Sensitivity Analysis of Italian Lolium spp. to Glyphosate in Agricultural Environments

Panozzo, Silvia; Collavo, Alberto; Sattin, M.

doi:10.3390/plants9020165

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…The similarity of their responses could be due to the low concentrations of glyphosate that were used here. Previous studies that have reported intraspecific variation in plant tolerance to glyphosate have usually examined direct responses to herbicide application based on dose-response bioassays (e.g., Barroso et al 2010;Panozzo et al 2020), in which plants are typically exposed to much higher concentrations of glyphosate than those used here. Overall, our results support the previous finding that plants may be less sensitive to glyphosate residues than their associated micro-organisms (Claassens et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Glyphosate residues alter the microbiota of a perennial weed with a minimal indirect impact on plant performance

et al. 2021

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Purpose In cold climates, glyphosate residues may linger in soils, with effects on plant–microbe interactions and, consequently, plant performance. Here, we explore the influence of glyphosate residues on the endophytic microbiota (bacteria and fungi) and performance of the perennial nitrogen-fixing weed Lupinus polyphyllus. Methods In a common garden, we grew plants from six populations of L. polyphyllus in glyphosate-treated or untreated control soils, with or without additional phosphorus. We sampled plant microbiota (leaves, roots, nodules) and assessed plant performance based on six traits: height, retrogression probability (i.e. shrinkage), biomass, root:shoot ratio, nodule number, and nodule viability. Results The richness of plant endophytic microbial communities was determined by soil phosphorus level rather than by glyphosate treatment. However, for bacteria, the composition of these communities differed between glyphosate-treated and control soils across plant tissue types; no difference was observed for fungi. The plant bacterial communities in both soil types were dominated by potential nitrogen-fixing bacteria belonging to family Bradyrhizobiaceae, and particularly so in glyphosate-treated soils. Overall, though, these changes in plant bacterial communities had a minor effect on plant performance: the only difference we detected was that the probability of retrogression was occasionally higher in glyphosate-treated soils than in control soils. Conclusion Our findings indicate that glyphosate-based herbicides, when applied at the recommended frequency and concentration, may not have critical effects on the growth of short-lived weeds after the safety period has passed; however, the endophytic microbiota of such weeds may experience longer-lasting shifts in community structure.

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Section: Discussionmentioning

confidence: 99%

Glyphosate residues alter the microbiota of a perennial weed with a minimal indirect impact on plant performance

et al. 2021

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“…Therefore, the application time is crucial for reaching satisfactory control with the lowest herbicide doses. On the other hand, it is well-known that besides the weed species and the growing period of the weeds, ecological conditions and weed populations [48] significantly affect the impact of herbicides. There can be important variation among the weed populations.…”

Section: Discussionmentioning

confidence: 99%

Determination of Minimum Doses of Imazamox for Controlling Xanthium strumarium L. and Chenopodium album L. in Bean (Phaseolus vulgaris L.)

Gürbüz

Yentürk

2022

Agronomy

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This study was conducted to investigate the minimum doses of the imazamox active ingredient (ai) that provide satisfactory efficacy (>90%) against fat hen (Chenopodium album L.) and common cocklebur (Xanthium strumarium L.). These two weeds are among the most troublesome weeds of bean fields. The minimum dose studies were carried out separately in the 2–4 and 6–8 true leaf stages of both weeds. The experiments were carried out in pots under greenhouse conditions. The experiments were repeated three times. In the first two experiments, the recommended dose of imazamox (100%) together with 75%, 50% and 25% doses were applied to the weeds during the above-mentioned leaf stages. Some pots were left untreated for control. In the third experiments, 12.50% and 6.25% of the recommended doses were also tested. Plant height and the number of leaves were recorded on the 1st, 3rd, 5th, 7th, 14th, 21st and 28th days following the herbicide application. As a result of the studies, it was determined that nearly half the recommended dose (48.18 g ai/da) provides 90% success in the control of common cocklebur (X. strumarium) when applied at the 2–4 true leaf stages, while a lower dose (36.11 g ai/da) is required for obtaining the same control when applied at the 6–8 true leaf stages. For the fat hen (C. album), only a 17.69 g ai/da application dose was found to provide 90% control at the period of 2–4 true leaves, while 21.21 g ai/da was noted to provide 90% control when applied at the 6–8 true leaf stage. The results suggest that the increase in leaf area reduces the imazamox requirement for the control of X. strumarium.

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“…hold many characteristics, (i.e., high genetic diversity and ability to exchange genetic material due to gene flow) that enable them to evolve resistance mechanisms to a plethora of different herbicides. On the other hand, glyphosate is one of the most efficient post-emergence herbicides, mainly in cropping systems targeting grasses [38]. For all these reasons, advancing our knowledge on TSR and NTSR mechanisms of Lolium spp.…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Rapid Molecular Analyses to Identify Glyphosate Resistance in Lolium spp.

et al. 2021

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Lolium spp. are troublesome weeds mainly found in winter cereal crops worldwide, including Europe. In recent years resistant mechanisms have been evolved to several important herbicides. In this study we investigated the mechanisms responsible for conferring glyphosate resistance in some Lolium spp. populations. A holistic approach was used, based on dose-response experiments, determination of shikimic acid concentration in plant leaf tissue, as well as molecular analyses. More specifically, in three Lolium spp. populations the existence of a mutation in the Pro-106 codon of the 5-enolpyruvylshikimate-3 phosphate synthase (EPSPS) gene was investigated as well as the relative transcript levels of four ABC-transporter genes were monitored at three time points after glyphosate application. The results demonstrated that glyphosate resistance is a multifactor phenomenon. Relative transcript levels of the ABC-transporter genes were abundant at very early time points after glyphosate treatments. Dose-response experiments and shikimate analyses were in accordance with the findings of the quantitative PCR (qPCR) analyses. We suggest that relative expression ratio of ABC-transporter genes can be a useful tool to rapidly identify Lolium spp. populations resistant to glyphosate.

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Sensitivity Analysis of Italian Lolium spp. to Glyphosate in Agricultural Environments

Cited by 8 publications

References 33 publications

Glyphosate residues alter the microbiota of a perennial weed with a minimal indirect impact on plant performance

Glyphosate residues alter the microbiota of a perennial weed with a minimal indirect impact on plant performance

Determination of Minimum Doses of Imazamox for Controlling Xanthium strumarium L. and Chenopodium album L. in Bean (Phaseolus vulgaris L.)

Biochemical and Rapid Molecular Analyses to Identify Glyphosate Resistance in Lolium spp.

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