This work designs a novel dielectric modulated step channel Junctionless tunnel eld effect (DM-SC-JLTFET) for the label-free detection of breast cancer cells using their dielectric constant (K) values. The dielectric modulation technique is exploited to detect breast cancer cells (BCC) whose K values are observed at 200 MHz frequency using an open-ended coaxial probe technique. The charge plasma concept is employed to suppress the random dopant uctuation (RDF). The usage of this concept rendering the complex fabrication process simple and affordable. A novel step channel structure has been implemented with reduced substrate thickness for the TFET device that improves the e cacy of the biosensor. The proposed device uses on-current (I on ) and ambipolar current (I amb ) to measure the sensitivity of cancer biomolecules. An in-depth analysis has been carried out for the biosensor by considering performance parameters such as the electrostatics of the device, energy band diagram, lateral electric eld, and threshold voltage (V th ). The device sensitivity is analyzed using parameters like I on /I off , I off /I amb current ratio, Subthreshold Swing (SS), and V th . The proposed device reports high detection sensitivity of 2.683x10 6 and a low SS of 32 mV/dec for breast cancer cell biomolecule T47D (K = 32), effectively reducing the RDF effect. The simulated device shows enhanced sensitivity and higher compatibility for breast cancer cell detection, and this device will be an excellent alternative to classical vivo breast cancer detection.