Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: A comparison using control sera obtained from healthy individuals and patients with multiple medical problems

Wichainun, Ramjai; Kasitanon, Nuntana; Wangkaew, Suparaporn; Hongsongkiat, Sith; Sukitawut, Waraporn; Louthrenoo, Worawit

doi:10.12932/ap0272.31.4.2013

Cited by 71 publications

(45 citation statements)

References 30 publications

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“…It was clearly demonstrated that sensitivity and specificity of the test were 74 and 100%, respectively. Many of previous studies recorded high specificity of ELISA anti-dsDNA antibodies in SLE patients (Wichainun et al, 2013). Three sophisticated techniques were previously evaluated by multiple studies for the detection of anti-ds DNA antibodies in SLE patients; radioimmunoassay, immunofluorescent and ELISA techniques.…”

Section: Resultsmentioning

confidence: 99%

“…Deposition of anti-dsDNA antibodies in certain renal structures has been linked to disease severity (Yung and Chan, 2008). High sensitivity and specificity of anti-dsDNA antibodies were concluded by various studies concerning with accurate and early detection of renal involvement in lupus patients (Wichainun et al, 2013). However, a negative anti-dsDNA findings may be observed in many patients suspected with LN.…”

Section: Introductionmentioning

confidence: 99%

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Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Manhal¹,

Mohammed²

2016

American Journal of Immunology

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Abstract:The majority of patients with Lupus Nephritis (LN) usually have abnormal findings of kidney function tests. Severe glomerular damage may be observed in some patients and requires prompt therapeutic interventions. High titer levels of anti-ds-DNA antibodies may correlate to some extent with disease activity in lupus nephritis patients. The purpose of this study was to evaluate the relationship between anti-ds-DNA antibody titers and abnormal kidney function tests in patients with lupus nephritis. A total of seventy patients with lupus nephritis and fifty healthy controls were enrolled in this study. Blood samples were collected and labeled from study patients and controls for certain hematological, biochemical and immunological investigations. Anti-ds-DNA antibodies were tested by using IgG-ELISA test. It was shown that 74% of lupus nephritis patients showed positive results for anti-ds-DNA antibodies in their serum specimens (p-value <0.01). Sensitivity and specificity of the anti-ds-DNA antibody test for the diagnosis of lupus nephritis were 74 and 100%, respectively. Anaemia, hypoalbuminemia, fasting hyperglycemia and elevated blood urea nitrogen were significantly associated with lupus nephritis activity. Further studies are required to study genomic and unprecedented biomarkers associated with anti-ds-DNA antibodies in patients with lupus nephritis to develop our perception of this autoimmune disease.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Manhal¹,

Mohammed²

2016

American Journal of Immunology

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“…3 Other studies calculated the specificity and sensitivity of ANA testing in diagnosing SLE as 92% and 98%, respectively. 4 Substituting these values into the calculations in the eTable in the Supplement gave a positive predictive value of 5.47% and negative predictive value of 99.99% (calculations not shown). Even with this improved sensitivity and specificity, for every true-positive ANA test result, there were 19 false-positive test results.…”

Section: Discussionmentioning

confidence: 99%

Association of Systemic Lupus Erythematosus With Uveitis

2015

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ystemic lupus erythematosus is a systemic autoimmune condition that can be associated with uveitis. The prevalence of SLE as a cause of uveitis varies in the literature. In 1990, Rosenbaum and Wernick 1 reported on the use of routine antinuclear antibody (ANA) testing for patients with uveitis. Their Bayesian analysis required a pretest probability of SLE in patients with uveitis. Rosenbaum and Wernick 1 estimated a prevalence of SLE as a cause of uveitis as 0.1%. This estimate was based on 1218 patients in 3 studies in the 1980s where SLE was neither found nor explicitly mentioned. Based on this prevalence, they estimated that with a positive ANA test result in a patient with uveitis, the chance of the patient having SLE was less than 1%. They recommended against the routine screening of patients with uveitis with ANA testing in the absence of other features suggestive of SLE. A higher pretest probability resulted in a higher posttest probability.Rodriguez et al 2 described the etiology of uveitis in 1237 patients attending the Massachusetts Eye and Ear Infirmary, reporting the prevalence of SLE as 4.8%. Substituting this value for pretest probability into the Bayesian analysis performed by Rosenbaum and Wernick 1 resulted in a 24% posttest probability of having SLE. Thus, the prevalence of SLE in uveitis determines the value of routine ANA testing.

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“…Anti-double stain antibodies (anti-dsDNA) is rarely used for SLE screening, because the sensitivity is only 70%, however anti-dsDNA has high specificity -99 % .On the first admission the patient had negative anti-dsDNA test. [11][12][13] The patient had low C3 level, which is one of the diagnostic criteria of SLE. During pregnancy compliment C3 and C4 level, increased due to oestrogen induced synthesis in the liver.…”

Section: Discussionmentioning

confidence: 99%

New onset of systemic lupus erythematosus during pregnancy

Keisa¹,

Boka

Vitina

et al. 2016

Int J Reprod Contracept Obstet Gynecol

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Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: A comparison using control sera obtained from healthy individuals and patients with multiple medical problems

Abstract: ANA and anti-dsDNA gave high sensitivity and high specificity in patients with SLE, even when using MMP patient's sera as controls. Physicians should take care in interpreting ANA and anti-dsDNA results in MMP patients who do not have signs or symptoms of SLE or connective tissue diseases.

Cited by 71 publications

References 30 publications

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Relationship between Anti-Ds-DNA Antibodies and Abnormal Kidney Function Tests in Patients with Lupus Nephritis

Association of Systemic Lupus Erythematosus With Uveitis

New onset of systemic lupus erythematosus during pregnancy

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