Sensitivitas Antimikroba Terhadap Bakteri Escherichia Coli Yang Diisolasi Dari Udang Di Pasar Keputran Surabaya

Abstract

The aimed of this study was to identify antibiotic Resistance of Escherichia coli isolated from Shrimp. There were 20 samples taken and isolated on surface of Eosin Methilen Blue Agar. E.coli produced metalic green colonies. Samples identified as E.coli based on macroscopic features and morphology of colonies, microscopic examination with gram staining and biochemical tests. The antibiotic sensitivity was determined through a standard antimicrobial disk diffusion test. The results of this study showed that 9 samples were bacteria of the spesies E. coli. Based on the results of antibiotic sensitivity test, it was found that 100 % of E. coli isolates were resistant to Amoxycillin 20 µg and 22,2 % of isolates are resistant to ampicillin 10 µg. Antibiotics Tetracycline 30 µg and Chloramphenicol 30 µg were 100 % sensitive. Gentamicin 10 µg showed that 77,8 % sensitive and 22,2 % intermediet. The precence of antibiotic resistance of betalaktam antibiotics makes it especially important to monitor antimicrobial susceptibility and mechanisms of resistance of E.coli isolated from shrimp, because new mechanisms of resistance occurring in animals may enter the food chain and its be transferred to the human. This importance of cooperation between sectors in order to monitor antimicrobial resistance.

UJI KEPEKAAN ANTIBIOTIK TERHADAP BAKTERI Salmonella sp. PADA JAGUNG BAKAR DI KAWASAN ULEE LHEU BANDA ACEH

“…Kemudian diambil cotton swab steril dicelupkan pada suspensi bakteri lalu dioleskan pada media MHA. Media didiamkan selama 30 menit, setelah itu cakram antibiotik kloramfenikol 30 µg dan amoxicillin 30 µg diambil menggunakan pinset steril lalu diletakkan di permukaan media, kemudian diinkubasi selama 24 jam pada suhu 37 o C. Diameter zona hambat yang terbentuk diukur menggunakan jangka sorong (Rahmaniar et al, 2019). Dilakukan pengulangan sebanyak 3 kali.…”

UJI RESISTENSI BAKTERI Escherichia coli DARI SUMBER AIR BAKU DI KARAWANG TERHADAP ANTIBIOTIK SIPROFLOKSASIN

“…Sebelum dilakukan pengamatan, hasil yang diperoleh dibandingkan dengan kontrol negatif berupa pelarut antibiotik yang digunakan yaitu akuades steril, sedangkan kontrol positif berupa antibiotik tetrasiklin yang masih sensitif terhadap E. coli. Pengujian pada tahap ini dilakukan sebanyak tiga kali (triplo) (CLSI 2020; Rahmaniar et al, 2019).…”

ISOLATION OF Escherichia coli FROM VANAME SHRIMP (Litopenaeus vannamei) POND AND THE SENSITIVITY TOWARD ANTIBIOTICS

“…Using chloramphenicol offers many advantages, including inhibiting disease development in vannamei shrimp farming, enhancing endurance, and increasing the weight of cultivated shrimp 5 . Research related to the bacterial sensitivity of E.coli against antibiotics was conducted by Rahmaniar et al 6 , which stated that E.coli bacteria are 100% resistant to the antibiotic amoxicillin and 22.2% to ampicillin. Meanwhile, the antibiotics that are still effective are tetracycline and chloramphenicol.…”