Sensitivities of the alternative respiratory components of potato tuber mitochondria to thiol reagents and Ca2+

Mariano, André Bellin; Valente, Caroline; Cadena, Sílvia Maria Suter Correia; Rocha, Maria Eliane Merlin; Oliveira, Maria Benigna Martinelli de; Carnieri, Eva Gunilla Skare

doi:10.1016/j.plaphy.2004.12.008

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…AOX is regulated by the reduction state of its substrate ubiquinol, being effectively outcompeted by OXPHOS complex III (ubiquinol:cytochrome c oxidoreductase) except when the reduced quinone reaches a substantial proportion of the total quinone pool (Dry et al ; Hoefnagel and Wiskich, ); this has been verified for the Ciona enzyme when expressed in mouse, under conditions of complex IV (cytochrome c oxidase) deficiency (Dogan et al ). In plants, AOX is also regulated by pyruvate (Hoefnagel and Wiskich ; Sierra‐Campos et al ; Carré et al ; Chen et al ) which affects its quaternary structure, and in diverse taxa by other organic acids (Siedow and Umbach ; Selinski et al ), nucleotides (Sierra‐Campos et al ; Selinski et al ), and calcium (Mariano et al ; Zalutskaya et al ). Despite the metabolic disturbances resulting from fat‐rich diets (Barrington et al ; Fujisaka et al ; Hassan et al ; Lee et al ; Showalter et al ; Tomášová et al ; Gu et al ), including the production of ketone bodies and many compounds included within or related to the aforementioned categories, it was not possible to determine whether or not AOX was enzymatically active in vivo , during the present study.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic effects of dietary stress in combination with a respiratory chain bypass in mice

et al. 2019

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The alternative oxidase (AOX) from Ciona intestinalis was previously shown to be expressible in mice and to cause no physiological disturbance under unstressed conditions. Because AOX is known to become activated under some metabolic stress conditions, resulting in altered energy balance, we studied its effects in mice subjected to dietary stress. Wild‐type mice ( Mus musculus , strain C57BL/6JOlaHsd) fed a high‐fat or ketogenic (high‐fat, low‐carbohydrate) diet show weight gain with increased fat mass, as well as loss of performance, compared with chow‐fed animals. Unexpectedly, AOX‐expressing mice fed on these metabolically stressful, fat‐rich diets showed almost indistinguishable patterns of weight gain and altered body composition as control animals. Cardiac performance was impaired to a similar extent by ketogenic diet in AOX mice as in nontransgenic littermates. AOX and control animals fed on ketogenic diet both showed wide variance in weight gain. Analysis of the gut microbiome in stool revealed a strong correlation with diet, rather than with genotype. The microbiome of the most and least obese outliers reared on the ketogenic diet showed no consistent trends compared with animals of normal body weight. We conclude that AOX expression in mice does not modify physiological responses to extreme diets.

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Section: Discussionmentioning

confidence: 99%

Phenotypic effects of dietary stress in combination with a respiratory chain bypass in mice

et al. 2019

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“…Mitochondria isolated from potato tubers have been used in several calcium-related studies (13,14,34).…”

Section: Physiological Fragmentation Of Mitochondria On Ca 2؉ Overloadmentioning

confidence: 99%

Magic‐angle phosphorus NMR of functional mitochondria:in situmonitoring of lipid response under apoptotic‐like stress

et al. 2009

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Using a noninvasive, solid-state magic-angle spinning nuclear magnetic resonance (MAS NMR) approach, we track ex vivo the behavior of individual membrane components in isolated, active mitochondria (model system: potato tubers) during physiological processes. The individual phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cardiolipin (CL) membrane constituents were identified as distinct lines by applying MAS (31)P NMR on extracted lipid membranes. However, the CL NMR signal appeared to be very broad in functional mitochondria, indicating a tight complex formation with membrane protein. Calcium stress induced severe membrane degradation without recovery of a single CL NMR resonance. This suggests that calcium overload destroys the outer mitochondrial membrane and does not modify strongly the CL protein complexes in the inner membrane; a conclusion confirmed by respiratory controls. Conversely, mitochondrial membrane disruption on time degradation or mechanical stress generates clearly visible identical CL NMR signals, similar to those observed in rehydrated lipid extracts. Similarly, noninvasive based NMR tracking of lipids in response to diverse physiological stimuli can easily be used for other organelles and whole living cells.

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“…As mitocôndrias de células de Rubus fruticosus foram isoladas através de centrifugações diferenciais baseadas em uma técnica de isolamento de mitocôndrias de culturas embriogênicas de Araucaria angustifólia (Mariano, 2005) (AKERMAN & WIKSTRON, 1976).…”

Section: Isolamento De Mitocôndrias De Células De Rubus Fruticosusunclassified

Efeito do extrato de Azadirachta indica (nim) sobre resposta de hipersensibilidade mediada por ácido salicílico em células de Rubus fruticosus

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Sensitivities of the alternative respiratory components of potato tuber mitochondria to thiol reagents and Ca2+

Cited by 8 publications

References 27 publications

Phenotypic effects of dietary stress in combination with a respiratory chain bypass in mice

Phenotypic effects of dietary stress in combination with a respiratory chain bypass in mice

Magic‐angle phosphorus NMR of functional mitochondria:in situmonitoring of lipid response under apoptotic‐like stress

Efeito do extrato de Azadirachta indica (nim) sobre resposta de hipersensibilidade mediada por ácido salicílico em células de Rubus fruticosus

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