Mass Spectroscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly sensitive method for the determination of omeprazole in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: application to a clinical pharmacokinetic study

Vittal

Ganneboina

Layek

et al. 2008

Biomedical Chromatography

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“…Lansoprazole and five metabolites were analyzed by HPLC with UV detection at 285 nm [14,17]. In addition, omeprazole and its major metabolites were determined using HPLC with UV detection at 302 nm [18][19][20][21][22] or HPLC with MS/MS [23,24]. Because the metabolites of either omeprazole or lansoprazole are inactive metabolites, their analysis is of limited importance in our pharmacokinetical context.…”

Section: Introductionmentioning

confidence: 99%

A bio‐analytical hydrophilic interaction LC‐MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children

Smet

Boussery

Cock

et al. 2010

J of Separation Science

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A hydrophilic interaction LC method with MS/MS was developed and validated for the simultaneous quantification of omeprazole and lansoprazole in human plasma. Chromatographic separation was achieved on a Betasil silica column using a high organic mobile phase (eluent A: ACN/formic acid 997.5:2.5 v/v; eluent B: water/formic acid 997.5:2.5 v/v) and gradient elution. The mass spectrometer was operated in the Multiple Reaction Monitoring mode. Prior to chromatography, liquid-liquid extraction with ethyl acetate was used and the organic layer was diluted with ACN, allowing direct injection on column. The method showed acceptable linearity, high precision (RSD%<10.5%), accuracy (88.9-109.3%) and selectivity in the two concentration ranges studied: 1.5-100 and 5-2000 ng/mL. The LOQ was established at 1.5 and 5 ng/mL for the two concentration ranges. Lack of variability in matrix effects was demonstrated and mean extraction recovery for omeprazole and lansoprazole was determined in the low (56.3-67.7%) and high (45.3-44.3%) concentration range, respectively. Additionally, plasma samples were found to be stable after three freeze-thaw cycles and for at least 15 h after extraction. This assay was successfully applied to a pharmacokinetic omeprazole study in children with cerebral palsy and mental retardation.

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“…Moreover, few publications have reported a simple HPLC method for the simultaneous determination of OME, HOME and OMES in human plasma [15][16][17][18][19][20][21][22][23]. HPLC methods includ- ing enantioselective separation have recently become a method of choice [24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Analysis of omeprazole and its main metabolites by liquid chromatography using hybrid micellar mobile phases

Rambla-Alegre

Esteve‐Romero

Carda‐Broch

2009

Analytica Chimica Acta

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Sensitive quantification of omeprazole and its metabolites in human plasma by liquid chromatography–mass spectrometry

Cited by 42 publications

References 11 publications

Highly sensitive method for the determination of omeprazole in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: application to a clinical pharmacokinetic study

Highly sensitive method for the determination of omeprazole in human plasma by liquid chromatography–electrospray ionization tandem mass spectrometry: application to a clinical pharmacokinetic study

A bio‐analytical hydrophilic interaction LC‐MS/MS method for the simultaneous quantification of omeprazole and lansoprazole in human plasma in support of a pharmacokinetic omeprazole study in children

Analysis of omeprazole and its main metabolites by liquid chromatography using hybrid micellar mobile phases

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